NUS Institutional Animal Care and Use Committee 000/16 Date revised: Jan 2016 Form expiry date: 30 Jun 2016 Appendix 2 Changes in the number of animals 1. Choose the relevant table to complete: Replacing approved species /strains / type S/N 1 2 3 Species Strains Pain Type Cat. (♂, ♀, preg) Age or Weight Number of Animals Pain Type Cat. (♂, ♀, preg) Age or Weight Number of Animals Pain Type Cat. (♂, ♀, preg) Age or Weight Number of Animals New species Species to replace New species Species to replace New species Species to replace Adding new species /strains / type S/N Species Strains 1 2 3 Increase in the number of approved species /strains /type S/N Species Strains 1 Approved Additional 2 Approved Additional 3 Approved Additional 2. Justify the need to increase the number: Replacing approved species / strains / type. Please complete Appendix 1. Adding new species /strains / type to the protocol. Please complete Appendix 1. As described below: 3. Number of animals required. (a) Indicate which of the following is required for the additional animal: Additional treatment groups of the approved experimental design. Amendment 1 Appendix 2 NUS Institutional Animal Care and Use Committee 000/16 Repeat of an approved experiment. New experiment. Others. Please describe: (b) Show how the number of animals required is derived: 4. Justification of sample size: Same sample size as justified in the approved protocol. Sample size different from the approved protocol, as justified below: 5. Are you using a new procedure? Yes. Please complete Appendix 3. No. Indicate which approved procedure will be used on the additional animals -- End of Appendix 2 -- Amendment 2 Appendix 2