BIO 233
Microbial Survey Laboratory
SPRING 2006
Instructor:_________________________________________________________
Office:____________________________________________________________
Phone:____________________________________________________________
E-Mail:____________________________________________________________
Office Hours:_______________________________________________________
1.
YOU WILL NEED TO PURCHASE THE FOLLOWING:
Required Lab Guide from the UW-Oshkosh Bookstore
BIO-233 Microbial Survey Laboratory Manual
UW-Oshkosh
McGraw Hill Publishing
Supply “Baggie” from UW-Oshkosh Bookstore 1/pr (called the “Biology kit”)
2.
Laboratory Chalk Board Notes are available through D2L:
3.
Lab Reports and Final Project: This laboratory will be using the scientific method
or hypothesis approach. We will expect that students are proficient in the modest use of
statistics and graphical presentation of data. Certain laboratory reports and the final
project will require this skill in order to do well in the class. For tips on creating bar
graphs, printing powerpoint presentations, and lab reports tips, visit D2L:
4.
Grading: The laboratory portion of this course is worth 40% of your BIO 233
grade. The point breakdown for quizzes, lab reports, a final project and exam is
listed in the table below.
Specific Date or Week Due
Activity
Total
Points
Quizzes
In class quiz (Weeks 2-10)
90 Pts.
10 pts. each
Due the following lab period for each case of Case Studies (10 total) from the Lab Manual @
10 pts.
that week
1 pt./week
Week 5
Clinical Sample Unknown: API 20E
10 pts.
Week 7
Antibiotic sensitivity of a microbial skin isolate
5 pts.
Week 9
Lab Report 1 : Bread, mold, and environment
15 pts.
Week 10
Pet Microbe Project
100 pts.
Week 11
Lab Report 3: Microbial Diversity
15 pts.
Week 12
Lab Report 2: Koch’s Postulates
15 pts.
Week 14
Group Project Poster/Presentation
100 pts.
Final Exam
50 pts.
Held in lab during Week 13.
TOTAL LAB POINTS.......................................................................................................................................410 pts.
5.
Quizzes: At the beginning of each laboratory period (with the exception of the
first week), there will be short, timed (no more than 5 minutes will be allotted to
do this), in-class, quizzes based on reading the chalk talk notes and pages
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referred to in the laboratory manual via the chalk talk notes concerning the
laboratory work for that day or work being completed from the prior
laboratory. Being prepared for the laboratory is the only way to do well in
this class. Quizzes cannot be made up.
6.
Late lab reports and case studies will not be accepted. There are no make-up
quizzes.
7.
Academic Dishonesty Policy: Cheating on an exam, plagiarizing, or any other
form of academic dishonesty will be dealt with in accordance with the current
UWO Student Discipline Code. The instructor reserves the right to assign a grade
of "F" for the course should circumstances warrant.
8.
Attendance: You are expected to attend every lecture period and to participate
fully in each laboratory experiment. Students unable to attend lab must notify the
lab instructor prior to the laboratory (phone message to instructor’s office or
Labs CANNOT be made up,
due to logistical problems. “Section jumping” will
not be allowed.
Biology Dept. Office, E-Mail).
9.
Common courtesy: Please turn off all cell phones and pagers prior to class.
10.
Answers to Cases and Quizzes: Will be posted the week after all students in
each lab section have turned them in.
11.
Final lab exam: This will be a comprehensive written (short answer/short
essay) exam. More information will be provided later in the semester.
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Laboratory Schedule: Microbial Survey BIO 233
Week
1
Date
Jan. 3 0– Feb.
3
2
Feb. 6 – 10
3
Feb. 13-17
4
Feb. 20-24
5
Feb. 27 –
Mar. 3
6
Mar. 6 – 10
7
Mar. 20 – 24
8
Mar. 27 –
Mar. 31
9
Apr. 3 – 7
10
Apr. 10 – 14
11
12
13
Apr. 17 – 21
Apr. 24 – 28
May 1 – 5
14
May 8 – 12
Title of Experiment
Orientation to the Microbiology Laboratory
Go over SAMPLE QUIZ 1
The Microscope
Simple Stains
IN CLASS: QUIZ 2
Handling and Examining Cultures
CASE 1 DUE
Gram Stain
Pet Microbes (this on an ongoing lab and will continue through week 10)
IN CLASS: QUIZ 3
Pure Culture Techniques
CASE 2 DUE
Diagnostic Microbiology in Action
Respiratory Microorganisms
Urine Culture Techniques
IN CLASS: QUIZ 4
Enterobacteriaceae Identification: The API 20 E System
Isolation of Staphylococcus sp. from Human Skin
CASE 3 DUE
IN CLASS: QUIZ 5
Use of Enzyme-linked immunoassay (ELISA)Test for Group A
Streptococcus Identification
HIV diagnosis
CASE 4 DUE
IN CLASS: QUIZ 6
Synthetic Epidemic
Killing Microbes
CASE 5 DUE
IN CLASS: QUIZ 7
Bread, mold, and environment
CASE 6 DUE;
Skin Isolate Table Due
IN CLASS: QUIZ 8
Koch’s Postulates
Film: Cryptosporidosis (Medical Detectives)
CASE 7 DUE
IN CLASS: QUIZ 9
Microbial Diversity
CASE 8 DUE
IN CLASS: QUIZ 10
Parasitology: Protozoa and Helminths
CASE 9 DUE
Fungi: Yeasts and Molds
Student Projects
PROJECT APPROVAL/CASE 10 DUE
Student Projects
EXPERIMENTS
Final Written EXAM (1 hour, in class)
Student Projects
Student Presentations :
RESULTS/DATA COLLECTION
POSTER SESSIONS
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