CLAUSEN MEDIUM
Product Number C0596
Product Description
Clausen Medium is recommended for sterility testing.
This medium is a very nutritious medium consisting of
casein enzymic hydrolysate, papaic digest of soyabean
meal, yeast extract and dextrose. L-cystine and
thioglycollate act as reducing agents. The metals help
isolate anaerobic sporeformers. Tween 80 is added to
this medium to overcome the effects of cationic agents
(in combination with lecithin already present in the
formulation) which can exert a bacteriostatic effect in
vitro.
This medium is yellow colored and clear in appearance.
Under aerobic conditions it turns pink. Therefore at the
time of use the upper third of the medium should be
pink.
Components
Item
Casein Enzymic Hydrolysate
Papaic Digest of Soyabean Meal
Yeast Extract
Dextrose
Sodium Chloride
Dipotassium Phosphate
Sodium Citrate
L-Cystine
L-Asparagine
Sodium Dithionite
Sodium Thioglycollate
Lecithin
Magnesium Sulfate
Calcium Chloride
Cobalt Sulfate
Cupric Sulfate
Ferrous Sulfate
Zinc Sulfate
Manganese Chloride
Resazurin
Agar
Final pH (at 25 °C) 7.1 ± 0.2
g/L
15.00
3.00
6.00
6.00
2.50
2.00
1.00
0.50
1.25
0.40
0.50
0.30
0.40
0.004
0.001
0.001
0.001
0.001
0.002
0.001
0.75
Precautions and Disclaimer
For laboratory use only. Not for drug, household or
other uses.
Preparation Instructions
Suspend 40 grams of Clausen Medium in 1000 mls of
distilled water containing 3 grams of (P 8074) Tween 80
and 5 grams of (G 6279) Glycerol. Heat to boiling to
dissolve the medium completely. Dispense as desired
and sterilize by autoclaving at 15 lbs. pressure (121 °C)
for 15 minutes. Place in a cool, dark place until use. Do
not resterilize the medium.
Storage
Store the dehydrated medium at 24 °C. The prepared
medium should be stored in a cool place (NOT 4 °C)
and in the dark for not longer than one month.
Product Profile
Appearance
Yellow colored, homogeneous,
free flowing powder.
Color and Clarity
Light straw colored, clear to
slightly opalescent solution with
the upper 10% of the solution
pink upon standing.
Cultural Response
Cultural characteristics are
observed after 18-48 hours at 35
– 37 °C.
Organism
Bacillus subtilis
Candida albicans
Clostridium sporogenes
Pseudomonas aeruginosa
Staphylococcus aureus
Staphylococcus epidermidis
Streptococcus pyogenes
(ATCC)
(6633)
(10231)
(11437)
(27853)
(25923)
(12228)
(19615)
Growth
luxuriant
luxuriant
luxuriant
luxuriant
luxuriant
luxuriant
luxuriant
References
1. Clausen, O.G., (1973). Pharmaceutica Acta
Helvetiae. 48, 541.
2. American Type Culture Collection, Manassas, Va.,
U.S.A..
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser
must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of
the invoice or packing slip.