SSRL MACROMOLECULAR CRYSTALLOGRAPHY PROPOSAL FORM Return 1 electronic copy to Lisa Dunn, SSRL, 2575 Sand Hill Rd., Menlo Park, CA 94025, (650) 926-2087, lisa@ssrl.slac.stanford.edu Applications may be screened by a number of SSRL scientific staff for scheduling and safety related issues . SUBMISSION INFORMATION: Date: Proposal Number (if Extension/Replacement) Check if Applicable: __ Extension/Replacement __ Monochromatic __ MAD / SAD __ Ultra-High Resolution (≤ 1 Å) __ Remote Access Data Collection Will proprietary research be performed under this application?** (Note that proprietary research is subject to specific terms and conditions, and SSRL must be reimbursed at full cost recovery) Yes No AIDS Related? Yes No (underline choice) *http://www-ssrl.slac.stanford.edu/users/user_admin/px_proposal_guide.html TITLE OF PROPOSED EXPERIMENT: MANDATORY: Please write a short 8-10 line abstract. Note: this abstract will be made publicly available on the NIH database as part of our grant reporting requirement. NAME OF SPOKESPERSON (First, MI, Last): DEGREE: INSTITUTION ADDRESS: OFFICE/LAB PHONE(s): E-MAIL ADDRESS: CITIZENSHIP: PRINCIPAL INVESTIGATOR(S) AND SOURCE OF RESEARCH FUNDS SUPPORTING THIS PROJECT: 1 CRYSTAL DATA: please complete for each sample you plan to bring Mol. Wt. Size Sample(s): (mm x mm x mm) (kD) Cell Max Space Dimensions Res Group (Å x Å x Å) Temp* NCS** (Å) Anom Scattering Wavelength Element Anom Sites/ Molecule * Temperature of Data Collection ** NCS: Number of molecules expected per asymmetric unit MANDATORY: For each project/sample please give a short description (usually 1-3 pages) of what the planned work includes, why the structural work is significant, and a clear statement of need for synchrotron radiation. This statement, and the crystal information given above, will form the basis for deciding what priority each proposal will receive. If the proposal contains multiple projects, please continue on separate page(s). If the proposal is a extension please 1) be clear on what was accomplished earlier and why additional time is needed, and 2) complete the data summary forms (page(s) 4) as well. PUBLICATION INFORMATION: HAVE YOU RECEIVED BEAM TIME AT SSRL IN THE PAST? YES NO IF YOU HAVE PREVIOUSLY RECEIVED BEAM TIME AT SSRL, HAVE YOU NOTIFIED SSRL OF ALL OF YOUR SSRL RELATED PUBLICATIONS, PATENTS AND/OR AWARDS? YES NO (If not previously reported, list below or append to this proposal information on publications, patents, and awards that resulted from your prior beam time at SSRL (refer to lists at www-ssrl.slac.stanford.edu/pubs/): IF YOU PUBLISHED RESULTS RELATED TO YOUR SSRL BEAM TIME, DID YOU ACKNOWLEDGE SSRL AND FUNDING SOURCES IN THESE PUBLICATIONS? YES NO (If not already done, please refer to instructions and sample acknowledgements at www-ssrl.slac.stanford.edu/pubs) 2 DO YOU HAVE ANY SUGGESTIONS OR CONCERNS WHICH YOU WOULD LIKE TO SHARE WITH SSRL, THE PROPOSAL REVIEW PANEL, OR THE SSRL USERS' ORGANIZATION EXECUTIVE COMMITTEE? COLLABORATOR(S) INVOLVED IN THE EXPERIMENT: Name (First, MI, Last) Degree (Remote, Institution (if different from above) E-Mail Address Citizenship Onsite, Both) POTENTIAL SAFETY CONCERNS OR HAZARDS ___ No hazardous substances, equipment, or procedure will be brought to SSRL as part of this proposed experiment. IF YES, please complete safety questions below. Additionally, provide detailed safety procedures in proposal text. CHEMICAL USE? ( ) No ( ) Yes Substances:_____________________________________________________________ Common Names:________________________________________________________ Note: If you are bringing a Hampton Kit for heavy atom derivatives, please list catalog # _____________ BIOHAZARDOUS MATERIALS USE? ( ) No ( ) Yes - If yes, what type __________ & what is the materials NIH classification _________________ HUMAN OR ANIMAL SUBJECT USE? ( ) No ( ) Yes - If yes, what type __________. *Please contact SSRL Safety Office for further guidance. RADIOACTIVE MATERIALS USE? ( )No ( )Yes* - If yes, what is the materials specific activity ________________________________________ LASER USE? ( ) No ( ) Yes - If yes, ANSI classification: ___________ _Wavelength: _________ Laser hazards controls you will apply: 3 Total power: __________ FOR SSRL FEDERAL AGENCY REPORTING PURPOSES, PLEASE CATEGORIZE YOUR PROPOSAL: RESEARCH AREA (type X in cell to left of all that apply) Materials Science Polymers Earth Sciences Engineering Physics Medical Applications Environmental Sciences Instrumentation or Other Development Chemistry Biological and Life Sciences Optics Purchase of Specialty Services or Materials Other: (specify) FUNDING AGENCY (type X in cell to left of all that apply) DOE/BES NASA USDA Fdn/Research Inst. DOE/BER NIH Homeland Security State/County/City DOE Other: (specify) NIST Other US Gov’t: (specify) Prof/Voluntary Assoc. DoD: (specify) NNSA Industry Foreign: (specify) DVA Other: (specify) NSF 4 EXTENSION APPLICATION FOR SSRL MACROMOLECULAR CRYSTALLOGRAPHY PROPOSALS THE FOLLOWING INFORMATION MUST ALSO BE COMPLETED IF THIS APPLICATION IS AN EXTENSION PLEASE COMPLETE FOR DATA COLLECTED AT SSRL (Make additional copies as necessary) BL1-5 BL7-1 BL9-1 BL9-2 Sample* Wavelength Used Date Collected Number of 8-Hour Shifts Used Temperature Space Group Resolution (Å) Total Number of Reflections Number of Unique Reflections Rsym (I) Rsym in Highest Res. Shell % Completeness Helped Model Building? Other Relevant Information * Be sure to describe on page(s) 2 how data were used. Please define the sample name in the narrative. LIST PUBLICATIONS BASED ON WORK DONE AT SSRL ON THIS PROPOSAL: 5 BL11-1 BL11-3