Chapter 6 – Microbial Growth
Physical
Requirements
Chemical
Requirements
Growth of
Bacterial
Cultures
Culture
Media
Measurement
of Growth
$100
$100
$100
$100
$100
$200
$200
$200
$200
$200
$300
$300
$300
$300
$300
$400
$400
$400
$400
$400
$500
$500
$500
$500
$500
FINAL ROUND
Topic 1: Physical Requirements
$100 Question
The optimum pH for most bacteria is
a. 1
b. 5
c. 7
d. 9
ANSWER
BACK TO GAME
Topic 1: Physical Requirements
$100 Answer
The optimum pH for most bacteria is
a. 1
b. 5
c. 7 (correct answer)
d. 9
BACK TO GAME
Topic 1: Physical Requirements
$200 Question
A psychrophile grows best at
a. 37°C
b. 0°C
c. 25°C
d. 15°C
ANSWER
BACK TO GAME
Topic 1: Physical Requirements
$200 Answer
A psychrophile grows best at
a. 37°C
b. 0°C (correct answer)
c. 25°C
d. 15°C
BACK TO GAME
Topic 1: Physical Requirements
$300 Question
Organisms that require high salt
concentrations for growth are called
a. Halophiles
b. Extreme halophiles
c. Facultative halophiles
d. Microhalophiles
ANSWER
BACK TO GAME
Topic 1: Physical Requirements
$300 Answer
Organisms that require high salt
concentrations for growth are called
a. Halophiles
b. Extreme halophiles (correct
answer)
c. Facultative halophiles
d. Microhalophiles
BACK TO GAME
Topic 1: Physical Requirements
$400 Question
The energy source utilized by producers in
the hydrothermal vents on the ocean floor
is
a. Light
b. Sulfur
c. Organic molecules
d. Carbon dioxide
ANSWER
BACK TO GAME
Topic 1: Physical Requirements
$400 Answer
The energy source utilized by producers in
the hydrothermal vents on the ocean floor
is
a. Light
b. Sulfur (correct answer)
c. Organic molecules
d. Carbon dioxide
BACK TO GAME
Topic 1: Physical Requirements
$500 Question
In food preservation, salts and sugars are
used because they create a
a. Lower pH
b. Hypertonic environment
c. Hypotonic environment
d. Lower osmotic pressure
ANSWER
BACK TO GAME
Topic 1: Physical Requirements
$500 Answer
In food preservation, salts and sugars are
used because they create a
a. Lower pH
b. Hypertonic environment (correct
answer)
c. Hypotonic environment
d. Lower osmotic pressure
BACK TO GAME
Topic 2: Chemical Requirements
$100 Question
Chemoautotrophs and photoautotrophs
derive their carbon from
a. Carbon dioxide
b. Proteins
c. Lipids
d. Carbohydrates
ANSWER
BACK TO GAME
Topic 2: Chemical Requirements
$100 Answer
Chemoautotrophs and photoautotrophs
derive their carbon from
a. Carbon dioxide (correct answer)
b. Proteins
c. Lipids
d. Carbohydrates
BACK TO GAME
Topic 2: Chemical Requirements
$200 Question
Some microorganisms use gaseous
nitrogen directly from the atmosphere in a
process called
a. Chemosynthesis
b. Photosynthesis
c. Nitrogen fixation
d. Nitrogen synthesis
ANSWER
BACK TO GAME
Topic 2: Chemical Requirements
$200 Answer
Some microorganisms use gaseous
nitrogen directly from the atmosphere in a
process called
a. Chemosynthesis
b. Photosynthesis
c. Nitrogen fixation (correct answer)
d. Nitrogen synthesis
BACK TO GAME
Topic 2: Chemical Requirements
$300 Question
An organism that uses oxygen but can
grow without oxygen is called a(n)
a. Obligate aerobe
b. Facultative aerobe
c. Aerobe
d. Aerotolerant anaerobe
ANSWER
BACK TO GAME
Topic 2: Chemical Requirements
$300 Answer
An organism that uses oxygen but can
grow without oxygen is called a(n)
a. Obligate aerobe
b. Facultative aerobe (correct answer)
c. Aerobe
d. Aerotolerant anaerobe
BACK TO GAME
Topic 2: Chemical Requirements
$400 Question
This organism is killed by atmospheric
oxygen:
a. Aerobe
b. Obligate aerobe
c. Obligate anaerobe
d. Aerotolerant anaerobe
ANSWER
BACK TO GAME
Topic 2: Chemical Requirements
$400 Answer
This organism is killed by atmospheric
oxygen:
a. Aerobe
b. Obligate aerobe
c. Obligate anaerobe (correct answer)
d. Aerotolerant anaerobe
BACK TO GAME
Topic 2: Chemical Requirements
$500 Question
Biofilms reside in a matrix made up
primarily of
a. Lipids
b. Hydrogel
c. Proteins
d. Polysaccharides
ANSWER
BACK TO GAME
Topic 2: Chemical Requirements
$500 Answer
Biofilms reside in a matrix made up
primarily of
a. Lipids
b. Hydrogel
c. Proteins
d. Polysaccharides (correct answer)
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$100 Question
Generation time can best be defined as
a. The length of time it takes for lag
phase
b. The duration of log phase
c. The length of time it takes for a cell
to divide
d. The minimum rate of doubling
ANSWER
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$100 Answer
Generation time can best be defined as
a. The length of time it takes for lag
phase
b. The duration of log phase
c. The length of time it takes for a cell
to divide (correct answer)
d. The minimum rate of doubling
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$200 Question
To cultivate anaerobic bacteria, one must
use special media called
a. Reducing media
b. Oxidizing media
c. Differential media
d. Selective media
ANSWER
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$200 Answer
To cultivate anaerobic bacteria, one must
use special media called
a. Reducing media (correct answer)
b. Oxidizing media
c. Differential media
d. Selective media
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$300 Question
Capnophiles must be grown in high
concentrations of
a. Oxygen
b. Salt
c. Glucose
d. Carbon dioxide
ANSWER
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$300 Answer
Capnophiles must be grown in high
concentrations of
a. Oxygen
b. Salt
c. Glucose
d. Carbon dioxide (correct answer)
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$400 Question
The culture medium that is designed to
increase numbers of desired microbes to
detectable levels is called
a. Complex
b. Selective
c. Enrichment
d. Differential
ANSWER
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$400 Answer
The culture medium that is designed to
increase numbers of desired microbes to
detectable levels is called
a. Complex
b. Selective
c. Enrichment (correct answer)
d. Differential
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$500 Question
The culture medium that is designed to
suppress unwanted microbes while
encouraging desired microbes is called
a. Complex
b. Selective
c. Reducing
d. Differential
ANSWER
BACK TO GAME
Topic 3: Growth of Bacterial Cultures
$500 Answer
The culture medium that is designed to
suppress unwanted microbes while
encouraging desired microbes is called
a. Complex
b. Selective (correct answer)
c. Reducing
d. Differential
BACK TO GAME
Topic 4: Culture Media
$100 Question
To neutralize acids as they are produced
in a culture, what should be added to the
culture medium?
a. Buffers
b. Salts
c. Sugars
d. PABA
ANSWER
BACK TO GAME
Topic 4: Culture Media
$100 Answer
To neutralize acids as they are produced
in a culture, what should be added to the
culture medium?
a. Buffers (correct answer)
b. Salts
c. Sugars
d. PABA
BACK TO GAME
Topic 4: Culture Media
$200 Question
The microbes that grow and multiply in or on
a culture medium are referred to as a(n)
a. Culture
b. Inoculum
c. Specimen
d. Medium
ANSWER
BACK TO GAME
Topic 4: Culture Media
$200 Answer
The microbes that grow and multiply in or on
a culture medium are referred to as a(n)
a. Culture (correct answer)
b. Inoculum
c. Specimen
d. Medium
BACK TO GAME
Topic 4: Culture Media
$300 Question
What can be added to a medium when it is
desirable to grow bacteria on a solid
medium?
a. Glucose
b. Magnesium sulfate
c. Potassium phosphate
d. Agar
ANSWER
BACK TO GAME
Topic 4: Culture Media
$300 Answer
What can be added to a medium when it is
desirable to grow bacteria on a solid
medium?
a. Glucose
b. Magnesium sulfate
c. Potassium phosphate
d. Agar (correct answer)
BACK TO GAME
Topic 4: Culture Media
$400 Question
A culture medium consisting of agar,
human blood, and beef heart is a
a. Complex medium
b. Selective medium
c. Differential medium
d. Chemically defined medium
ANSWER
BACK TO GAME
Topic 4: Culture Media
$400 Answer
A culture medium consisting of agar,
human blood, and beef heart is a
a. Complex medium (correct answer)
b. Selective medium
c. Differential medium
d. Chemically defined medium
BACK TO GAME
Topic 4: Culture Media
$500 Question
The source of nutrients in nutrient agar is
a. Agar
b. Peptone and sodium chloride
c. Peptone and beef extract
d. Nutrient
ANSWER
BACK TO GAME
Topic 4: Culture Media
$500 Answer
The source of nutrients in nutrient agar is
a. Agar
b. Peptone and sodium chloride
c. Peptone and beef extract (correct
answer)
d. Nutrient
BACK TO GAME
Topic 5: Measurement of Growth
$100 Question
A spectrophotometer can be used to
measure
a. Metabolic activity
b. Turbidity
c. Dry weight
d. Pathogenicity
ANSWER
BACK TO GAME
Topic 5: Measurement of Growth
$100 Answer
A spectrophotometer can be used to
measure
a. Metabolic activity
b. Turbidity (correct answer)
c. Dry weight
d. Pathogenicity
BACK TO GAME
Topic 5: Measurement of Growth
$200 Question
Because of time considerations, which
method is usually used to count the
number of bacteria in milk?
a. The direct microscope count
method
b. The pour plate method
c. The spread plate method
d. The most probable number method
ANSWER
BACK TO GAME
Topic 5: Measurement of Growth
$200 Answer
Because of time considerations, which
method is usually used to count the
number of bacteria in milk?
a. The direct microscope count
method (correct answer)
b. The pour plate method
c. The spread plate method
d. The most probable number method
BACK TO GAME
Topic 5: Measurement of Growth
$300 Question
Which method is utilized to ensure that
plates containing 25 to 250 colonies are
counted?
a. The most probable number method
b. Serial dilution
c. Filtration
d. Spread plate method
ANSWER
BACK TO GAME
Topic 5: Measurement of Growth
$300 Answer
Which method is utilized to ensure that
plates containing 25 to 250 colonies are
counted?
a. The most probable number method
b. Serial dilution (correct answer)
c. Filtration
d. Spread plate method
BACK TO GAME
Topic 5: Measurement of Growth
$400 Question
Microorganisms are particularly sensitive
to adverse conditions during the
a. Lag phase
b. Death phase
c. Stationary phase
d. Log phase
ANSWER
BACK TO GAME
Topic 5: Measurement of Growth
$400 Answer
Microorganisms are particularly sensitive
to adverse conditions during the
a. Lag phase
b. Death phase
c. Stationary phase
d. Log phase (correct answer)
BACK TO GAME
Topic 5: Measurement of Growth
$500 Question
Which of the following metabolic activities
can be used to measure microbial growth?
a. Carbon dioxide consumption
b. Oxygen consumption
c. Glucose consumption
d. Salt consumption
ANSWER
BACK TO GAME
Topic 5: Measurement of Growth
$500 Answer
Which of the following metabolic activities
can be used to measure microbial growth?
a. Carbon dioxide consumption
b. Oxygen consumption
c. Glucose consumption (correct
answer)
d. Salt consumption
BACK TO GAME
FINAL ROUND Question
An experiment began with 5 cells and
ended with 160 cells. How many
generations did the cells go through?
a. 20
b. 6
c. 4
d. 5
ANSWER
BACK TO GAME
FINAL ROUND Answer
An experiment began with 5 cells and
ended with 160 cells. How many
generations did the cells go through?
a. 20
b. 6
c. 4
d. 5 (correct answer)
BACK TO GAME