ABSTRACT The study was designed to evaluate the effect of different rates of glyphosate on different ages of spear grass regrowth. The trial was laid out in a randomized complete block design with a split plot arrangement and replicated three times: The main plot consisted 5 slashing regimes which include no-regrowth, 2weeks regrowth, 3weeks regrowth, 4weeks regrowth and no- slashing, while sub-plots consisted of 5 rates of glyphosate applied at the rate of 3.76kgai/ha, 2.82kgai/ha, 1.88kgai/ha, 0.94kgai/ha and 0.00kgai/ha. The no-regrowth plots had a significantly higher shoot density as compared to other stages of regrowth where similarity in lower density of spear grass was obtained at 2 - 4 weeks regrowth plot. The shoot density of spear grass increased significantly as rate of glyphosate decreased where 3.76kgai/ha and 2.82kgai/ha were similar, 2.82kgai/ha and 1.88kgai/ha were also similar while no application had a significantly higher shoot density. Glyphosate applied at the rate of 1.88kgai/ha to 3.76kgai/ha at 2 – 4 weeks of spear grass foliage regrowth effectively control the weed.