Date
Week 1
May
26
Professor Lester’s BIOL 210 Summer 2015 Lab Syllabus*
Lab Exercises
Microscope; Environmental plate; Lab Basics
Chapter
3-1, 2-1
27
Sterile technique; smears; Simple & Gram stains
1-4, 3-4, 3-6
28
June
Week 2
1
Acid-fast and Spore stains; continue Gram stain
3-7, 3-9
2
Environmental plate cont.; Negative stain, Motility; Finish Gram Stain
(turn in slide for grade)
Growth Patterns; Practice staining
Lab Quiz 1
3
Live Protozoa; Parasitic Protozoa; Micropipettor practice
Handout from web site
4
Morphological Unknown
Handout from web site
Week 3
8
Due Same Day
Media: Selective&Differential; Temperature and Oxygen; Streak Plate
(streak plate will be graded)
9
Transformation; Standard plate count
10
Finish all labs; Review for practical
11
Lab Practical 1
Week 4
15
16
18
Water Quality: Membrane Filter; ; Gradient Plate;
cont. unknowns
Week 5
22
4-1, 4-3, 4-4, 4-5; 2-6,
2-7, 2-8;1-5
Lab Quiz 2
Metabolism: Going over many results!
Begin Unknown #2 – staining and streaking
Chemotherapeutics: disinfectants, antiseptics; Antibiotics;
continue unknowns – Enterotube inoculation
2-3, 2-4
8-3, 6-1
Metabolism: Catalase, Nitrate, Starch, Lipid, Protein; Carbs ,Indole, MR-VP,
citrate, H2S, Urease
17
3-5, 3-8, 3-10, 5-24
5-2,3,4,6,7,11,
14,16
Handout
2-13, 7-2
Lab Quiz 3
Finish water; Viruses;
DNA Sequencing Pt.1 – DNA extraction and PCR; continue unknowns
23
DNA Sequencing Pt. 2 – Electrophoresis ; Snyder test for Dental Caries;
Begin Throat&Skin microbes; Fungi; continue unknowns
24
UV; Quorum Sensing Video; continue unknowns
25
cont. Throat&Skin microbes (coagulase, novobiocin)
Unknown #2 Due; DNA Sequencing Pt.3 – Identify unknown
7-5; web handout
6-3
DNA Handout
DNA Handout, 7-1,
5-21, 5-23; see 3-3
8-2
Lab Quiz 4
5-21, 5-23
Week 6
29
Complete all labs; Discussion or Activity; review for practical
30
Lab Practical 2
*This syllabus is tentative and subject to change.
DNA Handout