LECTURE AND LABORATORY SYLLABUS Biology and Microbiology 233: Microbial Survey Spring 2010 MWF 11:30 am -12:30 pm Clow 103 Instructor: Dr. Sabrina Mueller-Spitz Halsey Science 151 Phone: 424-1104 email: muellesr@uwosh.edu Office Hours: Wednesday 12:40-2:40 and Thursdays 12-1. Other times are available by appointment. Any time by email. Required text: Microbiology [a human perspective] sixth edition by Eugene W. Nester, Denise G. Anderson, C. Evans Roberts, Jr. and Martha T. Nester. ISBN 978-0-07-299543-5. BIO 233: This is a 4-credit class. It contains a 3-hour lecture component and a 2-hour laboratory component a week. You cannot pass this course if you do not attend and participate in the laboratory component of the class. EMAIL COMMUNICATION and D2L will be used frequently throughout the semester to communicate between Instructors and Students. Emails constitute legal, official University communication. The professor will ONLY respond to polite and detailed emails. Important documents and web links for the laboratory or lecture portion of the course will be posted to D2L (lecture component on D2L) for your convenience. Not checking your email or reading the news item on the course D2L page is not an excuse for performance problems in the class. Contact Academic Computing for assistance with email and D2L. Course Objectives: By the conclusion of this course, you should be able to: 1) Be able to explain the relationship between humans and microbial. 2) To understand immense diversity exhibited within microorganisms in terms of diversity, structure, and metabolic processes. 3) Detail common structural and functional aspects of microorganisms. 4) Explain how microorganisms grow and how unwanted growth can be controlled. 5) To understand the role of microbial genetics in grasping such concepts as pathogenicity, antibiotic resistance and the development of vaccines. 6) Explain how diseases of microbial etiology are spread. 7) Compare the abundance of pathogenic microorganisms to non-pathogenic microorganisms. 8) To gain an understanding of how the human body and various microorganisms interact in terms of health and disease (e.g. defense mechanisms, microbial pathogenicity). 1 Assessment: 1. There will be four (4) 100 point examinations. Each exam will be a multiple choice format. Questions will be taken from material presented in lecture. The final exam will NOT cumulative, however the material of this is class comprehensive, which will be reflected in the exams. Missed exams may not be made up. If you miss an exam due to circumstances beyond your control, you may replace the missed exam by taking a comprehensive exam during the last week of the semester (Requires documentation: doctors excuse, death notice, etc.). 2. Bring quiz cards to EVERY class meeting and take online quizzes. Periodically, quizzes will be given. The instructor will pose a question (or two or three or four. . .) to the entire class. Students will provide their answer by holding up one of four quiz cards (containing the letter A, B, C, or D) that corresponds to the correct answer. Quiz cards can be obtained through D2L. There will be five (5) online quizzes given throughout the semester. The QUIZES WILL BE MENTIONED IN CLASS ONLY! You will have until Midnight on that day to answer the question. Thus, you must be in class every day of the semester to ensure that: 1) you know when an online quiz will be given and 2) what material that online quiz will cover. You may only take online quizzes if you were in class on the day the quiz was announced. You must take the quiz on your own, without the assistance of anyone or any materials. Any violation of this policy will result in disciplinary action as described in relevant University policies. Breaches of academic integrity can result in failing the course and expulsion from the University. 3. Class Participation activities will occur throughout the semester. Some of these activities WILL be collected and scored. These will not be announced before hand, so come to class to make sure you obtain these in-class points. Course Point Breakdown: Assignments Exams (4 @ 100 points each) Class Participation On-line quizzes (5 @ 10 points each) Lab (point breakdown described in lab portion of the syllabus) Point Total Determination of Course Grade: % Grade Points per Letter Grade Unit (cr.) A 92.0-100 4.00 A90.0-91.9 3.67 B+ 88-89.9 3.33 B 82.0-87.9 3.00 B80.0-81.9 2.67 C+ 78.0-79.9 2.33 C 72.0-77.9 2.00 C70.0-71.9 1.67 Value (Points) 400 20 50 430 900 ** The instructor reserves the right to curve grades. If a student does not do well on the first exam, but if there is IMPROVEMENT ON EVERY exam after the 1st one, this will be taken into consideration when final grades are calculated. 2 D+ D DF (Failure) 68.0-69.9 62.0-67.9 60.0-61.9 <60.0 1.33 1.00 0.67 0.00 POLICIES Attendance Policy: Due to the nature of the course, it is imperative that students attend. Information will be provided which relates to the content of the course and related assignments that will be critical to student performance on the course objectives. As described above, missed exams may not be made up. Instead, a comprehensive exam may be taken to replace the missed exam, which will be given the last week of the semester. If you miss a class period, it is your responsibility to obtain notes from a fellow student. Punctuality is encouraged; if late arrival is unavoidable, the student should enter the class in a manner that creates as little disruption as possible. Academic Integrity: Academic dishonesty in any form will not be tolerated. Cheating on an exam, plagiarizing, or any other form of academic dishonesty will be dealt with in accordance with the current UWO Student Discipline Code. Any violation of related to Student Code of Conduct will be dealt with on an individual basis according to the severity of the misconduct. Any questions please consult http://www.uwosh.edu/dean/conduct.htm Special Needs and Accommodations: Students needing special accommodations or special services should inform the Instructor by providing a copy of their Accommodation Recommendation (AR) at the least one week before the exam. Study Effectively and Intelligently. Don’t wait to study until the night before an exam! Allow for enough time to adequately review the material and apply the concepts from the course. Focus your studying to understand the concepts rather than simply memorizing everything. This type of studying will not only help you succeed in this course, but will help you when you are reviewing for nursing boards. The greatest hurtle for any student in a microbiology course is the new vocabulary/names of microbes that must be learned. Repetition remains one of the more effective means of memorizing and understanding information, but it is important that you remember two key points. First, start early in the semester and study regularly. If you review continually and on a regular basis, then you will retain more information and improve your understanding. Second, ask for help if there is something you are struggling with. Remember the key steps of repeated review and short study intervals. Common courtesy: Please turn off all cell phones, ipods, pdas, other bluetooth devices and pagers prior to class. Please remove ear buds. 3 Tentative Lecture Schedule Instructor Reserves the right to change order of topics. Speed of the class maybe faster or slower than listed below depending on questions from the class. However, EXAM dates WILL NOT CHANGE! Week 1 Class Dates Feb 1/3/5 2 Feb 8/10/12 3 Feb 15/17/19 4 Feb 22/24/26 5 March 1/3/5 6 March 8/10/12 March 15/17/19 7 8 9 10 11 12 13 14 March 29/31 April 2 April 5/7/9 April 12/14/16 April 19/21/23 April 26/28/30 May 3/5/7 Topic (s) INTRODUCTION TO MIRCOBIOLOGY HUMANS & MICROBIAL WORLD MOLECULES OF LIFE MICROSCOPY PROKARYOTIC CELLS PROKARYOTIC CELLS STRUCTURE, GROWTH & CONTROL EXAM 1 --FEBRUARY 22nd METABOLISM & PHYSIOLOGY GENETICS PROKARYOTIC CLASSIFICATION PROKARYOTIC DIVERSITY MICROBIAL ECOLOGY MICROBIAL ECOLOGY HUMAN MICROBIOME EXAM 2-- MARCH 19th SPRING BREAK (NO CLASSES) HUMAN MICROBIOME HOST/MICROBE INTERACTIONS IMMUNE RESPONSE VIRUSES & PRIONS ANTIMICROBIALS EXAM 3-- APRIL 19th EPIDEMIOLOGY PATHOGENICITY INFECTIOUS DISEASE INFECTIOUS DISEASE May 10/12/14 INFECTIOUS DISEASE EXAM 4 --MAY 14th Relevant Readings CH 1 CH 2 CH 3 CH 3 & CH 4 & CH 5 CH 6 CH 8 CH 10 CH 11 CH 30 CH 30 Supplemental Material Supplemental Material CH 17 CH 15-16 CH 14 CH 21 CH 20 CH 22, CH 23, CH 24, CH 25 Tentative review sessions for each exam: Feb 19th, March 17th, April 16th, and May 12th. Time and location to be determined. 4 BIO 233: Spring 2010 Microbial Survey Laboratory Syllabus Location: Halsey 174 Instructor:_________________________________________________________ Office:____________________________________________________________ Phone:____________________________________________________________ E-Mail:____________________________________________________________ Office Hours:_______________________________________________________ BIO 233 Laboratory Instructor Information Section Weekday Time 001 Monday 9:10-11:10 a.m. 002 Monday 1:50-3:50 p.m. 003 Wednesday 9:10-11:10 a.m. 004 Thursday 9:40-11:40 a.m. 005 Thursday 1:20-3:20 p.m. 006 Friday 9:10-11:10 a.m. Instructor Dr. Teri Shors Dr. Teri Shors Dr. Teri Shors Dr. Teri Shors Dr. Sabrina Mueller-Spitz Dr. Sabrina Mueller-Spitz Items Needed for Class 1. YOU WILL NEED TO PURCHASE THE FOLLOWING: Required NEW Lab Guide from the UW-Oshkosh Bookstore BIO-233 Microbial Survey Laboratory Manual UW-Oshkosh McGraw Hill Publishing (Do NOT purchase used lab manuals—we require pages to be removed from these manuals. Therefore, used manuals will be missing pages and not useable.) 2. Laboratory Chalk Board Notes are available without cost through the internet package, Desire to Learn. Log on to D2L via the web URL below. These notes will be available at the BIO 233 lecture site under the category "Course Content." It will be found as a topic listed under BIO 233 laboratory documents. http://www.uwosh.edu/d2l (the server will redirect you to the correct secure page) Optional items: Supply “Baggie” from UW-Oshkosh Bookstore 1/pr (called the “Biology kit”) (contains microscope slides and other supplies) Extra Fine Permanent marker Other Laboratory Class Information: 1. Late Lab Reports will not be accepted. There are no make-up quizzes. 2. Quizzes: At the beginning of each laboratory period (with the exception of the first week), there will be short, timed (no more than 10 minutes will be allotted to do this), in-class, quizzes. Focus your studying on reading the chalk talk notes and corresponding pages referred to in the laboratory manual concerning the laboratory work for that day or work being completed from the prior laboratory. Being prepared for the laboratory is the only 5 way to do well in this class. Quizzes cannot be made up. Quizzes may be cumulative--they may ask relevant questions from any previous laboratory. 3. Attendance: You are expected to attend every lab period and to participate fully in each laboratory experiment. Students unable to attend lab must notify the lab instructor prior to the laboratory (phone message to instructor’s office or Biology Dept. Office, E-mail). Labs CANNOT be made up, due to logistical problems. “Section jumping” will not be allowed. 4. Lab Reports and Final Project: This laboratory will be using the scientific method or hypothesis approach. We will expect that students are proficient in the modest use of statistics and graphical presentation of data. Certain laboratory reports and the final project will require this skill in order to do well in the class. For tips on creating bar graphs, printing powerpoint presentations, and lab reports tips, download and read documents available at Desire To Learn: http://www.uwosh.edu/d2l (the server will redirect you to the correct secure page) 5. Grading: The laboratory portion of this course is worth 47% of your BIO 233 grade. The point breakdown for quizzes, lab reports, a final project and exam is listed in the table below. 233 Laboratory : Point Breakdown: Week Assignment Due Activity Quizzes In class quiz (Weeks 2-10) 10 pts. each Week 6 Clinical Sample Unknown: API 20E Week 8 Antibiotic Sensitivity of a Microbial Skin Isolate Week 9 Bread, mold, and environment Excel graph Week 10 Pet Microbe Project Week 11 Lab Report: Microbial Diversity Week 14 Group Project Poster/Presentation Final Exam Held in lab during Week 13. TOTAL LAB POINTS............................................................................................................ .................. Total Points 90 pts. 5 pts. 5 pts. 15 pts. 100 pts. 15 pts. 100 pts. 100 pts. 430 pts. 6. Academic Dishonesty Policy: Cheating on an exam, plagiarizing, or any other form of academic dishonesty will be dealt with in accordance with the current UWO Student Discipline Code. The instructor reserves the right to assign a grade of "F" for the course should circumstances warrant. 7. Common courtesy: Please turn off all cell phones, ipods, pdas, other bluetooth devices and pagers prior to class. 8. 100 Point Final lab exam: This will be a comprehensive written (e.g. short answer/short essay) exam. More information will be provided later in the semester. 6 Laboratory Schedule: Microbial Survey BIO 233 Spring 2010 Week 1 Date February 1-5 2 February 8-12 3 February 15-19 4 February 22-26 5 March 1-5 6 March 8-12 7 March 15-19 8 Mar. 29-April 2 9 April 5-9 10 April 12-16 11 12 13 April 19-23 April 26-30 May 3-7 14 May 10-14 Title of Experiment Orientation to the Microbiology Laboratory Go over SAMPLE QUIZ 1 The Microscope Simple Stains IN CLASS: QUIZ 2 Handling and Examining Cultures Gram Stain Pet Microbes (this on an ongoing lab and will continue through week 9) IN CLASS: QUIZ 3 Pure Culture Techniques Diagnostic Microbiology in Action Respiratory Microorganisms Urine Culture Techniques IN CLASS: QUIZ 4 Use of Enzyme-linked immunoassay (ELISA) Test for Group A Streptococcus Identification Antibiotic sensitivity of Staphylococcus sp. IN CLASS: QUIZ 5 Enterobacteriaceae Identification: The API 20 E System IN CLASS: QUIZ 6 Killing Microbes IN CLASS: QUIZ 7 Synthetic Epidemic Bread, mold, and environment IN CLASS: QUIZ 8 Koch’s Postulates Film: Cryptosporidiosis (Medical Detectives) SPRING BREAK IN CLASS: QUIZ 9 Bacterial Diversity IN CLASS: QUIZ 10 Parasitology: Protozoa and Helminths Fungi: Yeasts and Molds Student Projects PROJECT APPROVAL Student Projects EXPERIMENTS Final Written EXAM (75 minutes, in class) Student Projects RESULTS/DATA COLLECTION Student Presentations : POSTER SESSIONS 7