ekspert™ nanoLC 400 Systems
Site Planning Guide
RUO-IDV-01-1234-A
May 2014
This document is provided to customers who have purchased AB SCIEX equipment to use in the
operation of such AB SCIEX equipment. This document is copyright protected and any
reproduction of this document or any part of this document is strictly prohibited, except as
AB SCIEX may authorize in writing.
Software that may be described in this document is furnished under a license agreement. It is
against the law to copy, modify, or distribute the software on any medium, except as specifically
allowed in the license agreement. Furthermore, the license agreement may prohibit the software
from being disassembled, reverse engineered, or decompiled for any purpose. Warranties are as
stated therein.
Portions of this document may make reference to other manufacturers and/or their products,
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trademarks of their respective owners. Any such use is intended only to designate those
manufacturers' products as supplied by AB SCIEX for incorporation into its equipment and does
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including without limitation, warranties of merchantability or fitness for a particular purpose,
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all of which are expressly disclaimed, and assumes no responsibility or contingent liability,
including indirect or consequential damages, for any use by the purchaser or for any adverse
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For research use only. Not for use in diagnostic procedures.
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owners.
AB SCIEX™ is being used under license.
© 2014 AB Sciex Pte. Ltd.
Printed in USA.
AB Sciex Pte. Ltd.
Blk 33, #04-06
Marsiling Ind Estate Road 3
Woodlands Central Indus. Estate
SINGAPORE 739256
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Contents
Chapter 1 Customer and FSE Responsibilities. . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Customer Site Planner Responsibilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
FSE Responsibilities . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
During Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
Operator Familiarization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
Technical Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
Chapter 2 Site Planning Checklist . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Customer Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Comments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Exceptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Signoff . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
Appendix A Site Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Environmental Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Physical Layout Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Physical Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11
Recommended Bench Space . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
AC Mains Supply Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Power Consumption . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
System Electrical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Protective Earth Conductor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
AC Mains Supply Connections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
UPS (Uninterruptible Power Supply) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Gas Supply Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Ventilation and Waste Collection Requirements . . . . . . . . . . . . . . . . . . . . . . . . .15
Laboratory Ventilation Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
Network and Computer Equipment Requirements . . . . . . . . . . . . . . . . . . . . . . .16
Network Connection (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Customer Supplied Solutions and Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Appendix B Equipment Safety Categories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17
Revision History. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
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Contents
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1
Customer and FSE Responsibilities
This guide is for the site planner, the individual responsible for preparing the facility for the
installation of the Eksigent ekspert™ nanoLC 415 or 425 system. For site planning information for
the mass spectrometer, refer to the appropriate instrument Site Planning Guide.
Note: If the site preparation tasks are not complete when the AB Sciex Field Service
Employee (FSE) arrives, the scheduled installation will be postponed.
Customer Site Planner Responsibilities
Complete the checklist in Site Planning Checklist in consultation with facilities services personnel
(gas, electrical, ventilation, and IT), and return it to the FSE before the completion date. Refer to
Signoff on page 9.
Note: The FSE will follow up if the checklist is not received prior to the scheduled
installation date.
•
Carefully inspect the boxes and note any damage or mishandling on the shipping
documents. Contact the manufacturer if the boxes were mishandled during transit.
After all requirements are met, contact a customer service representative to
schedule installation. (Tel: 1 877-740-2129 Email: CustomerCare@absciex.com)
•
Verify that the requirements for the operating environment are met. See
Environmental Requirements on page 11.
•
Provide all required electrical receptacles. See AC Mains Supply Requirements on
page 12.
•
Provide all required regulators, shut off valves, and gases. See Gas Supply
Requirements on page 14.
•
Provide and install all required vents and ventilation devices. See Ventilation and
Waste Collection Requirements on page 15.
•
Select the desired configuration, and verify that adequate space is available. See
Physical Layout Requirements on page 11.
•
Provide a printer and (optional) active, tested LAN connection. See Network and
Computer Equipment Requirements on page 16.
•
Verify that all required solvents and laboratory supplies are available. See Customer
Supplied Solutions and Equipment on page 16.
FSE Responsibilities
•
Review the checklist and discuss outstanding issues with the site planner.
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Customer and FSE Responsibilities
•
Supply all fittings, plugs, and cables required to connect the device to the
recommended electrical receptacles, provided they are within the maximum
distances described later in this guide.
•
Test and qualify the system to the specifications in the Installation Checklist and
Data Log.
•
Set up LC equipment sold and supported by AB Sciex. Integrate other supported LC
equipment.
•
Set up optional gas generator equipment sold by AB Sciex or Eksigent.
During Installation
After an FSE unpacks the system (with assistance from people at your site), it takes several
hours for the FSE to set up the system and verify its operation. Then, the FSE will perform
installation qualification tests.
Operator Familiarization
During installation, the FSE provides a system and software overview, reviews data, and
provides some basic operator familiarization using the Customer Familiarization Checklist.
Training for auxiliary equipment supplied by other manufacturers is not provided by the FSE.
Note: Online training is available at www.absciex.com/LearningPortal.
Technical Support
AB Sciex and its representatives maintain a staff of fully-trained service and technical specialists
located throughout the world. They can answer questions about the system or any technical
issues that may arise. For more information, visit the web site at www.absciex.com.
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2
Site Planning Checklist
Customer Information
Organization
Address
City
Country
ZIP/Postal code
Telephone #
Fax #
Site planner contact
name
E-mail address
Requirements
Complete the checklists in this section to ensure the installation site is ready for installation. For
detailed information, refer to Appendix A: Site Requirements.
Table 2-1
Environmental Requirements Checklist
Requirements
Complete
The ambient temperature is 18°C to 30°C ±2° (64°F to 86°F ±3.6°).
Note: Peripheral devices may have different operating environment
requirements. Check the operating environment requirements with the
vendor of each peripheral device that will be used with the mass
spectrometer.
Relative humidity is 20% to 80%, non-condensing.
Table 2-2
Electrical Requirements Checklist
Requirements
Complete
Branch circuits are terminated with NEMA 6-15R or local, standard
receptacles. Each branch circuit must have two outlets. (North America
only)
Computer equipment (computer, monitor, printer): minimum of four mains
supply outlets. North America:120 VAC, 60 Hz. Europe: 230 VAC, 50 Hz;
Japan: 100 VAC, 50/60 Hz.
Up to two additional mains required for the cHiPLC® system and ion source,
when present.
International
Installation adheres to local requirements.
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Site Planning Checklist
Table 2-3
Gas Supply Requirements Checklist
Requirements
Complete
Clean, dry air or nitrogen
Delivery pressure of 100 psi (6.9 bar)
Table 2-4
Ventilation and Waste Collection Requirements Checklist
Requirements
Complete
Installation of plumbing and ventilation fixtures complies with local bylaws
and biohazard regulations.
Table 2-5
Computer Equipment Requirements Checklist
Requirements
Complete
The network or IT specialist is available to install the security software while
the FSE is present.
A network printer or a dedicated printer is available, along with any
necessary print drivers.
A computer table is available for the data acquisition workstation, within 2 m
(6 ft) of the system.
Table 2-6
Network Equipment Requirements Checklist
Requirements
Complete
An active, tested LAN connection is available.
A computer name has been defined for the acquisition computer on the
appropriate domain.
The network or IT specialist is available to connect the computer to the LAN
while the FSE is present.
Table 2-7
Physical Layout Requirements Checklist
Requirements
Complete
There is adequate lab space to accommodate the equipment.
Table 2-8
General Laboratory Requirements Checklist
Requirements
Complete
Required pipettors are available such as 2 µL, 10 µL, 20 µL, 100 µL or
200 µL and 1 mL.
Required solutions are available:
• HPLC-grade or MS-grade methanol (1 L)
• HPLC-grade or MS-grade acetonitrile (1 L)
• HPLC-grade deionized water (1 L)
• Highest purity formic acid (a 1 mL ampule is sufficient for a standard
month of runs)
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Site Planning Checklist
Comments
Exceptions
Signoff
Complete the checklist and return it to the FSE email specified below on or before the Return
date.
Site planner name
Site planning return
completion date
(yyyy-mm-dd)
I, the Site Planner, acknowledge that all installation requirements, as specified in
this document, have been met.
FSE name
Return date
(yyyy-mm-dd)
FSE email
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Site Planning Checklist
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A
Site Requirements
This appendix describes the site requirements for the Eksigent ekspert™ nanoLC 415 or 425
pump and the ekspert nanoLC 400 autosampler.
Environmental Requirements
The following are standard requirements:
•
Ambient temperature of 18°C to 30°C ±2°C (64°F to 86°F ±3.6°F)
•
Relative humidity from 20% to 80%, non-condensing
Note: Operation of the device at altitudes higher than 2000 m (6400 ft) above sea level
might impact device performance.
Physical Layout Requirements
WARNING! Electrical Shock Hazard: Do not block the AC mains power
connection. Potential risk of personal injury or equipment damage if the system
cannot be turned off or disconnected from the mains supply outlet in an
emergency. Position the system so that the mains switch and outlet can be easily
accessed.
Physical Dimensions
Examine the weight and dimensions in Table A-1 and make sure that you can move the system
to the installation site. Make sure that the installation site can accommodate the equipment
dimensions and weight.
Table A-1
Weights and Dimensions
Equipment
Height
Width
Depth
Weight
ekspert nanoLC
pump
23 cm (9 inches)
56 cm
(22 inches)
66 cm
(26 inches)
26 kg (57 lbs)
ekspert nanoLC 400 49 cm (19 inches) 33 cm
autosampler
(13 inches)
66 cm
(26 inches)
15 kg (33 lbs)
Autosampler solvent 9 cm (3.5 inches) 33 cm
tray
(13 inches)
36 cm
2 kg (4.5 lbs)
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(14 inches)
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Site Requirements
Recommended Bench Space
For the system, we recommend a minimum bench footprint of 56 cm (22 inches) wide x 71 cm
(28 inches) deep. When the autosampler is stacked on top of the nanoLC pumps, we
recommend a minimum height allowance of 102 cm (40 inches).
The bench must support up to 70 kg (154 lbs) including the autosampler.
Laboratory Layout
Figure A-1 shows a suggested laboratory layout for the ekspert nanoLC 400 system.
Figure A-1 Laboratory Layout
Mains Supply
Wall
2 m max
1.6 m max
LC System
2 m max
Roughing
Pump
1 m min
Gas
Generator
Mass Spectrometer
and Ion Source
2 m max
Computer
AC Mains Supply Requirements
WARNING! Electrical Shock Hazard: Use only qualified personnel for the
installation of all electrical supplies and fixtures, and make sure that all
installations adhere to local bylaws.
Power Consumption
System
Power Consumption
ekspert nanoLC 415 and 425 pumps
240 W
ekspert nanoLC 400 autosampler
240 W
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Site Requirements
System Electrical Specifications
Note: Specifications are subject to change without notice. Contact your FSE for the
latest specifications.
Table A-2
Pump Electrical Specifications
Specification
Value
Nominal input voltage (device)
24 VDC / 5 VDC
Nominal input voltage (power supply)
100 to 125 VAC / 200 to 240 VAC
Frequency
50/60 Hz
Max rated input current
2 AAC / 4 AAC
Table A-3
Autosampler Electrical Specifications
Specification
Value
Nominal input voltage
100 VAC to 240 VAC
Frequency
50/60 Hz
Max rated input current
4 AAC
Protective Earth Conductor
WARNING! Electrical Shock Hazard: Do not intentionally interrupt the protective
earth conductor. Any interruption of the protective earth conductor is likely to
make the installation dangerous.
The AC mains supply should include a correctly installed protective earth conductor. A qualified
electrician must install or inspect the earth conductor before the device is connected.
AC Mains Supply Connections
Note: The mains supply outlets must be within 2 m (6 ft) of the Eksigent system.
North America Requirements
•
Up to six 100/240 VAC receptacles for the nanoLC pump, autosampler, cHiPLC®
system, computer, monitor, and ion source.
International Requirements
For installations outside North America, use locally approved standard connections and cables.
•
Europe: 230 VAC, 50 Hz
•
Japan: 100 VAC, 50/60 Hz
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Site Requirements
UPS (Uninterruptible Power Supply)
A pure sine-wave UPS or power conditioner can be used to maintain the system supply during
power outages. If the voltage changes more than ten percent in 24 hours, use a power
conditioner.
The UPS or power conditioner that you use to power the system must meet the specifications in
Table A-4.
Table A-4
UPS and Power Conditioner Requirements
Specification
Requirements
Output voltage
100 VAC to 125 VAC
200 VAC to 240 VAC true on line (double conversion)
Frequency
50 Hz/60 Hz
Waveform
Pure sine wave
Minimum peak current
3 × nominal current
Output voltage distortion
< 3%
Output protection
Circuit breaker
Note: We do not supply an external UPS or power conditioner for the device, and the
FSE is not responsible for configuring or connecting a UPS or power conditioner.
Note: Capacity required varies depending on the components connected. Check the
requirements for the individual components.
Gas Supply Requirements
Caution: Potential Instrument Damage: Only use approved gas generators, regulators,
and connectors. See Table A-7.
Tip! To save space, gas supplies may be installed outside the laboratory, as long as
the required outlet pressures and flow rates can be maintained.
Table A-5
Gas Pressure
Gas
Rating
Air or nitrogen
100 psi
* 25 ft of flexible plastic 1/4 o.d. inch tubing is supplied. Make sure the appropriate fittings and
adapters for connection to the regulator are supplied.
Table A-6
Gas Rate Consumption
Model
Rating
nanoLC 415 system
0.795 liters per hour
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Site Requirements
Table A-6
Gas Rate Consumption (cont’d)
Model
Rating
nanoLC 425 system
1.325 liters per hour
Note: Operation of the system requires connection to a source of 100 psi (6.9 bar)
regulated clean, dry air or nitrogen. The system should be within 6 m (20 ft) of the air/
nitrogen regulator. When using compressed air, we recommend an air supply with a
dew point of less than 4.5°C (40°F).
For compressed gas supplied at less than 150 psi, we recommend the use of air
filtration to 5 µm via a Wilkerson F18 filter and regulation to a working pressure of 100
psi via an oiled compressor.
We recommend the regulator be followed with a coalescing filter and a Wilkerson R18
regulator. For compressed gas supplied at less than 150 psi, you may also use a
Wilkerson R18 regulator or a filter suitable for particle removal to 0.01 µm (Wilkerson
M18 coalescing filter).
Table A-7
Required Gas, Regulators, and Fittings
Gas
Regulators
Fitting
Size
Suggested
Cylinder/ Dewar
Nanufacturer Gas Regulator
PN
Air or
nitrogen
Maximum 100
psi (6.9 bar)
1/4 inch
Matheson
House Gas
Regulator
PN
SP-3810-590 (air) or SP-3231
SP-3810-580 (N2)
Ventilation and Waste Collection Requirements
WARNING! Toxic Chemical Hazard: Use only qualified personnel for the
installation of plumbing and ventilation fixtures. Make sure that all installations
follow local bylaws and biohazard regulations.
WARNING! Toxic Chemical Hazard: Use the source exhaust system to remove
sample vapor exhaust pump exhaust from the laboratory environment. The lab
ventilation system integrity is vital for operator safety.
Laboratory Ventilation Device
•
Average airflow velocity of 30 linear m/min (100 linear ft/min). The actual amount of
airflow rate depends on the cross sectional area of the duct and can be calculated
from this formula:
Airflow rate = Velocity × Area (cross sectional area of the duct)
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Site Requirements
Network and Computer Equipment Requirements
•
Have a network or IT specialist available to install the security software while the
FSE is present.
•
Provide a network or dedicated printer, along with any necessary print drivers.
•
Provide a table for the data acquisition workstation, within 2 m (6 ft) of the device.
•
Provide a computer that meets these requirements:
•
Windows XP / Windows 7 operating system.
•
Re-writable CD-ROM drive.
•
2.8 GHz Pentium 4 processor; 512 Mb RAM; 40 Gb hard disk drive.
•
2 USB ports.
Network Connection (Optional)
•
Provide an active, tested LAN connection for the acquisition computer.
•
Define a computer name for the acquisition computer on the appropriate domain.
•
Have a network or IT specialist available to connect the computer to your LAN while
the FSE is present.
Customer Supplied Solutions and Equipment
WARNING! Toxic Chemical Hazard: Follow all safety guidelines when handling,
storing, and disposing of chemicals. For detailed safety instructions, refer to the
System User Guide.
For best results, use high purity solvents for nanoflow experiments. Low quality solvents may
result in high background, contaminant peaks, or clogging of parts in the LC systems.
Contaminants are difficult to remove from the LC system, and may result in downtime or require
a billable service call.
Tip! AB SCIEX recommends pre-made, high purity solvents such as those from
Burdick-Jackson. These solvents can be ordered from VWR:
- PN BJLC452-1.0 - 0.1% Formic Acid#Water (HPLC-grade water with 0.1% formic acid)
- PN BJLC441-1.0 - 0.1% Formic Acid#Acetonitrile (acetonitrile with 0.1% formic acid)
•
Pipettors (2 µL, 10 µL, 20 µL, 100 or 200 µL, and 1 mL) and appropriate tips
•
HPLC vials with conical bottom (Eksigent autosampler vials) (a small quantity is
supplied with the system)
•
HPLC-grade or MS-grade methanol (1 L)
•
HPLC-grade or MS-grade acetonitrile (1 L)
•
HPLC-grade deionized water (1 L)
•
Highest purity formic acid (a 1 mL ampule is sufficient for a standard month of runs)
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Equipment Safety Categories
Table B-1
B
Equipment Safety Categories
Description
Category
Equipment pollution degree
Pollution Degree 2
Mains supply transient overvoltage Overvoltage Category II
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Equipment Safety Categories
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Revision History
Revision
Reason for Change
D5033471 A
This is the first release of the Site Planning Guide.
D5033471 B
Added Equipment Safety Categories appendix.
D5033471 C
RUO-IDV-01-1234-A
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Updated document template. Updated Figure A-1.
Increased number of outlets required.
Date
September 2012
January 2013
May 2014
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Revision History
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