Standard Operating Procedure
Animal Biosafety Level 1
Complete all highlighted sections and e-mail to animal.hazmat@ucdenver.edu
Date and IACUC #
PI and PROTOCOL
SPECIES/STRAIN:
HAZARD CLASS
PI enter information
POTENTIAL CLINICAL SIGNS
OF EXPOSURE
PERSONAL PROTECTIVE
EQUIPMENT
WHAT TO DO IF YOU THINK
YOU HAVE BEEN EXPOSED:
HOUSING:
CAGE HANDLING
GENERAL HOUSEKEEPING
ABSL 1 SOP
Date:
PI name and Protocol #:
List Species & Strain:
Biological Materials to be administered:
Dose & Route:
Excreted:
IACUC #:
A Biological Dose Card (no special handling) must be placed on each
cage as animals are dosed.
PI enter information for review by Biosafety Office:
Standard PPE: gown or scrubs; gloves, shoe covers, hair cover, mask.
STANDARD PRECAUTIONS AND BSL2 PRACTICES FOR
ADMINISTRATION OF HUMAN MATERIALS ARE REQUIRED. SHARPS
PRECAUTIONS ARE REQUIRED.
YOU MUST WASH YOUR HANDS upon removing gloves and other PPE,
after working with animals or their housing units.
IF YOU THINK YOU HAVE BEEN EXPOSED:
1) Immediately wash the exposed area with soap and water.
2) Eye wash stations are located in every procedure room, flush eyes
for 15-20 minutes.
3) Report the exposure to your supervisor.
4) Report immediately and seek medical attention from the Workers
Compensation provider during regular business hours or to the
University of Colorado Hospital Emergency Room if the exposure
occurs after hours.
All exposures must be reported to the Biosafety Officer, the Facility Manager
and documented with a workers compensation claim form on the University
Risk Management website: http://www.cu.edu/content/fileclaim
Standard ventilated rack housing, unless otherwise discussed in collaboration
with OLAR and EHS due to research needs
Cages will only be handled with gloved hands. Cages will only be opened and
animals manipulated in the Biosafety Cabinet (BSC) or Animal Transfer
Station (ATS). Do not use ATS to administer agents to animal subjects. All
cages will be marked with a Biological Dose Card (no special handling) as the
animals are dosed. Animal Care Staff are responsible for the husbandry
practices.
The work surfaces of the biosafety cabinet (including control buttons) must be
disinfected before and after cage changing and all other animal manipulations.
Follow all sharps and Standard Precautions. Red sharps containers will be
provided by animal care staff for proper disposal (autoclaving) of sharps items.
Do not recap sharps.
1
rev November 26, 2014
WASTE DISPOSAL
Sharps are collected in appropriate sharps containers. All other wastes
generated as a result of dosing the animals, must be placed in a red regulated
medical waste bag. The bag will be tagged with the originating housing room
number. The exterior of the bag will be sprayed with a disinfectant prior to
transport.
Regulated Medical Wastes including sharps containers are transported to
designated CCM collection point. Designated CCM staff will transport to EHS
collection point for proper disposal.
The Investigator must be notified by telephone or e-mail of animals found dead or
near dead.
ANIMAL CARCASSES
Principal Investigator
Contact Phone No::
Email:
Protocol number:
Alternate Contacts:
Carcasses with PI enter information on materials are not permitted to enter the
Raptor Program. Once the carcass bag is closed, the exterior of the bag is
sprayed with disinfectant. All carcass bags must be labeled with the PI name
and the housing room number. Carcasses will be placed in the designated
refrigerator/freezer.
PI enter information:
PI enter information:
Environmental Health & Safety
Phone: 303-724-0345
Biosafety Program: 303-724-7395 or 303-724-5954
Chemical/Hazardous Materials Program: 303-724-0344
EH&S website: www.ucdenver.edu/ehs
ABSL 1 SOP
2
rev November 26, 2014