.
UNIVERSITY OF PENNSYLVANIA
Location:
Tel:
Fax:
Website:
Mailing Address:
D2 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104-6085
D2 Richards Building
215/898-2795
215/573-9327
http://www.med.upenn.edu/genetics/cellctr/services.shtml
_________________________________________________________________________
REQUEST FOR HYBRIDOMA GENERATION (FUSION)
User Name: __________________________________________ Penn Key_____________________________________
Principal Investigator: _________________________________ Fund No/ PO No: ______________________________
Date: _______________________________________________ Tel: __________________________________________
Email: ______________________________________________ Fax: _________________________________________
Prices subject to change. Contact Cell Center Services for current prices.
Antigen Information:
Antigen Name:______________________________________________
Is Antigen:
A Protein
A Peptide
Antigen concentration and amount: ____________________________
Antigen size: ____________________ kD
In case of Peptide: Is the peptide conjugated to a carrier protein? If yes, which carrier? _____________________________
Please check the requested service(s)
Peptide conjugation to KLH carrier protein
Immunization:
Fusion:
number of mice _____________ (the one mouse with the best titer will be used for fusion)
number of 96-well plates cells will be plated into ______________
Fusion maintenance and harvesting of supernatants for testing
Screening of supernatants by ELISA
Expansion and freezing of positive clones:
Subcloning by limiting dilution:
number of clones _____________
number of clones ___________________
Staff Use Only
Start Date _______________________
Revised 02072012
Completed ______________________
By: _________________________