Curriculum Vitae Jihong Liu Clarke Curriculum Vitae 1. Personal information Jihong Liu Clarke, PhD Senior research scientist Female Norwegian Chinese (Mother tongue), English and Norwegian Married Orkerødgata 66B, 1511 Moss, Norway Bioforsk, Høgskoleveien 7, 1430 Ås, Norway Tel. +47-99594790 E-mail: jihong.liu-clarke@bioforsk.no. Name Title Gender Nationality Languages Civil status Home address Work address 2. Education and scientific degrees 1997-2003 PhD, Pathology & Molecular Biology, Norwegian Univ. Life Sci., Aas, Norway 1992-1995 Licentiate Phil, Plant Biotechnology, Swed. Univ. Agric. Sci., Uppsala, Sweden 1983-1986 MSc, Genetics, Sichuan Agricultural University, Sichuan, China 1979-1983 BSc and Diploma, Agronomy, Jilin Agricultural University, Changchun, China 3. Employment 2006- present 2005- present 2003-2005 1990-1991 1986-1990 Coordinator for China collaboration at Bioforsk Permanent research scientist, Bioforsk, Ås, Norway Post doc, Norwegian Crop Research Institute, Ås, Norway Lecturer in plant genetics, Sichuan Agricultural University, China Assistant Lecturer in genetics, Sichuan Agricultural University, China 4. Scientific awards Innovation prize winner (2nd prize), Bioforsk 2006 5. Patent 2007 UK patent on Agrobacterium-mediated transformation methodology for poinsettia 6. Key qualifications Scientifically: I have over 15 years’ experience in tissue culture and plant biotechnology of various plants including oilseed Brassica napus, barley, tobacco and poinsettia with focus on genetic engineering of ornamental crops and molecular farming for plant produced human and fish vaccines as well as industrial enzymes. 1 Curriculum Vitae Jihong Liu Clarke Project management: I have been project manager since 2005 on various projects. Research for innovation: have a UK patent. Supervision: I have been supervisor or co-supervisor for 5 PhD students and 5 master students. Editorial and guest editor: I am assigned as an editorial board member for three journals and served as a guest editor for two special issues and is working on the 3rd special issue. Coordination and international relations: I have been the coordinator for China relations at Bioforsk since 2006 and have a strong network with colleagues across Asia, Europe and USA. Program board: A member of expert advisory group for CHINOR program, Research Council of Norway. 7. Editorial positions Editorial board member of Plant Biotechnology Journal (Impact factor 20136.279 in 2012) Guest editor for Plant Biotechnology Journal Special Issue on 2013-2014 Biotechnology for biorefinery Guest editor for Plant Molecular Biology Special Issue on Plant 2012-2013 Biotechnology for Bioeconomy Guest editor for Plant Molecular Biology Special Issue on Chloroplast 2011-2012 Evolution and Biotechnology Editorial board for journal “GM Crops and Food 2010Editorial board for journal “Soil & Plant Science, Acta Agriculture 2009Scandi, Section B” 8. Professional membership Appointed by the Research Council of Norway as a member of “Expert 2011-2014 Advisory Group for the CHINOR programme (bilateral program between Norway and China) Member of Norwegian Scientific Committee for Food Safety (VKM), 2007-2009 Group 3 for evaluation of GMO applications Member of American Society for In Vitro Biology 2007-2009 9. Co-supervisor to 5 PhD students 2009-2014 Johanna Gottschamel. A joint PhD student at University of Natural Resources and Applied Life Sciences, Vienna, Austria) on tobacco chloroplast-derived dengue vaccine. I am a co-supervisor 2011-2014 Zhibo Zhang. A joint PhD student between Northwest Agriculture and Forest University, Shaanxi China and Bioforsk. I am a co-supervisor. 2009-2013 Ashraf Islam. Norwegian University of Life Sciences and Bioforsk, Ås, Norway. Received his PhD degree on June 19, 2013 with the thesis on “Genetic engineering and light quality as tools to control shoot elongation in poinsettia (Euphorbia pulcherrima 2 Curriculum Vitae Jihong Liu Clarke 2010-2012 2010-2011 willd ex Klotzsch)”. I was responsible for most of the PhD project work Chuan Li: A Chinese PhD student from Sichuan Agricultural University. I am functioning as an external supervisor. Xiaoli Xiang. A Chinese PhD student from Sichuan Agricultural University. I am functioning as an external supervisor for partial of her PhD work during her stay in Norway 10. Co-supervisor to 5 master students 2012-2013 Camilla Østerud. Norwegian University of Life Sciences and Bioforsk 2011-2012 Maria Blø Kirkeland. Norwegian University of Life Sciences and Bioforsk 2011-2012 Kine Gjørvad. Norwegian University of Life Sciences and Bioforsk 2009-2011 Shuai Guo. Norwegian University of Life Sciences and Bioforsk 2006-2007 Merete Wiken Dees. Norwegian University of Life Sciences and Bioforsk 11. Project manager experiences and selected projects 2015-2018 ERA-NET: PlantVac Sustainable management of aquaculture fish health through the development of low cost plant-derived vaccines, using vaccines against salmon pancreas disease and viral haemorrhagic septicaemia as case studies 2014-2017 EEA Norway-Romania research project: DEVELOPMENT OF A COST EFFECTIVE ROMANIA-NORWAY JOINT PLANT-BASED TECHNOLOGY PLATFORM FOR PRODUCTION OF VACCINES AGAINST HUMAN HEPATITIS VIRUSES B (HBV) AND C (HCV) 2014-2017 Research Council of Norway Nano 2021 program SaNoVac: Nanoparticle encapsulation of a plant-produced vaccine against piscine reovirus infection in salmon 2009-2014 Project leader for “Expression and evaluation of envelope domain III-based experimental tetravalent dengue vaccine using a tobacco chloroplast expression system” a Research Council of Norway: Global Health and Vaccine Research (GLOBVAC) program funded project. 2013-2016 Leader of Workpackage (WP2) 2 on a Strategic Institute program: Choices and opportunities for sustainable use of phosphorus in food production. 2012-2016 Responsible for WP4 on a Strategic Institute program on bioactive compounds in terrestrial of Arctic ecosystem. 2013-2016 Participant to a Strategic Institute program on SIS Radkulturer. 2010-2014 Project leader for “Cost effective, environmentally- and health-friendly production of poinsettia” a Research Council of Norway, BIONÆR program financed project. 2011-2013 Project partner for “Kryofrisk - Kryometoder for rensing av planter for plantepatogener og oppbevaring av friskt plantemateriale”, a Research Council of Norway and industrial partners jointly funded project. 2009-2011 Project leader for “Production of environmentally friendly transgenic poinsettia using SHI technology”, a project funded by University of Copenhagen, Denmark. 3 Curriculum Vitae Jihong Liu Clarke 2009-2011 2009-2011 2008-2013 2007- 2008 2006-2007 2005-2007 Co-project leader for “Attempt at producing vaccine against Atlantic halibut Noda virus (AHNV) in tobacco chloroplasts”. Collaborative project with University of Bergen/Marine Research Institute as the project owner. Project partner for “Maize genetic transformation”. Collaborative project with Sichuan Agricultural University, China who is the project owner/manager Representing Norway as a management committee member to EU COST Action FA0804 on Molecular Farming: plant production system for valuable proteins. Project leader for “New poinsettia with enhanced virus resistance” a Research Council of Norway “Verifiseringsprogrammet” funded project. Project leader for “BILAT: Norway-USA: Chloroplast genome sequencing of turfgrass Agrostis stolonifera” funded by the Research Council of Norway. Project leader for “Norway-China Biosafety of GM maize” a Research Council of Norway funded project. 12. Expertise and experiences I received my PhD in 2003 and have extensive experience in tissue culture and 2003genetic engineering of various plants including oilseed Brassica napus, barley, tobacco and poinsettia. Recently, I have established a research group focusing on genetic engineering of ornamental crops and molecular farming for plant produced human and fish vaccines. My research on transgenic poinsettia has also attracted interest in collaboration in the ornamental industry, both nationally (G3 Ungplanter) and internationally (PLA international (Denmark), Ornamental Bioscience in Germany, personal communication). In addition, I have acquired good experience of project management, international collaboration and networking. I have been the project leader for several projects funded by the Research Council of Norway shown above. Through these projects, I have established good international networks in China, North America and European countries. Since the year 2006, I have been coordinator for China relations at Bioforsk, to promote research cooperation between Bioforsk and Chinese research organizations. Up to date, Bioforsk has successfully developed collaborations with a number of research academies and universities such as the Chinese Academy of Agricultural Sciences in Beijing, Jilin Academy of Agricultural Sciences in Changchun, Liaoning Academy of Agricultural Sciences in Shenyang, and Heilongjiang Academy of Agricultural Sciences (HAAS) in Harbin. Recently, a Sino-Norwegian Low-carbon Agriculture Sustainable Development Research Center was opened on September 7, 2011 at HAAS, in Harbin, China). I was one of the key persons in making this Bioforsk-HAAS joint research center a reality. 13. Track records: For all my projects where I have a role either as the project leader or an active partner, I have 4 Curriculum Vitae Jihong Liu Clarke accomplished the assigned tasks and produced peer-reviewed publications as well as popular science articles (publication list in my CV). In addition, all the requested reports to NFR were sent in on time in all my projects. My genetically modified (GM) poinsettia research funded by NFR’s BIONÆR program was chosen as web news to be published on the homepage on December 21, 2012 (http://www.forskningsradet.no/no/Nyheter/Genteknologien_inntar_juleplantene/1253982688009 ?WT.mc_id=nyhetsbrev-ForskningsradetNorsk). The compact growth poinsettia plants we have generated have innovation potential. My research on low cost production of dengue vaccine in green plants has also made a big media hit with a 2-page report in the largest Norwegian national newspaper “Aftenposten” on March 5, 2013 (http://www.aftenposten.no/nyheter/iriks/Tobakkog-gronnsaker-blir-livsviktig-vaksine-7137697.html). This was the first time in more than 10 years that biological research from my institute has been reported in Aftenposten indicating the societal interest and importance of this “biotechnology for innovation” research that I have built up over many years with the full support of my national and international collaborators. 14. Reviewer experiences: Review manuscripts for journals during past three years (2010-2013) Review grant proposals for international foundations (2013) Plant Molecular Biology, PLoS One, BMC Evolutionary Biology, Plant Cell Rep, Journal of Experimental Botany, Molecular Biology Reports, Journal of Advanced Biotechnology, Molecular and Cell Protomics, Jorunal Plant Physiology, Gene, Acta Agriculturae Scandinavica, Section B - Plant Soil Science etc. National Science Foundation of China, The Swiss National Science Foundation 15: Selected peer-reviewed publications or editorials: 2014 Hassan SW, Waheed MT, Mueller M, Clarke JL, Shinwari ZK, and Lössl AG (2014) Expression of HPV-16 L1 capsomeres with glutathione-S-transferase as a fusion protein in tobacco plastids: an approach for a capsomere-based HPV vaccine. Human Vaccines & Immunotherapeutics 10: DOI10.4161/21645515.2014.970973 2014 2014 2014 Zhang ZB, Lee Y, Haugslien S, Sivertsen A, Skjeseth G, Clarke JL, Spetz C Wang QC Blystad D-R (2014). Cryotherapy alone could not eradicate Chrysanthemum stunt viroid from infected Argyranthemum maderense ‘Yellow Empire’. Acta Hort 1039: 201-208 M. Ashraful Islam, Danuˇse Tarkowská, Jihong Liu Clarke, Dag-Ragnar Blystad, Hans Ragnar Gislerød, Sissel Torre, Jorunn E. Olsen (2014) Impact of end-of-day red and far red light on plant morphologyand hormone physiology of poinsettia. Scientia Horticulturae 174 (2014) 77–86 Md Ashraf Islam, Tage Thorstensen and Jihong Liu Clarke (2014) Agrobacterium tumafasensmediated transformation of poinsettia (Euphorbia pulcherrima) In Kan Wang (ed.), Agrobacterium Protocols: Volume 2, Methods in Molecular Biology, vol. 1224, DOI 10.1007/978-1-4939-1658-0_27, © Springer Science+Business Media New York 2014 5 Curriculum Vitae Jihong Liu Clarke 2013 2013 2013 2013 2013 2012 2012 2011 2011 2011 2011 2010 2009 Jihong Liu Clarke, Mohammad Tahir Waheed, Andreas G Lössl, Inger Martinussen, Henry Daniell, (2013) How can plant genetic engineering contribute to cost-effective fish vaccine development for promoting sustainable aquaculture? Plant Mol Biol, 83(1) 33-40. DOI : 10.1007/s11103-013-0081-9 M Ashraful Islam, Henrik Lütken, Sissel Haugslien, Dag-Ragnar Blystad, Sissel Torre, Jakub Rolcik, Søren K Rasmussen, Jorunn E Olsen, Jihong Liu Clarke (2013) Overexpression of AtSHI gene in poinsettia (Euphorbia pulcherrima Willd. ex Klotzsch) resulting compact plants. PLoS ONE 8(1): e53377. doi:10.1371 Jihong Liu Clarke and Peng Zhang (2013) Plant biotechnology for food security and bioeconomy. Plant Mol Biol 83(1): 1-3. DOI: 10.1007/s11103-013-0097-1 Gottschamel, J., Waheed, M.T., Clarke, J.L., Lössl, A. (2013) A novel Chloroplast transformation vector compatible with the Gateway® recombination cloning technology. Transgenic Research DOI 10.1007/s11248-013-9726-3 Zhang ZB, Lee Y, Haugslien S, Sivertsen A, Skjeseth G, Clarke JL, Spetz C Wan QC Blystad D-R (2013). Cryotherapy alone could not eradicate Chrysanthemum stunt viroid from infected Argyranthemum maderense ‘Yellow Empire’. Acta Horticulturae (Accepted) Henrik Lütken, Jihong Liu Clarke, Renate Müller (2012) Genetic engineering and sustainable production of ornamentals: current status and future directions. Plant Cell Rep 31(7) 1141-1157, DOI 10.1007/s00299-012-1265-5 M Ashraful Islam, Goutam Kuwar, Jihong Liu Clarke, Dag-Ragnar Blystad, Hans Ragnar Gislerød, Jorunn E. Olsen, Sissel Torre (2012). A high blue light proportion provided by light emitting diodes (LEDs) results in compact poinsettia plants without change in production time. Scientia Horticulturae 147: 136–143 Clarke, J.L., Daniell, H. (2011) Plastid biotechnology for crop production: present status and future perspectives Plant Mol Biol 76 (3): 211-220 Spetz, C., Clarke, J.L., Dees, M.W., Haugslien, S., Moe, R. and Blystad, D.-R. (2011). RNA silencing-based resistance in Nicotiana benthamiana is transiently broken by graft inoculation of Poinsettia mosaic virus. Acta Hort 941:91-108 Clarke, J.L., Daniell, H., Nugent, J.M. (2011) Chloroplast biotechnology, genomics and evolution: Current status, challenges and future directions. Plant Mol Biol 76 (3) 207-209. Clarke, J.L., Spetz, C., Haugslien, S., Dees, M. W., Moe, R., Blystad, D.R. (2011) Production of Transgenic Poinsettia with Resistance against Poinsettia mosaic virus (PnMV) Using Agrobacterium-Mediated Transformation. Acta Horti 901: 87-94 Liu, N., Zhu, P., Peng, C., Kang, L., Gao, H., Clarke, N., Clarke, J.L. (2010) Effect on soil chemistry of genetically modified (GM) vs. non-GM maize. GM Crops 1(3): 1-5 Wu, R., Guo, W.L. Wang, X.R. Wang, X.L. Zhuang, T.T. Clarke, J.L., Liu, B. (2009) Unintended consequence of plant transformation: biolistic transformation caused transpositional activation of an endogenous retrotransposon Tos17 in rice ssp. japonica 6 Curriculum Vitae Jihong Liu Clarke 2008 2008 2007 2006 2006 1999 1996 1996 1995 1992 1989 1988 cv. Matsumae. Plant Cell Rep 28:1043–1051 Clarke, J.L., Spetz, C., Haugslien, S., Xing, S.C., Dees, M. W., Moe, R., Blystad, D.R. (2008) Agrobacterium tumefaciens-mediated transformation of poinsettia, Euphorbia pulcherrima, with hairpin RNA constructs confers resistance to Poinsettia mosaic virus. Plant Cell Rep 27:1027–1038 Xing, S.C., Clarke, J.L. (2008) Progress of chloroplast genome analysis. Progress in Biochemistry and Biophysics. 35(1) 1-8 Saski, C., Lee, S.B., Fjellheim, S., Guda, C., Jansen, R.K., Luo, H., Tomkins, J., Rognli, O.A., Daniell, H., Clarke, J.L. (2007) Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor and Agrostis stolonifera, and comparative analyses with other grass genomes. Theor Appl Genet 115(4): 571-590 Clarke, J.L., Klemsdal, S.S., Fløistad, E., Hvoslef-Eide, A.K., Haugslien, S., Moe, R., Blystad, D.R. (2006) Genetic engineering of poinsettia with the aim of enhancing its resistance to poinsettia mosaic virus. Acta Horticultura 722: 321-325 Klemsdal, S.S., Clarke, J.L., Hoell, I.A., Eijsink, V.G.H., Brurberg, M.B. (2006) Molecular characterization of a novel endochitinase gene (ech30) from the mycoparasitic fungus Trichoderma atroviride strain P1. FEMS Microbiological letters. 256: 282-289 Liu Clarke, J.-H., Chèvre, A.-M., Landgren, M., Glimelius, K. (1999) Characterization of sexual progenies of male-sterile somatic cybrids between Brassica napus and Brassica tournefortii. Theor Appl Genet 99: 605–610 Li, W.-J., Liu, J.-H., Li, Y.-P., Liu, F. (1996) Production and cytogenetic analysis of intergeneric hybrids between Elymus anthosachnoides and Psathyrostachys huashanica (Triticeae, Poaceae). Plant Systematics and Evolution 202: 265-269 Liu, J.-H., Landgren, M., Glimelius, K. (1996) Transfer of B. tournefortii cytoplasm to B. napus for production of cytoplasmic male sterile (CMS) B. napus. Physiologia Plantarum 96: 123-129 Liu, J.-H., Dixelius, C., Glimelius, K. (1995): Brassica napus (+) B. tournefortii, a somatic hybrids containing traits of agronomic importance for rapeseed breeding. Plant Sci. 109: 75-86. Lu, B.-R., Liu, J.-H., (1992): Genome analysis and biosystematics of the wheat tribe (Triticeae Dumort). Chinese Bul. Botany, 9 (1): 26-31 Liu, J.-H. (1989): A dialle analysis of peroxidase isozyme on maize Chinese J. Genetics. 15(2): 157- 161 Liu, J.-H. (1988): Diallel analysis of peroxidase isoenzymes in maize (in Chinese). Acta Genetica Sinica, 15(4): 265- 269 15. Selected popular scientific publications and other publications 1. Jihong Liu Clarke and Bjørn Kåre Myskja (2014) Tobakksplanten som medisinfabrikk. Debatt, Aftenposten November 6, 2014, side 12. 7 Curriculum Vitae Jihong Liu Clarke 2. Andreas G Lössl & Jihong Liu Clarke (2013) Molecular pharming: manufacturing medicines in plants. Immunotherapy (2013) 5(1), 9–12 3. Jihong Liu Clarke and Peng Zhang (2013) Plant biotechnology for food security and bioeconomy. Plant Molecular Biology. DOI: 10.1007/s11103-013-0097-1 4. Camilla Østerud, M Ashraful Islam, Jorunn E Olsen, Dag-Ragnar Blystad, Sissel Torre, Trine Hvoslef-Eide, Jihong Liu Clarke (2013) Genetic engineering of GA biosynthesis by overexpressing PcGA2ox1 for restricting poinsettia plant height. Bioforsk FOKUS 8(2) 386 5. Md. Ashraful Islam, Henrik Lütken, Sissel Haugslien, Sisel Torre1, Jorunn E Olsen, Dag Ragnar Blystad, Søren K. Rasmussen, Jihong Liu Clarke (2013) Overexpression of Arabidopsis SHORT INTERNODE (Atshi) gene in poinsettia ,Euphorbia pulcherrima, results in compact plants. Bioforsk FOKUS 8(2) 385 6. Jihong Liu Clarke (2012) Broen mellom biomaterialer og økonomi. NFR Bladet Forskning juni nr 2, 2012 p 24. 7. Jihong Liu Clarke (2012) Planter er grønne fabrikker. Nationen 4. February 2012, p 26. 8. Jihong Liu Clarke and Carl Spetz (2012) Potato Biotechnology - a useful way to promote food security and bioeconomy. Bioforsk Fokus 5(2) 106-107. 9. M. Ashraful Islam, S. Haugslien, D.R. Blystad, S. Torre, J.E. Olsen, H. Lütken, J. Liu Clarke (2012) Agrobacterium mediated transformation of AtSHI gene in poinsettia (Euphorbia pulcherrima), resulting in compact plants. Bioforsk Fokus 5(2) 191-192 10. E.S. Riiser, I. Holtsmark, H.S. Steen, S. Swaminathan, N. Khanna, R. Bock, J.L. Clarke (2012) Low cost production of tetravalent dengue vaccine in tobacco chloroplasts. Bioforsk Fokus 5(2) 193-194 11. Blystad, D.R., Clarke, J.L. (2011) Kryoterapi for rensing av plantemateriale. Bioforsk Fokus. 6(2): 32-33 12. Clarke, J.L., Blystad, D.R., Fløistad, E. (2010) Julestjerne – populær blomst og nyttig forskningsverktøy. Hagetiden 10: 64, Oktober 2010. 13. Clarke, J.L., Riiser, E.S., Guo, S, Klemsdal, S.S. (2010) Tobakkplanter som fabrikk for produksjon av vaksiner. Bioforsk Fokus 5(2): 74-75. 14. Clarke, J.L., Spetz, C., Haugslien, S., Fløistad, E., Blystad, D.R. (2010) 15. Virusresistente julestjerner ved genetisk transformasjon, Bioforsk Fokus.5 (2): 66-67. 16. Clarke, J.L. (2009) Bioteknologi og matsikkerhet. Nationen, November 25, 2009. 17. Clarke, J.L., Klemsdal, S.S., Takle, G.W. (2006) Genmodifisering av kloroplaster -en bedre vei til sikre GMO? GenIalt 4: 12-13 18. Clarke, J.L., Spetz, C., Haugslien, S., Fløistad, E., Blystad, D.R. (2005) Flytting av virusgener – resistens hos julestjerne? Gartneryrket nr 10 (2005): 29. 19. Clarke, J.L., Tronsmo, A., Clarke, N., Klemsdal, S.S. (2004) Quantification of the expression of chitinolytic enzyme encoding genes ech30, ech42 and nag1 in Trichoderma atroviride stain P1 under varying growth conditions using real-time RT-PCR assay. Journal of Zhejiang University 30(4): 428. 8 Curriculum Vitae Jihong Liu Clarke 20. Clarke, J.L. (2003) Molecular study of chitinolytic enzyme encoding genes in Trichoderma atroviride strain P1 using real-time RT-PCR and the utilization of the genes in transgenic barley. PhD Thesis 2003:16. 21. Clarke, J.L., Brekkå, N., Klemsdal, S.S. (2001) The use of chitinase genes from Trichoderma atroviride (T. harzianum) P1 to produce transgenic plants with possibly improved resistance. Proceedings of 10th MPMI, p. 240. 22. Liu Clarke, J.-H. (1997) Cytogenetic analysis of sexual progenies of male sterile somatic cybrid between B. napus and B. tournefortii. In: Tamás Lelley (ed) Current Topics in Plant Cytogenetics Related to Plant Improvement. WUV-Universitätsverlag, Austria, pp. 140-147. 23. Liu, J.-H. (1995) Production and analyses of somatic hybrids and cybrids between Brassica napus and B. tournefortii. Licentiate Thesis. ISBN 91-576-5004-7, Uppsala 1995. 24. Liu, J.-H., Gao, Z.-R. (1987): Analysis and relation of the combining ability of peroxidase isozymes and yield on maize. J. Sichuan Agric. Univ., 5(3): 209-214. 16. Media coverage 1. www.nrk.no/dagsrevyen 8. oktober, 2014 2. www.tv2.no 8. oktober,2014 3. www.bioforsk.no 8. okt 2014 4. www.bioforsk.no 6. nov 2014 5. http://www.aftenposten.no/nyheter/iriks/Tobakk-og-gronnsaker-blir-livsviktig-vaksine7137697.html 6. (http://www.forskningsradet.no/no/Nyheter/Genteknologien_inntar_juleplantene/1253982 688009?WT.mc_id=nyhetsbrev-ForskningsradetNorsk) 7. Bioforsk nyhet 08.02.2011: “Kuldesjokk gir friskere planter” av Jon Schärer 8. NFR kveldsnytt på tobacco chloroplast produced vaccine project. (http://www.nrk.no/nett-tv/indeks/233713/) 9. Forskning.no 14.04.2010 “Kan tobakksplanten redde millioner av menneskeliv?” av Morten Günther (www.forskning.no) 10. Moss Avis lørdag 10. 02.2007 side 11: “Forsker tok prisen.” Av Jon Gran 11. Moss Dagblad 29.08.2003 side 2: “Med Doktorgrad i biologi” av Lars Rune Debes 12. Akershus Amtstidende 7. mai 2003 side 3: Doktorgrad 13. Moss Dagblad 7. mai 2003 side 7: “Forsvarer doktograd” av ØW 14. Vestby Nytt Nr 4, 2010 “Tobakksplanten kan redde liv” av Arne Eriksen. 17. Main invited talks, oral presentations and posters (only 2013) 1. Jihong Liu Clarke (2014) Expression of envelope domain III (EDIII) based dengue antigens in tobacco chloroplasts and their molecular analyses. 50th NBS kontaktmøte 9 Curriculum Vitae Jihong Liu Clarke January 23-26, Røros, Norway 2. Jihong Liu Clarke (2014) Green plants for bioenergy and aquaculture fish health. Bioforsk konferansen 2014. February 5-6, Hamar, Norway 3. Jihong Liu Clarke (2014) Chloroplast engineering for the production of high value recombinant proteins. November 3-4, Oslo Gardermoen, Norway 4. Jihong Liu Clarke, Johanna Gottschamel, Hege Steen, Sissel Haugslien, Andreas Lössl, Stephanie Ruf, Ralph Bock (2013) Molecular and immunological analyses of envelope domain III (EDIII) based dengue antigens expressed in tobacco chloroplasts. The 8th Global Health and Vaccination Research Conference, October 16-17, 2013 Bergen. Poster. 5. Jihong Liu Clarke (2013) Kan tobakksplanter redde liv? NatureViterForum 2013, November 13-14, 2013, Trondheim, Norway. Invited talk. 6. Jihong Liu Clarke (2013) How can plant genetic engineering contribute to costeffective fish vaccine development for promoting sustainable aquaculture? PlantBio 2013, November 7-8, 2013, Tromsø. Norway. Invited talk 7. Johanna Gottschamel, Waheed M Tahir, Andreas Lossl and Jihong Liu Clarke (2013) Expression of envelope domain III (EDIII) based dengue antigens in tobacco chloroplasts and their molecular analyses. PlantBio 2013 November 7-8, 2013, Tromsø, Norway. Oral presentation. 8. Zhang ZB, Clarke JL,Haugslien S, Sivertsen A, Skjeseth G, Lee Y, Spetz C, Rønningen JH, Søgnen HO, Ende P van der , Bjelland B, Wang QC, Blystad D-R (2013) Cryopreservation: an ideal technology for long-term plant genetic resources conservation and plant pathogen eradication. PlantBio 2013 November 7-8, 2013, Tromsø, Norway. Oral presentation. 9. Yanliang Wang, Sissel Haugslien, Marit Almvik, Nicholas Clarke, Anne Falk Øgaard, Jihong Liu Clarke (2013) Molecular and biochemical analysis of plant root exudate response to phosphorus starvation. PlantBio 2013 Nov 7-8, 2013, Tromsø, Norway. Poster 10. Zhang ZB, Lee Y, Haugslien S, Sivertsen A, Skjeseth G, Clarke JL, Spetz C Wang QC,Blystad D-R (2013). Elimination of Chrysanthemum stunt viroid (CSVd) from infected Argyranthemum maderense ‘Yellow Empire’, Abstract on International Workshop on Viroids and Satellite RNA (IWVdS), Beijing, China. Poster 11. Jihong Liu Clarke, Johanna Gottschamel, Hege Steen, Sissel Haugslien, Hanne Skomedal, Andreas Lössl, Stephanie Ruf, Ralph Bock (2013) Expression of envelope domain III (EDIII) based dengue antigens in tobacco chloroplasts and their molecular analyses. Plant Based vaccine and antibodies (PBVA 2013), 5-7 June 2013, University of Verona, Italy. Poster 12. Jihong Liu Clarke (2013) Expression of envelope domain III (EDIII) based dengue antigens in tobacco chloroplasts and their molecular analyses. The 3rd International Symposium on Chloroplast Genomics and Genetic Engineering (3rd ISCGGE), May 8 - May 11, 2013, New Brunswick, NJ USA. Invited talk 10 Curriculum Vitae Jihong Liu Clarke 13. Jihong Liu Clarke (2013) Mikroalger- en fremtidsrettet biofabrikk for produksjon av fiskevaksiner. Årsmøte Norsk Algeforening, med fagseminar "Alger til nytte" May 23, 2013, Bergen. Invited talk 14. Johanna Gottschamel, Waheed M Tahir, Jihong Liu Clarke and Andreas Lossl (2013) Convenient construction of chloroplast transformation vectors by a recombinant cloning technology. The 3rd International Symposium on Chloroplast Genomics and Genetic Engineering (3rd ISCGGE), May 8 - May 11, 2013, New Brunswick, NJ USA. Poster 15. Waheed M Tahir, Johanna Gottschamel, Jihong Liu Clarke and Andreas Lossl (2013) Overcoming chlorosis and growth retardation in tobacco transplastomic LTB-L1 plants via inducible expression. May 8-11, 2013, New Brunswick, USA. Poster 16. Martin Møller Hansen, Uffe Bjerre Lauridsen, Josefine Nymark Hegelund, Renate Müller, Jihong Liu Clarke, Henrik Lütken (2013) Agrobacterium rhizogenes mediated transformation of Rhodiola sp. – an approach to enhance the level of bioactive compounds. International Symposium on Medicinal Plants and Natural Products. Montreal Botanic Garden; June 17-19, 2013. Oral presentation 17. Jihong Liu Clarke (2013) Overexpression of the Atshi Gene in Poinsettia (Euphorbia pulcherrima) Results in Compact Plants. The 2013 ASHS Annual Conference, July 21-27, 2013. California, USA. Oral presentation 18. M Ashraful Islam, Henrik Lütken, Sissel Haugslien, Hege S Steen, Dag-Ragnar Blystad, Sissel Torre, Jakub Rolcik, Søren K Rasmussen, Jorunn E Olsen, Jihong Liu Clarke (2013) Overexpression of the Atshi Gene in Poinsettia Results in Compact Plants. 7th EPSO Conference: Plants for a Greening Economy. Greece, September 1-4, 2013. Poster 11