TSA Signal Amplification
(TSA) Systems
See the difference with TSA —
the ultimate technology for unparalleled
detection sensitivity
For unparalled sensitivity,
TSA technology
Increase your immunohistochemistry and in situ hybridization detection capabilities
with PerkinElmer’s proprietary Tyramide Signal Amplification technology (TSA™) —
and see clearly why TSA is the standard for superior IHC and ISH sensitivity in
labs around the world.
The remarkable sensitivity enhancements that TSA delivers let you see previously
undetectable levels of protein or nucleic acid — for invaluable new information and
insight into your research. Hundreds of technical publications to date provide proof
of the sensitivity, versatility and applicability of TSA technology to a wide range of
scientific research fields. Add TSA to your protocols and you, too, will
see the difference.
Tyramide Signal Amplification is a patented* technology invented by PerkinElmer scientists that amplifies both
chromogenic and fluorescent signals in many applications. TSA can be used in any application that allows the
addition of horseradish peroxidase (HRP) into its protocol, such as immunohistochemistry, in situ hybridization,
ELISA and microarray-based differential gene and protein expression studies. In standard IHC and ISH protocols,
use of TSA results in a significant increase in sensitivity, without loss of resolution or increase in background.
*TSA is protected by US patents: 5,731,158, 5,583,001, and 5,196,306 and foreign equivalents.
PerkinElmer is the world’s sole source of patented TSA tech
2
makes all the difference
Quick Glance
Innovative amplification technology maintains signal clarity
TSA is easily integrated into any protocol after an
initial addition of horseradish peroxidase (HRP).
The HRP is used to catalyze the deposition and
binding of a labeled (e.g., biotin, DNP or other
labeling moieties) tyramide onto tissue sections
or cell preparations previously blocked with
proteins. This reaction is quick (less than 10
minutes). The binding is covalent because the
reaction intermediately dimerizes with tyrosine
residues on the surface-bound endogenous
proteins. These labels can then be detected
by standard chromogenic or fluorescent techniques. Since the added labels are only deposited proximal to the enzyme site, there is minimal,
if any, loss of resolution.
Diagram of TSA direct fluorescence detection.
hnology — the standard for superior IHC and ISH sensitivity.
w w w. p e r k i n e l m e r. c o m
3
See the difference in your
TSA can provide numerous benefits when you add it to your immunohistochemistry or
Increase Sensitivity
Conserve Antibody or Probe
Maximizing the sensitivity of methods aimed at the
cellular localization of proteins and nucleic acids is of
particular importance when target levels are known or
suspected to be low. Addition of TSA to your protocol
may reveal localized targets previously undetectable
and therefore unsuspected — without loss of resolution
or increase in background.
Application of TSA may be beneficial even when an
increase in sensitivity is not required because TSA
allows use of less antibody or probe. Typically, the
same level of sensitivity can be achieved with a
significant reduction in the amount of target antibody
(up to 1,000-fold) or probe used.
CHR 15 D2
Gene mapping of c-myc,
exon 2 gene (855 bp)
mapped to chromosome
15, band D2, mouse
metaphase chromosomes.
Sensitivity improvement
with TSA allows use of
smaller (cDNA, oligos)
probes for more precise
localization. Detection
of single copy genomic
sequence was achieved
using a probe less
than 2 kb.
A
B
IHC of EBV antigen in Hodgkin’s Lymphoma of mixed cellularity.
Comparison shows (A) Anti-EBV dilution 1:25, without TSA
enhancement, (B) Anti-EBV dilution 1:25,000, enhanced by TSA.
(Courtesy of R. Von Wasielewski and S. Gignac, Pathologisches
Institut de Medizinischen Hochscule, Hannover, Germany.)
Do Multi-Target Detection
Eliminate PCR
TSA systems have been designed to allow utilization
of various chromogens or fluorophores. This flexibility
facilitates applications in which multi-target detection
in the same sample is of interest.
TSA is simpler and faster than IS-PCR with equivalent
sensitivity. It detects even low or single copy genes.
No special equipment is required.
Multi-target detection
enables simultaneous
visualization of
multiple targets.
A
B
Detection of HIV-1 DNA in formaldehyde-fixed lymph node
tissues from positive autopsy material. (A) In situ PCR, 20 cycles.
(B) TSA-enhanced ISH. (Courtesy of Dr. J. Luka and C. Afflerbach,
Eastern Virginia Medical School, Norfolk, VA.)
4
research with TSA in your protocol
in situ hybridization protocols. Use of TSA is particularly valuable when you need to:
Obtain High Resolution
Get Faster Results
TSA provides exquisite resolution — for clearer images
than other methods.
See results within a day versus up to a week for
radiometric detection, and achieve equivalent
sensitivity and resolution.
A
B
Comparison of direct immunofluorescent staining and TSAenhanced direct fluorescent staining of CMV infected cells.
(A) Direct fluorescent staining. (B) Direct fluorescent staining
enhanced by TSA.
A
Reduce Interference
Biotin-free TSA Plus DNP Systems provide a simple
protocol and eliminate biotin quenching procedures.
Biotin-free formats also eliminate background problems.
B
Comparison of radiometric and TSA-amplified ISH detection
of muscarinic receptor mRNA in rat brain hippocampus. M-1
oligo-nucleotide probes were labeled with 33P and fluoresceinN6-ddATP by 3' end-labeling and were hybridized to frozen rat
brain tissue sections. (A) Autoradiography of 33P was carried
out for one month. (B) TSA amplification with DAB.
A
B
Detection of p53 mRNA in lung tissue. Digoxigenin-labeled p53
RNA probes were hybridized to paraffin-embedded lung tissue.
Comparison shows (A) standard digoxigenin detection with
alkaline phophatase/BCIP-NBT (60 minute substrate incubation),
and (B) TSA Plus DNP with alkaline phosphatase/BCIP-NBT
(15 minute substrate incubation).
w w w. p e r k i n e l m e r. c o m
5
TSA Direct Fluorescence Detecti
Immunohistochemistry
P
HR
Detection of Target
Key:
SA
B
F
T
SA
HRP
AP
B
2˚
Ab
1˚ Ab
=
=
=
=
=
=
Biotin
Fluorophore
Tyramide
Streptavidin
Horseradish Peroxidase
Alkaline Phosphatase
Amplification
and Visualization
Procedures
Actual Time
Added to
Protocol
Blocking Step
30 min
Wash Step
3 x 5 min
Incubation of
fluorophore tyramide
3 – 10 min
Wash Step
3 x 5 min
BLOCKING REAGENT
TARGET
F
Signal Amplification
T
F
T
SA
F
HR
T
F
P
T
T
Production
of Reactive
Tyramide
by HRP
F
B
2˚
Ab
F
F
T
T
1˚ Ab
F
F
T
T
Visualization via Fluorescence Microscopy
Mouse Brain, 20x magnification, 2 second exposure
Conventional detection with
Cyanine 3 conjugated 2˚ antibody.
Dilution 1:1,000
6
Standard TSA Cyanine 3.
Dilution 1:100,000
TSA Plus Cyanine 3.
Dilution 1:10,000,000
on
In Situ Hybridization
Key:
Detection of Target
H
R
B
F
T
SA
HRP
AP
D
P
SA
=
=
=
=
=
=
=
Biotin
Fluorophore
Tyramide
Streptavidin
Horseradish Peroxidase
Alkaline Phosphatase
Digoxigenin
Amplification
and Visualization
Procedures
Actual Time
Added to
Protocol
Blocking Step
30 min
Wash Step
3 x 5 min
Incubation of
fluorophore tyramide
3 – 10 min
Wash Step
3 x 5 min
PROBE
B
BLOCKING REAGENT
TARGET
Signal Amplification
F
F
T
F
T
H
R
P
Production
of Reactive
Tyramide
by HRP
T
SA
F
F
T
T
B
F
F
T
T
Visualization via Fluorescence Microscopy
Embryonic mouse
brain histone H4
mRNA ISH, using TSA
Plus Cyanine 3 and
MAP2 immunoreactivity
(IHC) with TSA Plus
Fluorescein System.
w w w. p e r k i n e l m e r. c o m
7
TSA Indirect Chromogenic or Fluo
(Biotin-based or Biotin-free)
Immunohistochemistry
HR
Detection of Target
P
SA
B
2˚
Amplification
and Visualization
Procedures
Actual Time
Added to
Protocol
Blocking Step
30 min
Wash Step
3 x 5 min
Incubation of
biotinyl tyramide
3 – 10 min
Wash Step
3 x 5 min
Addition of
enzyme conjugate
30 min
Wash Step
3 x 5 min
Ab
1˚ Ab
BLOCKING REAGENT
B
TARGET
T
B
HR
T
B
B
T
T
Ab
1˚ Ab
Detection of
Amplified
Signal
B
B
B
B
B
T
T
HR
HR
P
SA
T
T
SA
T
B
P
HR
Ab
SA
1˚ Ab
B
B
T
T
Visualization
SA
SA
B
T
T
SA
B
P
HR
Ab
SA
1˚ Ab
HR
P
T
P
B
HR
HR
P
T
2˚
SA
T
SA
B
SA
8
HR
HR
P
SA
B
T
B
HR
P
B
Incubation of
chromogen or
fluorophore
P
HR
HR
P
SA
P
SA
B
B
HR
P
HR
P
P
HR
HR
P
T
HR
SA
B
SA
B
T
T
2˚
Production
of Reactive
Tyramide
by HRP
B
B
SA
P
SA
HR
P
SA
T
SA
T
2˚
B
P
T
Signal
Amplification
B
B
T
T
P
rescence Detection
(Biotin-based or Biotin-free)
In Situ Hybridization
Detection of Target
H
R
P
SA
Amplification
and Visualization
Procedures
Actual Time
Added to
Protocol
Blocking Step
30 min
Wash Step
3 x 5 min
Incubation of
biotinyl tyramide
3 – 10 min
Wash Step
3 x 5 min
Addition of
enzyme conjugate
30 min
Wash Step
3 x 5 min
PROBE
B
BLOCKING REAGENT
TARGET
Signal
Amplification
B
Production
of Reactive
Tyramide
by HRP
B
T
B
H
T
R
P
T
SA
B
B
T
T
B
B
B
T
T
Detection of Amplified Signal
HR
HR
P
P
SA
B
T
B
B
T
T
SA
SA
B
P
HR
Incubation of
chromogen or
fluorophore
SA
SA
SA
P
P
T
HR
P
B
HR
HR
HRP
SA
B
B
T
T
Visualization
HR
HR
P
P
SA
T
B
T
B
B
T
T
SA
B
SA
P
HR
SA
SA
SA
HR
P
B
HR
P
P
HRP
HR
SA
B
B
T
T
w w w. p e r k i n e l m e r. c o m
9
Visualization Options for Indirect
Immunohistochemistry
Chromogenic with SA-HRP
SA
P
HR
T
B
P
T
SA
P
HR
SA
T
P
HR
SA
B
T
T
1˚ Ab
P
HR
B
B
T
T
Chromogenic with SA-AP
AP
SA
B
B
T
T
T
B
AP
B
B
T
T
Fluorescence with
SA-Fluorophore
F
SA
B
T
B
F
T
SA
T
B
T
T
B
B
F
2˚
Ab
F
SA
1˚ Ab
P
HR
T
SA
10
B
SA
B
F
SA
SA
Addition of SA-Fluorophore
(SA-Fluorescein)
(SA-Coumarin)
(SA-Texas Red®)
viewed by fluorescence microscopy
SA
F
F
SA
B
AP
SA
1˚ Ab
AP
SA
B
P
HR
SA
B
AP
AP
T
2˚
Ab
SA
SA
T
B
T
Addition of SA-AP followed by
alkaline phosphatase catalyzed chromogen
SA
AP
AP
SA
Addition of SA-HRP followed by
horseradish peroxidase catalyzed
chromogen
SA
B
SA
After signal amplification, the deposited
biotin can be directly visualized by any
of the following methods:
B
P
2˚
Ab
SA
SA
B
HR
HR
P
HR
P
B
T
Amplification
and Visualization
Procedures
B
SA
HR
P
HR
B
B
T
T
F
SA
NOTE: TSA and Avidin Biotin Complex (ABC) —
TSA can be integrated into systems currently using
the ABC method, either before or after the ABC step.
Detection
In Situ Hybridization
Chromogenic with SA-HRP
HR
HR
P
Amplification
and Visualization
Procedures
P
SA
T
B
B
T
T
HR
SA
SA
B
SA
SA
SA
P
P
T
B
HR
P
B
HR
HR
HRP
SA
B
B
T
T
P
After signal amplification, the deposited
biotin can be directly visualized by any
of the following methods:
Addition of SA-HRP followed by horseradish
peroxidase catalyzed chromogen
Chromogenic with SA-AP
AP
AP
SA
SA
T
B
B
T
T
SA
SA
B
AP
SA
SA
AP
P
T
B
SA
Addition of SA-AP followed by alkaline
phosphatase catalyzed chromogen
B
HR
AP
AP
B
B
T
T
Addition of SA-Fluorophore
(SA-Fluorescein)
(SA-Coumarin)
(SA-Texas Red®)
viewed by fluorescence microscopy
Fluorescence with SA-Fluorophore
F
F
SA
SA
T
B
B
T
T
SA
B
F
SA
SA
SA
SA
F
HR
T
B
P
B
F
F
B
B
T
T
Probe Labeling Option
P
HR
SA
B
H
R
P
H
Ab
Ab
F
D
R
P
w w w. p e r k i n e l m e r. c o m
11
There’s a
TSA system
PerkinElmer offers a variety of TSA systems to enable every lab to benefit from
the sensitivity enhancements of this outstanding technology. You’ll maximize the
difference that TSA makes in your IHC and ISH applications when you select a TSA
system based on your research requirements and the experience of your lab.
First, consider your research requirements:
• Select chromogenic or fluorescence detection based on your laboratory equipment and your need
for a permanent record.
• Eliminate biotin interference by considering the level of endogenous biotin in your specimens
and your desire to reduce background or eliminate biotin-quenching procedures.
• Choose from several fluorophores based on your preferred fluors and your interest in performing
multi-target detection.
• Determine the degree of amplification you need, taking into account the abundance of the protein
or gene in your specimen and your need to conserve antibody or probe.
Then, based on your experience, choose one of three TSA formats:
• Complete TSA Systems: complete tyramide signal amplification kits include labeled tyramide, amplification
diluent, streptavidin HRP, blocking reagent and comprehensive instruction manuals. Additional antibodies,
conjugates, and chromogens may be required for your optimal protocol and may be purchased separately.
• Complete TSA Plus Systems: tyramide signal amplification kits that provide 10 to 20 times the
sensitivity enhancement of regular TSA systems.
• TSA Reagent Packs: stand-alone amplification reagents that provide researchers experienced
with TSA the flexibility to develop their own protocols.
Mouse Brain, 20x magnification, 15 second exposure
Conventional detection with
fluorescein conjugated 2° antibody.
Dilution 1:100
12
Standard TSA fluorescein.
Dilution 1:10,000
TSA Plus fluorescein.
Dilution 1:1,000,000
for your laboratory…
Complete TSA Systems
TSA Fluorescence Systems
• Used for direct fluorescence detection in IHC and
ISH applications.
• While not as sensitive as TSA Plus systems, allow
significant reductions in antibody or probe with
necessary sensitivity in many applications.
A
TSA Biotin Systems
• Designed for chromogenic detection in ISH and
IHC applications.
• Can also be used for indirect fluorescence detection
to maximize fluorescent signal amplification.
• Use streptavidin-conjugated chromogens or
fluorophores for visualization.
B
IHC detection of CD3 antigen in serial sections of paraffinembedded human tonsil. (A) Rabbit anti-CD3 1:400, biotinylated
anti-rabbit, ABC, with DAB chromogenic detection. (B) TSA
Biotin: Rabbit anti-CD3 1:400, biotinylated anti-rabbit, SA-HRP,
biotinyl tyramide, ABC, with DAB chromogenic detection.
(Courtesy of F. van den Berg and A. de Koning, Dept. Pathology,
AMC, Amsterdam, The Netherlands.)
Complete TSA Plus Systems
TSA Plus Fluorescence Systems
• Used for direct fluorescence detection in IHC and
ISH applications.
• Provide very high sensitivity in ISH compared to
traditional methods.
• While developed specifically for ISH applications,
these systems can also be used with IHC applications.
Embryonic mouse
brain. Histone H4
mRNA ISH, using
TSA Plus Cyanine 3
and MAP2
immunoreactivity
(IHC) with TSA Plus
Fluorescein System.
• Choose from five different fluorphores: fluorescein,
TMR, coumarin, Cyanine-3, or Cyanine-5.
TSA Plus Fluorescence Palette Systems
• Convenient and cost-effective way of combining
two fluors for multi-target detection procedures.
• Choose from four different two-fluor combinations.
• Evaluation of TSA fluorescence detection or exploring
multi-target detection is easy with our TSA Plus
Fluorescence Palette System, a trial-sized kit with
four different fluors packaged together. Each fluor
has sufficient reagent for 10 –35 slides.
Multi-target detection
in the same sample.
Detection of ArgVasopressin and Oxytocin
in rat brain using TSA
Cyanine 3 and TSA
Cyanine 5, respectively.
w w w. p e r k i n e l m e r. c o m
13
…with the flexibility to match your
research and application needs.
TSA Plus DNP Systems
TSA Reagent Packs
• Designed for biotin-free chromogenic detection
in IHC and ISH applications.
TSA Reagent Packs are stand-alone tyramide (SAT)
kits for signal amplification that provide flexibility
to researchers for TSA protocol development.
Researchers must supply their own blocking buffer,
HRP and other reagents as needed. If you have not
used TSA Signal Amplification reagents before, we
strongly suggest purchasing one of our complete TSA
systems, which include a set of quality controlled
reagents optimized to work well together.
• Use to reduce background associated with
endogenous biotin.
• Simple protocol eliminates biotin-quenching
procedures.
• The ISH protocol included is specifically optimized
for using with popular DIG-labeled probes,
although any hapten-labeled probe can be used.
• Enhances sensitivity of existing DIG labeling protocols.
Use versatile, flexible TSA to enhance
sensitivity in many applications
TSA is compatible with all common
detection methods:
TSA makes a difference in the sensitivity of any
application using an antibody or gene probe that
allows the addition of HRP into its protocol:
• Enzymatic
ABC (avidin-biotinylated HRP complex)
• ELISA: amplify signal in microplate-based assays,
greatly increasing sensitivity or reducing the use of
antibodies. Use our convenient ELAST ® ELISA
Amplification System.
• Protein microarrays: detect low abundance proteins
in differential protein expression experiments. Use
our TSA AUTOMATED Kits with our ProteinArray™
Workstation, a highly flexible, fully automated system for processing multiple protein microarrays.
• Genomic microarrays: apply TSA to your cDNA
differential gene expression studies using our
MICROMAX™ TSA Labeling and Detection Kits.
PAP (peroxidase anti-peroxidase)
APAP (alkaline phosphatase anti-alkaline
phosphatase)
• Chromogenic
BCIP/NBT (using alkaline phosphataseconjugated secondary antibody or avidin)
DAB (using second application of HRPconjugated secondary antibody or avidin)
• Electron Microscopy (EM)
Gold conjugate
Immunogold detection systems
• Fluorescence (Direct and Indirect)
Quick Glance
Expert technical support with one phone call
PerkinElmer Life and Analytical Sciences gives you easy access to a worldwide network of local
support offices, staffed by highly trained professionals who speak your language. You’ll get expert
assistance for all of your questions — no matter where you are located — when you add TSA to
your protocols. Contact us at (800) 762-4000 or (+1) 203-925-4602, or by e-mail at
techsupport@perkinelmer.com or techsupport.europe@perkinelmer.com.
14
Ordering Information
TSA Fluorescence Systems
Product
No. of Slides
Product No.
TSA Fluorescein System
50–150
100–300
100–300
100–300
50–150
50–150
NEL701A001KT
NEL701001KT
NEL702001001KT
NEL703001KT
NEL704A001KT
NEL705A001KT
TSA TMR System
TSA Coumarin System
TSA Cyanine 3 System
TSA Cyanine 5 System*
TSA Biotin Systems
Product
No. of Slides
Product No.
TSA Biotin System
50–150
200–600
NEL700A001KT
NEL700001KT
Product
No. of Slides
Product No.
TSA Plus Fluorescein System
50–150
250–750
50–150
250–750
50–150
250–750
50–150
250–750
NEL741001KT
NEL741B001KT
NEL742001KT
NEL742B001KT
NEL744001KT
NEL744B001KT
NEL745001KT
NEL745B001KT
Product
No. of Slides
Product No.
TSA Plus Cyanine 3/Cyanine 5 System*
TSA Plus Cyanine 3/Fluorescein System
TSA Plus Cyanine 5/Fluorescein System*
TSA Plus Fluorescein/TMR System
TSA Plus Fluorescence Palette System (contains one each of Fluorescein, TMR, Cyanine 3 and Cyanine 5)*
50–150
50–150
50–150
50–150
10–35
NEL752001KT
NEL753001KT
NEL754001KT
NEL756001KT
NEL760001KT
Product
No. of Slides
Product No.
TSA Plus DNP (AP) System
25–75
50–150
25–75
50–150
NEL746B001KT
NEL746A001KT
NEL747B001KT
NEL747A001KT
Product
No. of Slides
Product No.
TSA Biotin Tyramide Reagent Pack
200–600
1,000–3,000
100–300
500–1,500
100–300
50–150
250–750
50–150
SAT700001EA
SAT700B001EA
SAT701001EA
SAT701B001EA
SAT702001EA
SAT704A001EA
SAT704B001EA
SAT705A001EA
TSA Plus Fluorescence Systems
TSA Plus TMR System
TSA Plus Cyanine 3 System
TSA Plus Cyanine 5 System*
TSA Plus Fluorescence Palette Systems
TSA Plus DNP Systems (Biotin-free)
TSA Plus DNP (HRP) System
TSA Reagent Packs
TSA Fluorescein Tyramide Reagent Pack
TSA TMR Tyramide Reagent Pack
TSA Cyanine 3 Tyramide Reagent Pack
TSA Cyanine 5 Tyramide Reagent Pack*
*Cyanine 5 tyramides cannot be visualized with most digital cameras.
w w w. p e r k i n e l m e r. c o m
15
Complementary Products for Use with TSA
These high quality conjugates, chromogens and secondary antibodies can be used with TSA Assay Systems and Reagent Packs.
Chromogens & Conjugates
Product
Product No.
BCIP/NBT (AP substrate)
DAB (HRP substrate)
Antifluorescein-AP
Antifluorescein-HRP
Streptavidin Fluorescein
Streptavidin Texas Red®
Streptavidin Coumarin
Streptavidin-HRP
Streptavidin-AP
NEL937001PK
NEL938001EA
NEF709001PK
NEF710001EA
NEL720001EA
NEL721001EA
NEL722001EA
NEL750001EA
NEL751001EA
Secondary Antibodies
Antibody
Label
Size
Concentration
Product No.
Anti-human IgG (goat)*
Anti-human IgG (goat)*
Anti-human IgG (goat)
Anti-mouse IgG (goat)
Anti-mouse IgG (goat)
Anti-mouse IgG (goat)
Anti-rabbit IgG (goat)
Anti-rabbit IgG (goat)
Anti-rabbit IgG (goat)
AP
HRP
Biotin
AP
HRP
Biotin
AP
HRP
Biotin
1 mL
1 mL
0.5 mg
1 mL
1 mL
0.5 mg
1 mL
1 mL
0.5 mg
1 mg/mL
1 mg/mL
Lyophilized
1 mg/mL
1 mg/mL
Lyophilized
1 mg/mL
1 mg/mL
Lyophilized
NEF801001EA
NEF802001EA
NEF803001EA
NEF821001EA
NEF822001EA
NEF823001EA
NEF811001EA
NEF812001EA
NEF813001EA
*Bovine serum albumin added as a stabilizer.
TSA technology from PerkinElmer —
the difference is clear!
Add TSA to your protocols and you, too, will see the difference. Learn more about the
exciting enhancements you’ll see when you use the ultimate technology for maximizing
detection sensitivity. For more information, visit www.perkinelmer.com/tsa, phone
(800) 762-4000 or (+1) 203-925-4602, or contact your local sales representative. For a
complete listing of our global offices, visit www.perkinelmer.com/lasoffices.
PerkinElmer Life and
Analytical Sciences
710 Bridgeport Avenue
Shelton, CT 06484-4794 USA
Phone: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com
For a complete listing of our global offices, visit www.perkinelmer.com/lasoffices
©2007 PerkinElmer, Inc. All rights reserved. The PerkinElmer logo and design are registered trademarks of PerkinElmer, Inc. TSA, ProteinArray, MICROMAX and ELAST are
trademarks or registered trademarks of PerkinElmer, Inc. or its subsidiaries, in the United States and other countries. Texas Red is a registered trademark of Teletype Corp.
All other trademarks not owned by PerkinElmer, Inc. that are depicted herein are the property of their respective owners. PerkinElmer reserves the right to change this
document at any time without notice and disclaims liability for editorial, pictorial or typographical errors.
007703_01
Printed in USA