MLAB 2434
Microbiology
Antimicrobial Susceptibility Testing
Learning Objectives
At the end of this unit, the student will be able to:
1. Determine the role of the Clinical and Laboratory Standards Institute
(CLSI) in the clinical microbiology laboratory.
2. List the methods for susceptibility testing.
3. Describe the inoculation, required medium, incubation, reading and
interpretation of each of the following:
a. Disk diffusion
b. E-Test
4.
Define the following:
a. Minimum inhibitory concentration (MIC).
b. Minimum bactericidal concentration (MBC).
5. List three factors to consider if antimicrobial sensitivity testing is
indicated.
6. Describe how drug formularies are chosen for health care institutions.
7. Differentiate between organisms that are susceptible or resistant to a
particular antimicrobial agent.
8. Describe how sensitivity testing is standardized.
9. Recognize types of automated sensitivity testing systems.
10. Cite advantages of automated sensitivity testing systems.
11. Describe the purpose and frequency of quality control in sensitivity testing.
12. Define the following:
a. MRSA
b. VRE
c. VRSA
d. ESBL
e. KPC
f. CRE
g. AmpC
7/22/2012