Supplementary Data for: Liu Z, Bethunaickan R, Huang W, Lodhi U, Solano I,
Madaio MP, and A Davidson. Interferon alpha Accelerates Murine SLE in a T
Cell Dependent Manner. Arthritis and Rheumatism. 2010 Oct 15. [Epub ahead of
print]PMID: 20954185 [PubMed - as supplied by publisher].
Supplementary Table 1: Quantitative real time PCR of spleen cytokines
Naïve (n=14)
IFNa (n=14) Anti-CD4 (n=7)
1.00 +/- 0.96a
2.21 +/- 2.31
0.34 +/- 0.44b
IL-4
1.00 +/- 0.67c
5.93 +/- 7.30
2.43 +/- 2.35
IL-6
b
1.00 +/- 0.78
4.76 +/- 1.71
0.67 +/- 0.72b
IL-10
1.00 +/- 0.59
1.24 +/- 1.14
1.02 +/- 1.08
IL-12
1.00 +/- 0.88
8.58 +/- 14.78
1.09 +/- 1.19
IL-17
1.00 +/- 1.01c
3.86 +/- 1.79
0.29 +/- 0.43c
IL-21
1.00 +/- 0.73c
2.85 +/- 1.43
0.72 +/- 0.22c
IFNγ
1.00 +/- 0.54
1.19 +/- 0.42
0.62 +/- 0.34b
TNFα
p values are compared with IFNα 19wk mice. Only significant p values are shown.
p < 0.05, b p < 0.01, c p < 0.001. Data are pooled from two experiments.
a
Supplementary Table 2. Serum levels of selected cytokines
Serum
Naïve 12wk
Naïve 19wk (n=4) IFNα 19wk (n=5)
IFNα 19wk +
levels
(n=5)
anti-CD4 (n=5)
(pg/mL)
BAFF
8003.9 ± 719.9 b 7646.9 ± 1020.9 b 11788.8 ± 1105.3 13904.7 ± 1947.8
IL-6
4.4 ± 2.7 b
4.2 ± 2.1 a
21.5 ± 9.5
74.1 ± 105.5
IL-10
5.6 ± 1.0 b
5.3 ± 0.8 a
20.2 ± 12.1
80.3 ± 160.8 c
TNF-α
4.9 ± 1.8 b
3.2 ± 1.3 a
25.9 ± 14.1
8.6 ± 2.3 b
Sera were immediately frozen and stored at -80oC. Quantitative assays of cytokines in the serum
were performed by AssayGate (Ijamsville, MD). p values of are compared with IFNα 19wk
mice. Only significant p values are shown. a p < 0.02, b p < 0.01 c one outlier in each of the 2
experiments. Data are representative of two experiments.