Name: ________________________________________________Block: ______ Date: _________ How Does DNA Direct Protein Synthesis? Introduction Protein synthesis is a complex process. In this activity you will trace the steps that are involved in protein synthesis. Keywords DNA mRNA tRNA rRNA transcription translation amino acid protein synthesis Procedure 1. DNA is too large to leave the nucleus through the nuclear pores. Instead, it serves as a template for messenger RNA (mRNA) to copy the code and transfer it to the ribosome. This process is called transcription. Write the sequence of mRNA codons that would result from the transcription of this portion of DNA. DNA: TAC ATA TAG CTT TTG ACG GGG AAC CCC ATT mRNA: ____ ____ ____ ____ ____ ____ ____ ____ ____ ____ 2. After transcription, mRNA leaves the nucleus and attaches to a ribosome. The ribosome helps to join the corresponding transfer RNA (tRNA), which carries a single amino acid determined by the anticodon. Recall from biochemistry amino acids are the building blocks of proteins. As tRNA carries the amino acids to the ribosome the polypeptides are synthesized based on the code sequence carried on on mRNA. This process is called translation. The tables show the mRNA codons and the amino acids for which they code. For example, if you were given the mRNA codon AGA, you can see on the table that these bases code for the amino acid Arginine. Use the mRNA sequence above to write the sequence of amino acids carried by the tRNA. Amino Acids: __________, __________ , __________ , __________ , __________, __________, __________ , __________ , __________ , __________. Questions 1. How many amino acids make up the portion of the newly synthesized protein from the above question? ____________ 2. What is the purpose of the UAA codon? 3. What do the letters RNA stand for? __________________________________________________________________ 4. How is mRNA different from DNA? ___________________________________________________________________ _________________________________________________________________________________________________ 5. On mRNA, what nitrogen base binds with the DNA base: A?______ G?_____ T?______ C? ______ 6. Where in the cell is mRNA made? ___________________________________________________________________ 7. What enzymes are used in the process of transcription? __________________________________________________ 8. What carries the amino acids to the ribosome where it joins with the mRNA? __________________________________ 9. How are mRNA and tRNA alike? ____________________________________________________________________ _________________________________________________________________________________________________ 10. How are mRNA and tRNA different? ________________________________________________________________ _________________________________________________________________________________________________ 11. What determines what proteins are made? ___________________________________________________________ Label the diagram: A. _________________ B. _________________ C. _________________ D. _________________ E. _________________ F. _________________ G. _________________ Online Animations & Videos: Sign up, log in and click on the RNA Protein Synthesis Links and explore transcription and translation. Web Page 1: Read, think about the diagrams and study the process. o http://www.wisc-online.com/objects/ViewObject.aspx?ID=ap1302 Web Page 2: Practice the process of protein synthesis. Be sure to read the bold text below the diagram for instructions on what to do! It does not take long so you might want to try a couple of times to be sure you know how to transcribe and translate the DNA sequence into a protein. o http://learn.genetics.utah.edu/content/begin/dna/transcribe/ Web Page 3: Khan Academy has updated a lot of their lessons and videos. Check this one out to help you further understand this complicated process. o https://www.khanacademy.org/partner-content/nova/rnawondermolecule/v/proteinsynthesis