Attachment B.
Infectious Agents
General Project Title:
YES
N/A
Does your research involve potential human, animal or plant pathogens or infectious agents?
If “Not Applicable”, please skip Attachment B and proceed to the next applicable attachment.
If “YES” continue below.
i.
Infectious Agent Summary
Please check all that apply.
RG 1
RG 2
RG 3
RG 4 Infectious Agents will be used.
We will be introducing Microbial Agents in vivo.
We will be introducing microbial agents into animals or plants. (Complete Attachment G)
We will be introducing microbial agents into other microbes
We will be introducing microbial agents into cell cultures, organs, or tissues. (Complete Attachment C)
We will be deliberately transferring microbes into one or more human research participants.(Complete Attachment I)
We will be collecting environmental samples that contain microbial agents. (Also complete Attachment J)
Procedures will be performed with microbial agents that may be associated with an increased potential risk of
exposure to personnel. Please explain below.
We will be using cell lines (or modifications of cell lines) that may be perceived to significantly diverge from standard
practices in our field. Please explain below.
We will be performing (or directing others to perform) practices that may be perceived to significantly diverge from
standard practices in our field. Please explain below.
We will be handling or culturing more than or equal to 10 liters at any one time. (Complete Attachment H)
Provide additional information from the checked box above. Briefly summarize the overall goal and specific objectives of
the work with these materials. (e.g. inserting viral vectors encoded with antibiotic resistance into gram positive bacteria)
ii.
Microbial Agents
Microbe Source
(Genus, species,
strain) If using viral
vectors, include
answers for both
the original virus
and your
recombinant vector
Select
Agent*
RG
Replication
Competent
Route of
Transmission
Yes
Yes
Yes
Virulence
or
Toxicity
Susceptibility
and
Resistance
No
No
No
Microbial Agents in Cells/Animals If not applicable, check here
Microbe Source
Disease
or Toxins
Produced
(specify
species)
(humans,
animals,
plants)
Yes No
Yes No
Yes No
* If yes, please complete Attachment E.
iii.
Host
Range of
Agent
Procedures
Performed
Recipient of
rDNA
Yes
Yes
Yes
Yes
EHS, SDSU
Rev. 04/2014
Cells
Infected
Animal/Plant
Species
Used
Max
Conc.
.
Max
Volume
Method of
Delivery
Route of
Shedding/Excretion
& Interval
No
No
No
No
1/2
Attachment B of
General BUA Application
iv.
Exposure Prophylaxis
For each microorganism, consider the consequences of an accidental exposure (e.g., mucosal
splash, inhalation, or inoculation) which might occur during experimental handling. Consider that
organisms normally not pathogenic for healthy humans may become so when the natural barriers to
infection are circumvented. Prepare a response procedure. It could be a simple matter of washing
the wound with soap and water. If antibiotic treatment is recommended, please include information
about the antibiotic of choice and its availability. The exposure response plan should be posted in
the laboratory and a copy kept the in the laboratory Biosafety Binder.
Microbe
Symptoms
Incubation
Period
Period of
Communicability
Response Procedure
Prophylaxis
Submission
Submit completed Attachment B with General BUA Application to IBC Gateway
3519, MC 1933 and an electronic copy to ibc@mail.sdsu.edu.
EHS, SDSU
Rev. 04/2014
2/2
Attachment B of
General BUA Application