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FLORIDA ATLANTIC UNIVERSITY
INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE
Rev.5/09
FISH USE PROTOCOL FORM
WELCOME TO THE FAU PROTOCOL PROCESS FOR ANIMAL USE
1. PLEASE NOTE THE REVISION DATE. YOU SHOULD USE THE LATEST VERSION FROM THE WEB.
2. PLEASE COMPLETE ALL SECTIONS
A. SOME SECTIONS MAY NOT PERTAIN TO YOUR PARTICULAR PROTOCOL BUT IT IS STILL REQUIRED
INFORMATION. JUST INDICATE “NO” IF NOT APPLICABLE.
B. IF A MAJOR PARAGRAPH IS MARKED “NO,” THEN DO NOT FILL OUT THE SUBPARAGRAPHS.
C. IF A MAJOR PARAGRAPH IS MARKED “YES,” ALL SUBPARAGRAPHS MUST BE FILLED IN. IF NOT
APPLICABLE, THEN STATE, “N/A”
3. AFTER A DRAFT IS COMPLETED, THE PI MUST MAKE AN APPOINTMENT TO MEET WITH THE UNIVERSITY
VETERINARIAN. (FEDERAL REQUIREMENT)
4. ALL PERSONNEL ON THE PROTOCOL HAVE TO BE TRAINED BY THE UNIVERSITY VETERINARIAN BEFORE THE
PROTOCOL WILL RECEIVE FINAL APPROVAL.
5. ENROLLMENT IN THE MEDICAL MONITORING PROGRAM FOR ANIMAL USERS (OHP) IS ALSO REQUIRED BEFORE
FINAL APPROVAL.
6. THERE ARE AT LEAST 3 SIGNATURES THAT ARE REQUIRED BEFORE SUBMISSION – PI, UNIVERSITY
VETERINARIAN, AND DEPARTMENT CHAIRPERSON. (SECTIONS F & G)
7. SUBMIT THE PROTOCOL
A. AFTER ALL SECTIONS ARE COMPLETED
B. AFTER ALL SIGNATURES ARE OBTAINED
C. AFTER ALL REQUIRED PERMITS OR LICENSES ARE OBTAINED
D. SUBMIT
a. ONE COMPLETE ORIGINAL COPY WITH ALL SIGNATURES TO THE OFFICE OF RESEARCH INTEGRITY,
ROOM 328, BS-12, BOCA CAMPUS. INCLUDE PERMITS & ATTACHMENTS (INCLUDING GRANT, IF
APPLICABLE, SURGERY FORM, WILDLIFE FORM, ETC.)
b. ONE ELECTRONIC COPY OF PROTOCOL AND ALL ATTACHMENTS TO KRISTEN WARE
(KCWARE@FAU.EDU) OR ELISA GAUCHER (EGAUCHER@FAU.EDU)
8. FINALLY: PLEASE DELETE THIS PAGE BEFORE SUBMITTING. PUT YOUR CURSOR ON THE FRAME AND CLICK.
IT SHOULD BECOME A HASH LINE. THEN HIT THE DELETE KEY UNTIL THE TOP LINE IS DELETED. CLICK ONCE
MORE TO BRING THE PROTOCOL TO THE TOP OF THIS PAGE.
QUESTIONS? CALL THE UNIVERSITY VETERINARIAN, DR. SYLVIA GOGRAFE, AT 7- 4233 (SGOGRAFE@FAU.EDU)
OR IACUC OFFICE AT 7-0777.
FAU Fish Protocol Page 1 of 16
FLORIDA ATLANTIC UNIVERSITY
INSTITUTIONAL ANIMAL CARE AND USE COMMITTEE
Rev. 5/09
FISH USE PROTOCOL FORM
A. ANIMAL USE PROTOCOL SUMMARY
IACUC DATE STAMP: Shaded areas for IACUC use only.
PROTOCOL NUMBER:
APPROVAL DATES:
Original:
MODIFICATION DATE:
1st Renewal:
2nd Renewal:
A.1. PROTOCOL TITLE
A.2. PRINCIPAL INVESTIGATOR (PI) NAME AND ADDRESS
First Name
Department
Last Name
Work Phone
Email Address
After Hours Phone
A.3. PROJECT PERSONNEL
(Include names of all individuals involved with the animals and this project) .
ROLE IN PROJECT (please answer all that apply)
Name
Work Phone
Email Address
For additional names copy section and add at the end of a section.)
Animal Contact:
 NONE (Not required to answer species and jobs below; General Training req’d)
 YES (Have to answer all below)
TRAINING: Enter dates for FAU hands on or online training (If doing Anest. and Surg., it
requires Surg. Training; Euthan. requires Euthan. Training). Also, mark an X under “OHP Enroll” to
indicate if you are enrolled in the FAU Medical Monitoring Program for Animal Users.
Species
Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Species
Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Discuss previous training and
background working with each
species and each procedure
marked including when trained
and how trained.
Name
Work Phone
Email Address
Animal Contact:
 NONE (Not required to answer species and jobs below; General Training req’d)
 YES (Have to answer all below)
TRAINING: Enter dates for FAU hands on or online training (If doing Anest. and Surg., it
requires Surg. Training; Euthan. requires Euthan. Training). Also, mark an X under “OHP Enroll” to
indicate if you are enrolled in the FAU Medical Monitoring Program for Animal Users.
Species Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Species
FAU Fish Protocol Page 2 of 16
Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Discuss previous training and
background working with each
species and each procedure
marked including when trained
and how trained.
Animal Contact:
 NONE (Not required to answer species and jobs below; General Training req’d)
Name
Work Phone
Email Address
 YES (Have to answer all below)
TRAINING: Enter dates for FAU hands on or online training (If doing Anest. and Surg., it
requires Surg. Training; Euthan. requires Euthan. Training). Also, mark an X under “OHP Enroll” to
indicate if you are enrolled in the FAU Medical Monitoring Program for Animal Users.
Species Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Species
Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Discuss previous training and
background working with each
species and each procedure
marked including when trained
and how trained.
Animal Contact:
 NONE (Not required to answer species and jobs below; General Training req’d)
Name
Work Phone
Email Address
 YES (Have to answer all below)
TRAINING: Enter dates for FAU hands on or online training (If doing Anest. and Surg., it
requires Surg. Training; Euthan. requires Euthan. Training). Also, mark an X under “OHP Enroll” to
indicate if you are enrolled in the FAU Medical Monitoring Program for Animal Users.
Species Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Species
Handling/monitoring Non-Surg Procedure General
Anest/Surgery
Euthanasia
OHP Enroll
Discuss previous training and
background working with each
species and each procedure
marked including when trained
and how trained.
To add more people, place your pointer at the top, left corner of the above group (just outside of the “N”), right click, copy and then paste just
below this line. Add as many as required.
EMERGENCY CONTACT
A.4. Daily and weekend contact numbers for emergencies. (Please provide PI and an alternate’s emergency info.)
Personnel listed in this section must also be listed as project personnel in the above section
PI
Alternate Person
B.1. TYPE OF SUBMISSION (CHECK ONE)
New Protocol
Year 3 Renewal of IACUC Protocol #
Identical to IACUC Protocol #
B.1.a. FUNDING SOURCE(S)
B.2. PROJECT START DATE
FAU Fish Protocol Page 3 of 16
Emergency Phone
Emergency Phone
PROVIDE RELATED IACUC NUMBERS
(Not applicable for new protocols)
B.3. PROJECT END DATE
USDA Pain
Level
Other (Specify :)
Anesthesia without surgery (i.e. blood
collection, gavage, etc.)
Paralytic Agents
Diagnostic X-rays
(B,C,D,E)
Antibody Production
Drugs (Drugs as research components other
than anesthetics, etc.)
. Immunization Procedures
Burns or Trauma
Biohazard
Food / Water Deprivation
Aversive Conditioning
Collection of Cells, Tissues, Organs
Prolonged Restraint
Non-survival Surgery
Est.
Proj.
Tot.
Multiple Survival Surgery
SPECIES (Common
Name)
Survival Surgery
For an estimated
number, please state how
many will be used for
biomedical use. For
others, state a desired
estimated number
realizing this could vary
greatly depending on
study type.
Special Diet
PROCEDURES
Check ALL that apply
C.1.
Pain level: BBreeding
only(No tail
nip); C-Only
momentary or
slight(needle
stick); D-More
than momentary
or slight but
relieved with
medication; EMore than
momentary or
slight and not
relieved
1
2
3
4
5
Other (Specify:)
D.1. Facility and Room # Where Procedures Will Take Place (If multiple, so indicate and if outside a facility, so state)
Non-Surgical Procedures
Surgery and/or Anesthesia
Euthanasia
Fish present a unique challenge for an IACUC because of the variety of fish and their use in research and
teaching.
To help determine if the use is legal and humane, please fill out the form which is divided into sections. More
than one section may apply. Please fill out all that apply.
D.2. In which category will the fish be used?
D.2.a. Aquaculture
D.2.b. Wildlife/Ecology
D.2.c. Biomedical
D.2.d. Teaching/Display
D.2.e. Behavioral Studies
D.2.f. Other
(Check all that apply)
D.3. What is the proposed source of the fish?
D.3.a. Commercial (e.g., Pet shop, another institution)
D.3.b. Wildlife
D.3.b.1. Are there other vertebrate species that could be clearly adversely
affected or become involved due to proximity?
If yes, please describe briefly, in your opinion,
how they might be affected.
D.3.b.2.Are there permits required for the use of these animals? (Federal,
state, local)
FAU Fish Protocol Page 4 of 16
Yes
Yes
No
No
If yes, include a copy of all permits for the
protocol when issued if applicable.
D.3.b.3. Are there permits required for the area (land, water, park, etc.)? )
If yes, include a copy of all permits for the protocol when issued if applicable.
If yes, as specifically as possible, describe
where the area is located that the research will
take place. (Attach a map if you have one.)
D.3.b.4. Will the environment be adversely changed by your research?
If yes, please briefly describe what you think will
occur.
If yes, as specifically as possible, describe
where the area is located that the research will
take place. (Attach a map if you have one.)
D.4. Where will the fish be housed?
D.4.a.
D.4.b.
D.4.c.
D.4.d.
D.4.e.
Justification for housing on
campus if from wild
Building/Location
Approximate duration in laboratory
How many times will the fish be
transported to the laboratory?
Who will provide the husbandry?
Room
D.4. Will there be a Quarantine/Prophylactic treatment of incoming fish?
D.4.f.
Describe quarantine plan including
special facilities, prophylaxis, etc…
D.4. Will there be Transport of fish?
D.4.g.
To ensure legal and safe transportation
of animals, please describe transport.
FAU Fish Protocol Page 5 of 16
PHARMACEUTICALS USED WITH ANIMALS
E.1. ANALGESIC/ANESTHETIC (Generic name)
SPECIES
DOSE MG/KG ROUTE FREQUENCY
SPECIES
DOSE MG/KG ROUTE
SPECIES
DOSE MG/KG ROUTE FREQUENCY
1
2
3
4
E.2. EUTHANASIA METHOD/DRUG (Generic name)
1
2
3
4
E.3. OTHER DRUGS(List all injected/given i.e., antibiotics,
fluids, Freund’s Complete Adj. etc.)
1
2
3
4
5
6
FAU Fish Protocol Page 6 of 16
F. INVESTIGATOR ASSURANCE
READ CAREFULLY AND SIGN AND DATE BELOW
PRINCIPAL INVESTIGATOR'S ASSURANCE
I have provided an accurate description of the proposed animal care and use protocol and agree to the following
conditions:
All personnel will be informed of the requirement to report fish related accidents.
All personnel will be informed that any concerns for inhumane care and treatment of fish or unlawful acts
involving fish should be reported to the IACUC or the Attending Veterinarian and that anyone reporting such
concerns cannot be discriminated against or be subject to any reprisal for reporting their concerns.
I agree to abide by governmental regulations and policies concerning the use of fish.
I will ensure that veterinary care is provided to animals showing evidence of pain or illness.
I agree to give consideration to tissue sharing and will do so whenever possible.
If the procedures concerning animal use in this research activity are to be revised or changed, I will so notify the
IACUC of these changes before the change is implemented. I understand that failure to request an amendment
for changes in animal use may place the University and myself in violation of Federal regulations.
As required by Federal regulations, I assure that the activities described do not unnecessarily duplicate previous
experiments and I assure the animal models proposed are the most appropriate for achieving the objectives of
this project and have provided justification for each model used in the protocol.
Principal Investigator Signature
Date
Requires a review of the protocol in a meeting with the veterinarian prior to submittal.
Attending Veterinarian’s Initials
Indicating that the meeting occurred.
FAU Fish Protocol Page 7 of 16
G. CONFIRMATION OF SCIENTIFIC/INSTRUCTIONAL MERIT REVIEW
Before any project that utilizes animals can be initiated, it must be reviewed and approved based on scientific or
instructional merit. The IACUC is responsible for ensuring a scientific or instructional review is conducted, not
necessarily to conduct the review. The IACUC is responsible for the proper, legal, and humane use of animals.
To assure the IACUC that this review is in place, the following information is needed regarding the review
process which is applicable for this protocol.
(Check one of the boxes below).
This project will only be initiated after it has been peer-reviewed outside Florida Atlantic University (e.g. NIH,
NSF, AHA, etc.) or within the University by a formal interdepartmental review group. If so, identify which group,
agency or board has reviewed or will review this project for scientific or instructional merit.
Name of Review Agency, Committee, or Board
Date of Review
OR
This project is being reviewed within FAU by one of several methods.
Departmental Committee for review of scientific or instructional merit
Name of Committee
Date of Review
Name of Committee Chairperson or Official Designee of the committee indicating approval
OR
Other Review Process
Describe the review process such as the IACUC, Department Chair person, etc.
Note: While it does not necessarily require someone who is an “expert” in the field of study, it does
require someone with sufficient scientific knowledge who can review a paper to ensure the project will
have merit and not use animals unnecessarily. Until a review is completed and the protocol
approved, the project can not be started.
Additional note: If the IACUC is asked to complete the scientific review, the project may be delayed until
someone with the required expertise can be recruited.
Name of person or persons who conducted the Scientific or Instructional Merit review.
If this is to be reviewed by the IACUC, then nothing will be required in this block since the IACUC will
have records of who reviewed the project.
Signature of Person
Date
In addition to any review within FAU, an “administrative review” by the chair person to indicate his/her
knowledge of the project and approval is required.
Signature of Department
Chairperson
FAU Fish Protocol Page 8 of 16
Date
H. PROTOCOL SYNOPSIS
Describe the proposed use of fish in non-technical terms. Briefly outline all procedures involving the use of fish and
other animals that will be affected. Focus on describing how animals will be monitored for pain and distress, and reduce
excessive detail on dosages and operative techniques. Include the following subheadings, as applicable: 1)
Goals/Rationale; 2) Quarantine/stabilization; 3) Procedures (with anticipated effects and what researcher and/or the
veterinarian will do in response); 4) Euthanasia; 5) Breeding. Safety of personnel and other animals should also be
discussed when applicable. If this is a teaching protocol, discuss how animals will be used in teaching.
NOTE: If this is a 3 year renewal, give a brief synopsis of study progress to date (research aims completed,
approximate numbers of animals used) BEFORE you discuss your current proposed work.
Justification (Include why these specific fish):
Quarantine/Stabilization (If applicable):
Procedure: (Be specific, what is being done to the fish, effect on the fish, pain and relief from pain or stress, etc.)
Breeding (If applicable):
Euthanasia:
Disposal:
Other:
ANIMAL RESEARCH and TEACHING PLAN
I. RATIONALE
Federal regulations and University policy requires that fish selected for a procedure should be of an appropriate,
minimum number required to obtain valid results and used at lowest pain/stress level.
I.1. Very briefly state the objective(s) and potential significance of the activities involving animal use
I.2. List each species selected and discuss its appropriateness as a research or teaching model.
(If wildlife, state species selected by [Agency] to be studied.)
I.3. Discuss the appropriateness of the NUMBER of animals to be used as follows:
State the number of animals needed for the total project. If the animals will be used for research, provide a justification, either statistical, quantitative,
etc. Even pilot studies, when selecting a number of animals (i.e. 3 or 6) should have a basis for its selection. (A suggested approach is to use a
statistical power calculation or a yield of tissue per organ).
Note: Wildlife studies. In wildlife study, just state the desired number to be studied and how you came up with that number, such as by an
agency’s request or the expected population available.
I.4. Summarize the experimental design in a simple table or other form that graphically clarifies how the groups,
time frames, and totals of animal use are broken down if possible.
FAU Fish Protocol Page 9 of 16
J. JUDICIOUS USE OF ANIMALS
Respond to all items. For all YES responses, provide descriptions and additional information in the blanks provided.
Attach additional sheets as necessary.
According to federal regulation (9CFR 2.31(e)(2), a proposal to conduct research or teaching utilizing animals "must
contain... a rationale for involving animals."
YES
NO
J.1. Are "whole live animals" required for this project rather than alternatives, such as cultured
cells? The questions the public may ask are: "Why do you need animals? Why can't you use a computer, tissue culture, etc.?"
If YES, include a brief explanation of efforts that you have made to reduce the number of animals used in
research (as it pertains to the 3 R’s [Replacement, Reduction, Refinement]) (Examples: “We will use the
minimum number requested by our agency”; “We have no control over the number caught but we will limit our
attempts to the minimum to give us the variety of fish we need. Care will be taken to safely release fish or
other species not required.”)
K. ANIMAL USE PROCEDURES
YES
NO
YES
NO
YES
NO
YES
NO
YES
NO
YES
NO
YES
NO
K.1. Blood sampling
(If YES, please discuss)
K.2. Urine, feces or other body fluids sampling
(If YES, please discuss)
K.3. Collection of tissues (except blood) BEFORE euthanasia
This would be filled out if animal tissues are collected prior to euthanasia. In some cases, the animal might be under anesthesia and might not be
allowed to recover. Antibody collection might be another example.
(If YES, please discuss)
K.4. Collection of tissues (except blood) AFTER euthanasia (If YES, complete the following)
This is the same as above except it is after death, thus lowering the chance of pain or distress to the animal. In some cases, tissues will be collected
both before and after euthanasia. If so, fill out both sections. The headings are the same as above
(If YES, please discuss)
K.5. In Vivo Administration of agents, other than anesthetics or analgesics, such as:
drugs/reagents/etc. (If YES, complete the following)
(If YES, please discuss)
K.6. Indwelling catheters or implants (If YES, complete the following)
(If YES, please discuss)
K.7. Will post-procedure care for non-surgical procedures listed above be required? (If YES,
complete the following)
K.7.a.
Who will provide care?
K.7.b.
What post-procedure care is required?
K.7.c.
When will post-procedure care be given?
K.7.d.
What analgesics will be given? (If none,
explain)
K.7.e.
What will be the endpoint?
L. ESTIMATION OF PAIN, DISTRESS AND SUFFERING
FAU Fish Protocol Page 10 of 16
The IACUC is required to review carefully any pain or distress involving animals. This section provides the required
assurance that you will be as humane as possible.
Federal law requires that the Principal Investigator consider alternatives to procedures that may cause more than
momentary or slight pain or distress to animals (Level D or E). A written narrative description of the methods and
sources (e.g. The Animal Welfare Information Center) used to determine that alternatives were not available is
required (Federal Regulations, 9 CFR Ch. 1, Sec. 2.31(d)(1)(ii)).
YES
NO
YES
NO
YES
NO
L.1. Does this project involve procedures that may cause more than momentary or slight pain or
distress to animals? (If YES, complete the following)
Database(s) searched -State the database(s)
L.1.a.
searched (e.g. MEDLINE, AGRICOLA).
L.1.b.
L.1.c.
L.1.d.
Date range of the Database Search
Date the Database Search was done
Key words - State the key words used to
determine that no alternative to procedures that may
cause more than momentary or slight pain or distress
to animals is available.
L.1.e.
Please provide a brief narrative description of
search results that led you to believe that there
are no alternatives to procedures that may cause
more than momentary or slight pain or distress to
animals (this section does not refer to
“alternatives” to animal use) A simple statement of what
you found from your literature search (e.g., "From my literature search, I
found no alternative for the use of procedures that may cause more than
momentary or slight pain or distress to animals.").
L.2. What pain or distress is anticipated? (Be explicit and
include potential for pain or distress (in the presence or
absence of anesthesia) Fully describe the pain or distress you expect to
occur or has the potential to occur during all procedures. In most cases, a simple
statement that “The animal will only perceive the momentary pain associated with a
needle stick” or Pain associated with the surgery will be alleviated by anesthetics
and analgesics.”
The PI, the project personnel and Comparative Medicine observe for the following signs of pain or distress:
Loss of appetite
Loss of weight
Loss of mobility
Restlessness
Abnormal postures
Skin disease
Fighting
L.3. Are there other signs that will be used to assess pain and distress? (If YES, explain below)
L.4. For protocols that have procedures that are potentially more than momentarily painful or
distressful, special observation is generally necessary. Does this protocol include such
procedures? In many cases, the correct answer will be NO. If an animal is just euthanized for tissue collection, this should be marked NO.
Any time more than momentary pain or distress will be expected for the animals, the answer has to be YES
L.4.a.
Frequency of observation How often will the animal be observed? The frequency will
depend on the amount of pain or distress. If in doubt, check with the University Veterinarian.
Whatever you put in this box needs to be documented and submitted on request by the IACUC,
Veterinarian or External Reviewers; if you will observe the animal “daily” you need to have records
that include documented weekend checks.
L.4.b.
Beginning (date or condition) The period when the animal will be checked has to be
defined. It generally starts just before the time you expect to see signs of pain or distress.
L.4.c.
Ending (date or condition) This is to ensure the animal will be properly observed until the
pain or distress is ended
FAU Fish Protocol Page 11 of 16
YES
NO
L.5. Will there be unalleviated pain or distress in this protocol?
(If YES, this protocol falls into the USDA pain level E. Please supply the scientific justification why animals on this
protocol will be subjected to unalleviated pain or distress and enter USDA code E. The law states that any procedure that could cause
more than momentary pain or distress requires relief, generally through analgesics. The University Veterinarian is not allowed to diverge from the law. It is
assumed that an animal that has undergone surgery has pain post-surgically. Other procedures may also cause pain. Such a procedure is usually evaluated as to
how painful or distressful it would be to a human, and appropriate analgesia has to be provided. If USDA level "E" is specified, therefore, no drugs are to be used
and a full justification is required.
FAU Fish Protocol Page 12 of 16
M. STUDY TERMINATION/ANIMAL DISPOSITION
There are three possible outcomes for an animal assigned to a protocol:
1. Absolutely no harm to the animal will occur, and the animal will be available for redistribution
2. The animal will be euthanized at the end of the research
3. The animal will be in the experiment until it dies (commonly referred to as “death is the endpoint”)
YES
NO
YES
NO
YES
NO
YES
NO
YES
NO
YES
NO
M.1. Will any animal(s) survive the research with no harm?
(Often yes for Agricultural, Teaching, and Wildlife protocols) Examples of "no harm" include only small amounts of blood drawn or a diet study.
Complete M.1.a. and M.1.b. if “Yes” only
M.1.a. Will any animal(s) be used in another protocol?
(If YES, indicate protocol # and PI)
Protocol #
PI
M.1.b. Will any animal(s) be adopted?
(Rarely done but if the answer is YES, contact the University Veterinarian to make
arrangements.)
M.2. Will any animal(s) be euthanized?
M.3. Will death be the endpoint for any animal(s) in this protocol? ( i.e. The animal’s death is a
required experimental data-point.)
(For example, a disease is introduced and the fish have to progress to death to see the final pathology).
(If YES, complete the following)
M.3.a. Will euthanasia ever be considered or is there any other measurable endpoint at
which euthanasia will be performed? If YES, this may not be death as an endpoint;
ONLY COMPLETE THIS SECTION IF M.3. is “YES”
Please explain
M.3.b. If euthanasia will never be
considered, please justify why death is
the end point rather than euthanasia
M.3.c. What signs are the animals
expected to exhibit as they go through the
terminal stages?
M.3.d. What measures can be taken to
alleviate pain (e.g. analgesics)? (If NONE,
please justify)
M.3.e. Who will observe the animal during
the terminal stages?
FAU Fish Protocol Page 13 of 16
M.4. Instructions for disposition of sick or injured animals.
This section is used to indicate what is to be done with animals in case they become sick or injured and what is to be done with dead animals and
tissue. (Weekend, holidays and nights are times that animals need care too, e.g., without being able to contact the investigator, the animal caretaker
may not know whether or how to keep the carcass until it can be examined or utilized by the investigator). An alternate person should always be
designated. The alternate person cannot include routine CM animal caretakers and that person should also be listed under the project personnel.
M.4.a.
YES
NO
YES
NO
YES
NO
YES
NO
Call investigator Immediately? (A “no” answer means the investigator will be called the next
working day.)Normally, the investigator should be called. The investigator may wish to be notified the next working day;
complete I.5.b.
M.4.b.
In case of emergency, should the Veterinarian treat the animals? In case of an emergency, the
veterinarian needs to know whether the animal should be treated. If the animal should not be treated, why not? Should the animal
just be euthanized? If the animal is to be treated, what restrictions, if any, should the veterinarian be aware of (i.e. no steroids; no
drugs; etc.)
If NO, include justification on why the animal will
not be treated.
If YES, explain any restrictions on treatment below.
M.4.c.
Comments
M.5. Instructions for disposition of dead animals.
M.5.a.
Call investigator Immediately? (A “no” answer means the investigator will be called the
next working day.)
M.5.b.
Do you specifically request the Veterinarian to do a necropsy? The veterinarian is required to be notified
of any unexpected death and will either perform or be present at the necropsy. When deaths occur that are expected, the
veterinarian does not need to be notified, but if the death occurs after normal hours, or if the investigator or his/her backup cannot
be reached, the veterinarian may be asked to try to save certain tissues. In such circumstances, what instructions would you have
for him/her?
Discuss any special instructions for the
Veterinarian. (i.e. look at gills, save heart,
save implant)
Other Areas
O. Are there any known chemical, physical, or other hazard involved?
(If YES, please discuss-include waste water,
the use of MS222, etc,)
P. Is there prolonged restraint involved?
(If YES, please discuss)
Q. Are there any diet deviations from standard?
(If YES, please discuss)
R. Is there anesthesia used to relief pain?
(If YES, please discuss)
S. Is there any surgery involved?
(If YES, please discuss)
T. Behavioral
(If YES, explain if this is observation,
positive/negative behavioral modification,
etc.)
Breeding Colony
(Skip to next sections if not applicable)
If a breeding colony is deemed appropriate, it is the investigator’s responsibility to breed only enough animals
necessary to complete their research. Excess production of animals that are then destroyed is not in parallel with the
University’s commitment to “Reduce”, “Refine” and “Replace” the use of animals in research.
U.1. What species, stocks and/or strains will you be breeding?
U.2. Why are you breeding fish?
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U.3. Describe very briefly how you plan to care for the
fish. Include diet, observation, water condition and any
other factors the IACUC needs to know about to ensure
proper care.
U.4. Describe how you plan to terminate the project.
Include euthanasia and disposal of fish and water.
Aquaculture
(Skip to next sections if not applicable)
V.1. Will your research affect the performance or survivorship of the fish being studied?
If yes, please briefly describe what you think will occur.
V.2. Will any procedure, intended or that might be necessary to be used, involve restraint, diet
changes, anesthesia or surgery?
If yes, please briefly describe.
V.3. Describe the outcome for the fish. Include how and
where released, euthanasia or other outcome, and what
would decide each.
V.4. Discuss any other information that would help the
IACUC to approve this project.
Biomedical
(Skip to next sections if not applicable)
W.1. Will your research affect the performance or survivorship of the fish being studied?
If yes, please briefly describe
what you think will occur.
W.2. Will any procedure, intended or that might be necessary to be used, involve restraint, diet
changes, anesthesia or surgery?
If yes, please briefly describe
what you think will occur.
W.3. Describe the outcome for the fish. Include how and
where released, euthanasia or other outcome and what
would decide each.
W.4. Discuss any other information that would help the
IACUC to approve this project.
YES
YES
Teaching/Display
NO
NO
(Skip to next sections if not applicable)
Yes
No
X.1. Are fish just used for display only? (If yes, you are finished.)
X.2. Are fish used for teaching?
Complete only if applicable to your project. Additionally, all individuals that will be involved with the
animals are required to receive training. Contact Veterinary Services to discuss training.
A training protocol is used to train individuals in a particular procedure requiring a live animal. With all the new mechanical and computerized
training devices now on the market, the use of animals has decreased for training purposes. There are still specific programs that require the use
of live animals. Please fill out the following if you plan to use animals in teaching.
Please provide a list of the students involved in the course to the IACUC after each class and discuss with the veterinarian how student training
for the use of animals will occur.
Check all that apply.
Undergraduate students
Graduate students
Continuing education students (MD, DVM)
BioMed Course #
Zoology/Biology Course #
Other Course #
Only dead animals or tissues obtained through euthanasia by the PI
Non-survival surgery (fill out the SURGERY FORM)
FAU Fish Protocol Page 15 of 16
Demonstration only by PI
Student involvement - live animal observation and handling
Student involvement - exposure to research
Student involvement - gain skills, more than just handling
(Explain)
Other (Explain)
FAU Fish Protocol Page 16 of 16