APSU IACUC
Minutes from 2-6-07
Members Present:
Members Absent:
Sarah Lundin-Schiller, Chair
Gilbert Pitts, Biology
Philenese Slaughter, Library (non-scientist)
Paul Nicodemus, Psychology
Chiu-Fu Sheng, Student Representative
Jack Caldwell, Agriculture
Charles Pinder, Dean Graduate Studies (Ex officio)
Jenifer Kennedy, Director Grants & Sponsored Programs (Ex officio)
Ben Howard, D.V.M. (unaffiliated veterinarian)
Amanda Fant, D.V.M. (affiliated veterinarian)
Meeting called to order: 2:00 PM
Quorum Status: 5 voting members required; 6 present.
Old Business:
1) EEC Inspection Report was signed by members present and this was sent to be placed on file
in Grants Office.
2) Minutes of 1-16-07 Approved; 6 in favor and 0 opposed; Also on file in Grants Office.
New Business:
1) Protocol 07-005, Dr. Chad Brooks; Committee voted 6-0 to return for revision. The
following items need to be clarified or revised:
 check the room number of the SSC animal facility; correct if necessary.
 Section IIB. Detail the volume of each injection given to the mice.
 Section IIB. Clarify numbers of animals; it is suggested that you state you will use 10
infected; 10 non-infected and that all organs will be analyzed from all animals.
 Section II B. Item 1, second sentence – correct “were” to “will be”.
 Section IIB. Add to the procedures the information which is given later in the form about
taking ear punch biopsy twice per week.
 Section III B. Clarify sentence about LPS or omit. Give explanation of why the proposed
species is most appropriate.
 Section IIIC. Provide a reference that ten mice is the statistical standard for all vaccine
challenge experiments.
 Section IVD. Clarify handling procedure or other procedures that will help to reduce
discomfort, distress, pain, and injury. Omit “None needed”.
 Section VB. Describe the carcass disposal procedure.
 Section VI. List the actual modules of the Research Training that you and your student
have completed.
2) Report on Occupational Health and Safety in the Care & Use of Animals.
Subcommittee is making progress in assessing current needs based on animals being used here at
APSU and gathering resources many of which can be posted on the IACUC website for animal
users to access.
Dr. Caldwell reports that Chad Pugh spoke with Agricultural Extension Officer and found that
they did not have any Occupational Health and Safety training videos or other materials currently
available for farm workers. Dr. Caldwell was going to continue checking with the Extension
office in Nashville.
3) Report on Role of IACUC in overseeing research involving wildlife
Dr. Pitts provided a written report attached below reporting on the use of wildlife in research and
the role that the IACUC plays in overseeing such research. It is clear that at most institutions
researchers using wild animals are required to complete a protocol and receive IACUC approval
prior to research. We will need to develop or adopt an appropriate form for researchers here at
APSU. The information gathered by Dr. Pitts and others on the committee will also be made
available to all researchers through the website.
Meeting adjourned at 3:00 PM
Use of Wildlife in Research and IACUC Role in Overseeing Report Attached Below
REPORT ON THE USE OF WILDLIFE IN RESEARCH
Presented by Gilbert Pitts to the APSU IACUC committee on 6FEB2007
I found statements at the University of Minnesota stating the responsibility of IACUC in
overseeing animal research.
I found excellent information regarding the oversight of wildlife studies at the University of
Kansas, and the University of Kentucky.
I sent e-mails to OLAW and the University of Kentucky requesting information concerning the
care and use of wild animals. I am still awaiting a response from OLAW. Dr. Larry Iten from
the University of Kentucky has already responded. He will send me a copy of an approved field
research protocol so that we can see their procedure.
University of Minnesota
2. What is subject to review
2.1 Summary
The IACUC has a broad responsibility. Generally, the committee reviews any use of live animals in research,
teaching, service or display by faculty, staff, or students affiliated with the University of Minnesota,
regardless of where the activity occurs and of the activity's funding source.
In short, all animals used by University personnel for University purposes or owned by the University of
Minnesota come within the scope of the IACUC's review. These animals are involved in a variety of uses and
in a range of disciplines.
2.2 Research
Many of the animals covered in IACUC review are used in research, including agricultural research (such as
the study of food and fiber production or diet manipulation) as well as medical, biological, and behavioral
research. Most of these animals are acquired and housed by the University, but some are free-ranging
wildlife, or client-owned animals.
2.3 Teaching
Animals used in educational settings are also protected by IACUC review. Examples include those used to
teach agricultural techniques, animal husbandry, and medical and veterinary procedures.
2.4 Service and Display
Animals that perform tasks or appear in exhibits or demonstrations are also covered. Examples include the
horses used by the St. Paul mounted police, the animals kept at the Raptor Center, and fish in tanks in
University buildings.
University of Kentucky
http://www.research.uky.edu/ORI/univet/resources/Handbook/hb-policies-field-research.htm
Animal Resources and Procedures Handbook
University of Kentucky
POLICIES/PROCEDURES PERTAINING TO:
FIELD RESEARCH, AND CARE AND USE OF LOWER ANIMALS
All vertebrate animals used in teaching or research at the University of Kentucky must be used according to a
protocol approved by the Institutional Animal Care and Use Committee.
Field Research
The IACUC has developed the following policy regarding field research:
It is the policy of the University of Kentucky that every research project involving animals, including field
research, must be conducted under a protocol approved by the Institutional Animal Care and Use Committee.
This policy does not apply to research or teaching which involves observation of animals in their natural
habitat when they are not captured or subjected to unnatural environment or treatments. Appropriate permits or
licenses and close adherence to applicable federal and state laws and regulations must be practiced. Kentucky
statutes governing wildlife regulations are available from the Office of the University Veterinarian.
Various professional societies have developed standards for the conduct of field research. Following are the
names of four sets of guidelines now available from the Office of the University Veterinarian on request. They
are excellent references and the appropriate one(s) should be read by an investigator prior to using animals.
l. ACCEPTABLE FIELD METHODS IN MAMMALOGISTS. Prepared by the American Society of
Mammalogists.
2. GUIDELINES FOR USE OF FISHES IN FIELD RESEARCH (1987). Prepared by the American Society of
Ichthyologists and Herpetologists, the American Fisheries, the American Institute of Fisheries Research
Biologists.
3. GUIDELINES FOR USE OF LIVE AMPHIBIANS AND REPTILES IN FIELD RESEARCH (1987).
Prepared by the American Society of Ichthyologists and Herpetologists, the Herpetologists' League, the
Society for the Study of Amphibians and Reptiles.
4. GUIDELINES TO USE OF WILD BIRDS IN RESEARCH (1997). Prepared by the American
Ornithologists' Union, the Cooper Ornithological Society, Wilson Ornithological Society.
University of Kansas
http://www2.ku.edu/~acu/fld_study.shtml
Note: The powerpoint presentation is attached at the end of this document.
Field Biology IACUC Requirements
At its December 2004 meeting, the KU Institutional Animal Care and Use Committee (IACUC) approved revised
procedures for investigators (faculty, staff, students) whose field research involves live vertebrates. The resources on
this page will assist investigators in preparing a Statement of Field Research on Live Vertebrates and related forms
required by the University and funding agencies.
Investigators are encouraged to begin by viewing our PowerPoint presentation The University of Kansas IACUC and
Field Research.
Netscape Users: If upon clicking the presentation you get a browser window
containing what seems a jumbled mass of letters and other text characters, it
means you do not have your browser configured to use PowerPoint or PowerPoint
Viewer for presentations. Reconfigure your browser. With newer operating
systems, you may be able to click the link and hold the right mouse button down
(Macintosh users- just hold the one button down), getting a menu of options, of
which one will be to save the file to your drive. In that case, do so and then open it
with either of the two programs mentioned. This issue does not affect users of
Explorer, which comes preconfigured to correctly render PowerPoint presentations.
As you move through the presentation, you should take time to download and examine links to professional society
guidelines applicable to your study. The last slide of the presentation contains links to the forms you are required to
complete and submit to the KU IACUC prior to commencement of your study. For convenience, these forms also are
linked to this page, below, for future reference.
Checklist of IACUC Requirements for Field Research
Statement of Field Research on Live Vertebrates
Health Assessment Form
Periodic Health Review Form
Requirements for Field Research Approval
I.
II.
Institutional Animal Care and Use Committee (IACUC) Approval – All live vertebrate
field research performed under the auspices of the University of Kansas, Lawrence campus
must receive approval from the IACUC prior to initiating animal work. To secure
approval, a Statement of Field Research form must be completed. In all instances,
Principal Investigators are responsible for certifying familiarity with guidelines specific to
their field of study (see below).
A.
Full Field Research. In the case of hands-on field research performed by KU
scientists, the entire Statement of Field Research form must be completed.
Orientation and a Health Assessment Form must also be completed.
B.
KU Funding of Another Institution’s Field Research. If the University of Kansas,
Lawrence campus, funds another institution’s field research, answer questions #1-5,
question #7, and sign the Statement of Field Research form. Submit this document
with a copy of the other institution’s completed and approved field statement. This
must include the corresponding approval letter signed by the chairperson or other
authorized signatory of the other institution’s IACUC.
C.
Observation Only. When field research involves SOLELY observation of
undisturbed animals, an accelerated review process can be initiated. Complete
Questions #1-4, Question #6, and Question #12 on the Statement of Field Research.
Sign and submit to the IACUC. A Health Assessment Form must be completed.
Mentors must provide proper instruction and oversight of all students and others
assisting in Observation Only studies.
Specific Requirements
A.
Attend Field Research Orientation – An orientation tailored specifically for field
research will be coordinated on an as-needed basis for those participants who plan to
work with vertebrates in the field. When an orientation session needs to be
scheduled, the Principal Investigator will send a written request to the IACUC at least
two weeks in advance of anticipated need-date. To be included in the request are
days and times when participants can be available for a 1.5-hour block of time to
attend. Orientation is held in the Animal Care Unit conference room. This
orientation will be unique to your project, and will involve such things as Animal
Care Unit facilities you may need (if any), special health considerations including
allergies, hantavirus, histoplasmosis, etc.
B.
Completion of a Health Assessment form – These forms will be provided to
participants at the beginning of each orientation session. Alternatively, the form can
be found at the Animal Care Unit website (www.ku.edu/~acu/acu.html) “Forms” link
(Health Assessment Form). Completed forms should be sent to Watkins Health
Center. Due to confidentiality issues, Watkins Health Center cannot accept
electronic submission of medical history forms. Please do not send completed forms
to Watkins electronically.
NOTE: The University’s occupational health program requires that participant
health status be evaluated on an annual basis. In each subsequent year of
involvement in field research, participants are required to complete a Periodic Health
Review form. The IACUC Coordinator will contact the Principal Investigator
annually to determine the active status of participants listed on their Statements of
Field Research and coordinate completion of Periodic Health Review forms as
needed.
C.
Review Class-Specific Guidelines – It is the Principal Investigator’s responsibility
to be familiar with the guidelines applicable to a given project and for these to be
reflected clearly in the Statement of Field Research form submitted to the IACUC.
By signing the Statement of Field Research form, you certify that you, and other
persons actively involved in this research, have read and are familiar with all
applicable guidelines. Guidelines can be found at the following web addresses.
Guidelines for Proper Care and Use of Wildlife in Field Research. The Wildlife Society:
National Wildlife Federation, 1412 16th Street N.W., Washington, D.C. 20036. The
Wildlife Biologic Society: 7101 Wisconsin Ave. #611, Washington D.C. 22014.
http://www.nwhc.usgs.gov/pub_metadata/field_manual/chapter_6.pdf
Guidelines to the Use of Wild Birds in Research. The Ornithological Council: Providing
Scientific Information about Birds. Special Publication 1997. Second Edition 1999.
Edited by Abbot S. Gaunt & Lewis W. Oring
http://www.nmnh.si.edu/BIRDNET/GuideToUse/tableofcontents.htm
Guidelines for the Use of Fishes in Research, American Society of Ichthyologists and
Herpetologists, American Fisheries Society, American Institute of Fishery Research
Biologists. American Fisheries Society, 5410 Grosvenor Lane, Bethesda, MD 20814.
Copyright 2004.
http://www.fisheries.org/html/Public_Affairs/Sound_Science/Guidelines2004.shtml
Guidelines for the Capture, Handling, and Care of Mammals, as approved by the American
Society of Mammalogists, prepared by the Animal Care and Use Committee.
http://www.mammalogy.org/committees/commanimalcareuse/98acucguidelines.PDF
Guidelines for Use of Live Amphibians and Reptiles in Field and Laboratory Research.
Revised by the Herpetological Animal Care and Use Committee (HACC) of the
American Society of Ichthyologists and Herpetologists, 2004.
http://www.asih.org/pubs/ASIH_HACC_Final.PDF
IACUC Proposal Number:
Statement of Field Research on Live Vertebrates
University of Kansas, Lawrence
1.
Principal Investigator:
2.
Project Title or Course Name and Number:
3.
Contact Information (Complete grid below):
Office Phone
Department
4.
Emergency Contact Information
(Name / Phone Number)
Type of Project: Select the type of project by placing an ‘X’ in the box to the left of
the appropriate row of the table provided below. For those projects listed as
‘other,’ please state the purpose of the field research.
Research involving more than just observation
Observation ONLY – If research involves SOLELY observation of undisturbed
animals, complete Questions #6 and #12, sign/date and submit to the IACUC
via the Animal Care Unit.
Teaching
Other:
5.
Category: Select the category listed below by placing an ‘X’ in the appropriate
row of the table provided below.
New Proposal
Periodic Review*
Modification to
Secure Approval
Resubmission to
New Granting
Agency
Revision
All field research is reviewed on a 2.5 year basis.
New and/or revised text should be indicated in bold text.
Question #7 must be completed and a signature of compliance
provided.
Revised information must be indicated in bold text in the
narrative of this Animal Use Statement.
KU Funding of
another
Institution’s Field
Research
Answer questions #1-5, question #7, and provide a signature.
Submit this document with a copy of the other institute’s
completed and approved field statement. This must include
the corresponding approval letter signed by the chairperson or
other authorized signatory of the other institution’s IACUC.
*If this is a periodic review, please provide the following information:
Total Number
Number of animals used/trapped since last IACUC review:
Number of animals collected (brought to KU) since last IACUC
review:
6.
Species Used: The following grid must be completed. Place an ‘X’ in the box to
the left of the row which best describes the type of research. Descriptions of
procedure classes are available at the Animal Care Unit website:
www.ku.edu/~acu/acu.html.
Class of
Research
# of Animals Used
and/or Collected*
Type of Study
Broad scale faunal study
Study targets several particular species or higher taxa
(provide additional information below)
* Number provided must correspond with justification of numbers provided in the
response to Question #9b. In some instances, such as broad-scale faunal studies or
seine hauls, numbers may be estimated.
If your study targets several particular species or higher taxa, please list here:
7.
Funding Sources:
Title of Grant Proposal or Source of
Funds (If personal or departmental funds
will be used, please state)
Date
Submitted
Agency
Submitted To
Account or Project #
(where applicable)
The National Science Foundation requires that each Institutional Animal Care and Use
Committee review proposals for field research. To facilitate uniform standards for this
work, NSF encouraged professional societies in the various vertebrate disciplines to
prepare class-specific guidelines. Allen Press, Inc distributed these with mailings of
professional journals beginning in December 1987. These guidelines can also be found
on the web. Principal Investigators (PI) can consult the website of the societies relevant
to their study for access to guidelines. It is the Principal Investigator’s responsibility to
be familiar with the guidelines applicable to a given project. By signing below, the PI
indicates that all of the above statements are true (and accurately reflect statements in
the application for funds to support this research), and that the PI and other persons
actively involved in this research have read, and are familiar with all applicable
guidelines.
Place an ‘X’ in the first column next to each guideline that has been reviewed as part of
this research:
Guidelines for Proper Care and Use of Wildlife in Field Research. The Wildlife
Society: National Wildlife Federation, 1412 16th Street N.W., Washington, D.C.
20036. The Wildlife Biologic Society: 7101 Wisconsin Ave. #611, Washington
D.C. 22014.
http://www.nwhc.usgs.gov/pub_metadata/field_manual/chapter_6.pdf
Guidelines to the Use of Wild Birds in Research. The Ornithological Council:
Providing Scientific Information about Birds. Special Publication 1997. Second
Edition 1999. Edited by Abbot S. Gaunt & Lewis W. Oring
http://www.nmnh.si.edu/BIRDNET/GuideToUse/tableofcontents.htm
Guidelines for the Use of Fishes in Research, American Society of Ichthyologists
and Herpetologists, American Fisheries Society, American Institute of Fishery
Research Biologists. American Fisheries Society, 5410 Grosvenor Lane,
Bethesda, MD 20814. Copyright 2004.
http://www.fisheries.org/html/Public_Affairs/Sound_Science/Guidelines2004.sht
ml
Guidelines for the Capture, Handling, and Care of Mammals, as approved by the
American Society of Mammalogists, prepared by the Animal Care and Use
Committee.
http://www.mammalogy.org/committees/commanimalcareuse/98acucguidelines
.PDF
Guidelines for Use of Live Amphibians and Reptiles in Field and Laboratory
Research. Revised by the Herpetological Animal Care and Use Committee
(HACC) of the American Society of Ichthyologists and Herpetologists, 2004.
http://www.asih.org/pubs/ASIH_HACC_Final.PDF
All appropriate permits for collecting and or operating at the site of this field study have
or will be obtained prior to the start of the study.
______________________________________
_____________________________
Signature of Principal Investigator
Date
Question #8: Scientific Objective – State the scientific objective of the study
Question #9: Rationale
Question #9a: State rationale for using live animals.
Question #9b: State your rationale for the numbers of animals used and/or collected.
The rationale must correspond with the total number of animals provided in the
response to Question #6. In some instances, such as broad-scale faunal studies or
seine hauls, numbers may be estimated.
Question #10: Narrative Description - Supply a complete narrative description of all
proposed use of animals in the study. This should include a description of any
techniques described in the Class of animal research pertinent to your study
Questions #11a through #11g: Describe each of the following as they apply to the
proposed study. IF AN ITEM IS NOT APPLICABLE, THIS SHOULD BE STATED AS
THE ANSWER TO THE QUESTION:
Question #11a: Live Trapping - Include type of trap used, length of time animals will
remain in the trap (provide range), what type of survival support is provided in the trap
(e.g.: type of bedding to be used, water, food, water change when collecting fish, etc.),
what provisions will be made to protect animals in live traps in the event of inclement
weather, how will animals be sheltered from direct sun, etc.
Question #11b: Captivity – If held in captivity longer than 12 hours, how long and
under what conditions.
Question #11c: Surgical Procedures - Provide a description of surgical procedures to
be performed, pre-operative preparation and post-surgical care. Aseptic technique must
be used for all survival surgeries. When applicable, provide a description of aseptic
technique used in the field. Complete Anesthetics, Supplemental Anesthetics, and
Analgesics grids below as well.
Anesthetics: If anesthetics are used, complete the grid below:
Procedure
Duration
Anesthetic
Requiring
of
Agent(s) to be
Species
Anesthetic
Anesthesia
Used
(Indicate Survival
or Non-Survival)
Route
Of Admin.
Dosage
(mg/kg)
Supplemental Anesthetics. Animals anesthetized for one hour or longer may require
supplemental anesthesia. Complete the grid below for each procedure requiring
supplemental anesthesia. List the following: species, what agent(s) will be used, route
of administration, and dosage.
*Supplemental Anesthetic
Route of
Dosage
Agent(s) to be Used
Administration
(mg/kg)
Species
* For each supplemental anesthetic listed, describe the method used to determine the need for
supplemental anesthesia
Method Used:
Analgesics: If analgesics are used following surgery, complete the grid below:
Analgesic Agent
Route of
Administration
Dosage
Frequency or
Duration of
Administration
Species
Question #11d: Administration of Compounds - Using a separate line on the grid
below for each compound and species, list the following: species, compound to be
administered, dose or dose range (e.g. mg/kg) and/or volume, site, route (p.o., i.p., i.m.,
s.c., etc.) and schedule to be used.
Species
Compound to be
Route Schedule
Dose / Range
Site
Administered*
Volume
* For each compound listed above, list potential toxic or immunological effects. If not
applicable, please state.
Answer:
Question #11e: Euthanasia Procedures - Provide method of euthanasia for each
species or other taxonomic level. If more than one euthanasia method will be used per
species, these should be listed separately in the grid below. Where applicable, include
drug dosage and route of administration. If a physical method of euthanasia will be
used, provide reference to the most recent applicable professional society guidelines
(e.g.: thoracic compression, gunshot, cervical dislocation, decapitation, etc.).
Route of
Method of
Drug Dosage
Administration
Decapitation
(Where applicable)
(Where applicable)
(Where applicable)
Species
Method Used
Question #11f: Controlled Substances or Hazardous Materials – If controlled
substances or hazardous materials are used, describe precautions taken for personnel
and animals.
Question #11g: Biopsy Techniques - Describe in detail any biopsy techniques such as
tissue collection, blood collection, etc. When collecting blood, provide the following
information: Site, quantity per bleed, frequency of bleeding, how long/total number of
bleeds. If collecting tissue, provide the following: age of animal tissue being collected
from, method used to collect tissue (i.e.: toe clip, ear punch, etc.), amount of tissue to
be collected.
Question #12: Training Requirements
Principal Investigators and participants must complete the following:
1. Field Research Orientation*
Orientation tailored specifically for field research will be coordinated on an as needed
basis for those participants who plan to work with vertebrates in the field. When an
orientation session needs to be scheduled, the Principal Investigator will need to send a
written request to the IACUC at least two weeks in advance of anticipated need-date
(iacuc@mail.ku.edu). To be included in the request are days and times when
participants can be available for a 1.5-hour block of time to attend. Orientation is held in
the Animal Care Unit conference room. This orientation will be unique to your project,
and will involve such things as Animal Care Unit facilities you may need (if any), special
health considerations including allergies, hantavirus, histoplasmosis, etc.
2. Annual Occupational Health Screening
Investigator and
Participant’s Name
Field Research
Orientation
Annual Occupational
Health Screening
* Participants involved SOLELY in observation of undisturbed animals, as indicated in
Question #4 of this document, do not need to complete the field research orientation
training requirement. An annual occupational health screening is required for this
group.
Posted: January 24, 2007
The Office of Laboratory Animal Welfare, NIH, the Scientists Center for Animal Welfare (SCAW), the University
of Tennessee Health Science Center, and the Animal Care Division, Animal and Plant Health Inspection
Service of the U.S. Department of Agriculture will present an IACUC-Advanced Workshop. The workshop will
be held March 9, 2007 at The Peabody Court Hotel in Memphis, TN.
IACUC-Advanced was developed by the Scientists Center for Animal Welfare to train individuals concerned
with science and appropriate animal welfare. This may include experienced IACUC members and
administrators, principal investigators, attending veterinarians, regulatory personnel and laboratory animal care
staff. The regional workshop format lets small groups discuss specific advanced topics that are relevant to
IACUC functions.
The conference agenda and a registration form are available on the SCAW website at
http://www.scaw.com/conference.htm .