Biology 357 - Ecology Name:______________________________________ Laboratory Day and Time:______________________ Ecology Laboratory #1 “The Art of Scientific Writing, Thinking and Bias” (30 points - due in two weeks) INTRODUCTION As a student in this course, your primary objective is to place yourself in the position of a scientist. This requires learning a great many things: how to think, collect data, and draw informed conclusions from your work. Part of the process also includes producing reports so that your fellow scientists know and repeat your work. This is an essential element of the scientific process because without it, knowledge is neither disseminated nor gained. Your objectives today are to briefly learn about the scientific method, data collection and analysis, and scientific writing. You will have to write a scientific report, according to the guidelines, and submit it in two weeks. This lab report is worth 30 points. I will grade you on spelling, grammar, how closely you follow the required format, and what you say. PART I: THE SCIENTIFIC METHOD Most of us think about different things when we think about the scientific method. You may have been exposed to it during other courses during your high school and college careers (so far, that is). If you haven’t, then here it is and I hope it isn’t too confusing. It’s really a very simple and straight-forward process to follow when answering a scientific question. It begins with a simple observation and proceeds through steps to lead you to a conclusion. Here are a list of the steps, reviewing each of them in class: 1. 2. 3. 4. 5. 6. 7. An observation. A question? A hypothesis (a guess). An experimental design to test the hypothesis. Collecting the data. Analyzing the data (with the help of statistics). Accept or reject your hypothesis based upon your analysis. Scientists follow this process because it uses logical deduction to test ideas and draw logical conclusions. In today’s lab, the observation involves the simple flip of the coin, and how to test various ideas about it. For example, why would we, or anyone for that matter, be concerned the simple flipping of a coin? Well, think about some examples where a coin flip might be used. 1. 2. Sporting events. Gambling (illegal and legal forms). Why flip a coin under these circumstances (answer the question below)? Biology 357 - Ecology 2 Once you have done this, you have a pretty good idea as to why and forms the basis for your question (?). The question takes your simple observation and places it within the context of the scientific process. Most of the time this step is completed in one’s own head without the benefit of writing it on paper. But it helps you move to the next step: the hypothesis. However, you have to refine this hypothesis into a usable form. To do this, you have to write your hypothesis into 2 types: a null hypothesis (no difference), and an alternate hypothesis. We will learn the process of developing a correct hypothesis in class. However, participate in class and write down the correct null and alternate hypotheses in the spaces below when the class is finished. Our null (“no difference”) hypothesis: Our alternate hypothesis: When the null and alternate hypotheses have been written, you will be better able to design an experiment to test it. We will discuss it in class, but most of the directions are listed on the following pages. I’ve also included a sheet to record the data as you collect it. Directions: Data Collection 1. 2. 3. 4. Work with other students in the lab. Write your names on the data sheet. Decide which one of you will “flip” the coin, while the other records the data. Flip the coin using the same method every time. Flip it 250 times and record your results in the table by checking the appropriate box. When you are finished, tabulate (summarize) your results at the bottom of the table. Data Analysis 1. 2. You have a sample size of 250 “flips” Sample size is indicated with a small letter “n” (n = 250) to indicate an estimate instead of a known entity. However, what do you do next to determine if the coin was biased? If the coin was unbiased, and you flipped it 250 times, how many times would you have expected to tails or heads? Write your answer here: # Tails # Heads But,...... 3. What if the result was different than what you expected? If you found a difference, then how much of a difference is needed for you to conclude that you or the coin is biased towards heads or tails? Biology 357 - Ecology 3 Fill in the blanks below: Number of heads/250 flips: Number of tails/250 flips: Number of heads/250 flips you expected if the coin was unbiased: Number of tails/250 flips you expected if the coin was unbiased: 4. Take the number of tails you found, and subtract the number expected tails. 5. Square that result (multiply it by itself). 6. Divide it by the number of tails you found (use the number again). 7. Record that number here: 8. Now repeat that process for the head and record the result below. Tails: Heads: 9. Add the 2 results. Write your result here. Bring your result to me and I will be able to tell you if your coin is biased. Thus, you have completed data analysis and you must either accept your null hypothesis, or reject your null hypothesis. If you reject your null hypothesis, then, by deduction, you accept your alternate hypothesis. Next, as a member of the scientific community you are responsible for telling everyone about it. A scientific report effectively communicates your work to other scientists. PART II: WRITING THE SCIENTIFIC REPORT Writing a scientific report is step-by-step process of informing the reader what you did, how you did it, and what you concluded. In this class, you have to write a report of your laboratory research. This allows us the opportunity to read about your work and repeat your experiment if we choose. Each scientific report is composed of several sections: 1. 2. 3. 4. 5. 6. 7. 1. Title Abstract Introduction Methods and Materials Results Discussion and Conclusions Literature Cited/References Title A title informs the reader about your work. It is meant to grab your attention and invite you to read the article. A title is not more than 10 words. A vague or inaccurate title can waste a reader’s time by suggesting, erroneously, that the paper contains certain information. Even worse, Biology 357 - Ecology 4 a good paper can be burdened by a bad title and may not catch the eye or interest of its intended audience. Some authors initially create a working title first and then revise it upon completion of the paper. Others wait until they finish the paper before writing the title. Thus, they have a better understanding of the paper;, the title will reflect this greater understanding. 2. Abstract The abstract is a brief review of the article and is meant to provide brief elements of the paper so that the reader can quickly gain a better idea of the entire article. It tells the reader what you did, why you did it, what you found, what type of statistics you used, and what your results mean in a larger context. 3. Introduction Begin by clearly identifying the subject and nature of the laboratory exercise. State the hypotheses and/or define the problem your research was designed to solve or, in this case, the course concept to be understood. Orient the report to previous stated concepts in the lecture and textbook, possibly by a brief and concise review of the literature that is unmistakably relevant to the specific aims of your research. Do not use the Introduction to try to convince the reader of the importance of the report, it should be evident. Also, a major problem for most writers can be linking the paper to a subject’s broader conceptual framework. With practice this will disappear, as experienced writers automatically link the study to the conceptual framework. 4. Methods and Materials Describe materials and methods used, including experimental design, in sufficient detail to enable other scientists to evaluate your work or to duplicate your research. The usual sequence for experimental studies is design of the experiment, subjects (plant, animal, human), materials used, procedures, and methods for observation and interpretation. Your methodology provides the context for evaluating the data. The credibility of your scientific argument depends, in part, on how clearly and precisely you have outlined and justified your procedures. Organize your material logically, use specific, informative language, and omit unnecessary information. 5. Results The results section should: (1) summarizes the data, emphasizing important patterns or trends, and (2) illustrate and support your generalizations with explanatory details, statistics, examples of representative (or atypical) cases, and tables/figures. Integrate your graphs and figures with the text to explain your results. To convey the results clearly, your writing must be wellorganized. Present the data in a logical order, if possible, in the same order you presented the materials section. This is called parallel construction. If the Results section is long and includes many different topics, consider using subheadings to make the text easier for the reader to grasp. The Results section should be a straightforward report of the data. Do not compare your findings with those of other researchers, and do not discuss why your results were or were not consistent with your predictions. Avoid speculating about the causes of particular findings or Biology 357 - Ecology 5 about their significance. Do not interpret the data or draw major conclusions. Save such comments for the Discussion. 6. Discussion In the Discussion, tell the reader what your think your findings mean. Do the data support your original hypotheses? Why or why not? Refer to the graphs/figures in the Results; use them as evidence to support your conclusions. Here is the place, too, to discuss the work of other researchers – your ability to apply to a broader context and conceptual framework. Are your findings consistent with theirs? How do your results fit into the bigger picture? Do not present every conceivable explanation. Sometimes beginners feel obliged to think of every possible way to interpret their results. However, you can quickly swamp and lose a reader. Experiments do not always have to confirm the presence of major differences. You may find that there is no significant difference between two groups, no effect, and no association. These results are important and worthy of explanation. Show that you are knowledgeable about the subjects and take responsibility for your conclusions. Don’t hedge or hesitate and use coherent, logical organization in your writing. Finally, discuss these two questions in the Discussion: A. Notice the variation in the results versus the total counts. Regardless of the results (biased or not), what factors do you think could account for the result? B. What do you think would happen if we combined your results with the results from other classes? Would the results change or remain the same? 7. Literature Cited A bibliography contains all the sources mentioned in the text, along with additional references on the topic. The Literature Cited section of a paper contains only the literature (sources) that have been cited in the text. Even if you have acquired useful background knowledge by reading five articles and three books, do not list any of these in the Literature Cited section unless you have specifically mentioned them in the text. Make sure that you report your sources completely and accurately. If your sources are reported sloppily, people may doubt your authority, integrity, and thoroughness as a researcher and writer. I will assist you in this section. Journals have adopted various formats for the Literature Cited section of papers. Prospective authors prepare this section by carefully following guidelines prescribed by journal for which they are writing. The following rules illustrate the style used by the journal American Naturalist. Example - Journal Article with Single Author Grant, P. R. 1995. Speciation and the adaptive radiation of Darwin’s finches on the Galapagos Islands. Am. Sci. 69:653-663. Type the author’s last name first, followed by his or her initials. Give the publication date next, then the title of the paper. Only the first word of the title is capitalized. The title of the Biology 357 - Ecology 6 journal is capitalized but not italicized. Note that the title is abbreviated to save space and printing costs. After the journal title, give the volume number followed by a colon and the pages on which the article appears. Arrange your references alphabetically and by year. PART III: THE SCIENTIFIC REPORT FORMAT You must you format your report according to specific guidelines. They were presented during lecture for the mid-term examination and must be repeated here: 14 October 2008 Jeffrey W. Snyder Department of Biology Western Oregon University 345 N. Monmouth Ave. Monmouth, OR 97361 E-mail: snyderj@wou.edu WRITING AND SUBMITTING LABORATORY REPORTS JEFFREY W. SNYDER, Department of Biology, Western Oregon University, 345 N. Monmouth Ave., Monmouth, OR 97361, USA JOURNAL OF ECOLOGY 00(0):000-000 Key words: reports, writing, laboratory, title, abstract, introduction, methods, results, discussion, literature cited. Title Abstract Introduction Methods and Materials Results Discussion and Conclusions Literature Cited/References Word Count: 722 words LITERATURE CITED McMillan, Victoria. 1988. Writing papers in the biological sciences. St. Martin’s Press, Inc. Boston. 142 pp. Day, Robert. 1994. How to write and publish a scientific paper. Oryx Press, Inc. Phoenix, AZ. 4th Edition. 223 pp. Trimble, John R. 2000. Writing with style – conversations on the art of writing. Prentice-Hall, Inc. New Jersey. 198 pp. Stegner, Wallace A. 2010. Creating a society to match our scenery: resolving natural resource conflicts in the 21st Century. Journal of Ecology 32:100-101. Biology 357 - Ecology 7 PART IV: GRADING THE LABORATORY REPORT Examination Grade Sheet Student’s Name: . Date Received: 1. Laboratory#: Penalty Points: 1 . 0 Style and Format Followed specified journal format guidelines (5 points) 2. 3. 4. Grammar (2 points) _______ Spelling (2 points) _______ Punctuation (1 point) _______ Literature Citations Used appropriate document background (5 points) _______ Used appropriate citation format in text (2 points) _______ Used appropriate citation format at end of text (2 points) _______ Tables, Figures and Data _______ Content and Integrative Thought Content and Synthesis – covered most points (5 points) 6. _______ Grammar, Spelling, and Punctuation Data presentation (5 points) 5. . Did You Staple This Grade sheet to the Paper? (1 point) Total Points (30 points) Late Penalty Deduction (1 point/day): Your Preliminary Score: Comments: _______ _______ _______ ___0___ / 30 . Biology 357 - Ecology 8 PART V: DATA SHEETS Student #1 Name: Student #2 Name: Student #3 Name: Student #4 Name: Biology 357 - Ecology Sample 1 2 3 4 5 9 Heads Tails Sample Heads Tails Sample 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 Totals (f i) Totals (f i) Totals (f i) 6 7 8 9 10 11 12 13 14 15 Heads Tails Biology 357 - Ecology Sample 10 Heads Tails Sample Heads Tails Sample 121 161 201 Totals (f i) Totals (f i) Totals (f i) Heads Tails