LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2011
BT 2817 - RECOMBINANT DNA & GENE CLONING
Date : 2/4/2011
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART – A
ANSWER ALL THE QUESTIONS
1. Choose the correct answer:
(5 x 1 = 5 marks)
1) Natural competence is found in which organism?
a) Bacillus subtilis b) Agrobacterium c) E.coli d) Rhizobium sp
2) Replicative form of DNA is found in
a) pUC 18 b) M13 c) pSC101 d) EMBL3
3) YEp copy number per cell is
a) 1-20 b) 25-200
c) 1-2
d) 10-15
4) Northern hybridization is between
a) protein & protein b) protein & DNA
c) DNA & DNA d) RNA & DNA
5) Sir Alec Jeffrey is associated with
a) PCR b) DNA Fingerprinting c) Endonucleases d) Ligases
II. State True or False, if false give reason
(5 x 1 = 5 marks)
6) Bal 3I is used for gene cloning.
7) EMBL3 is a replacement vector.
8) SV40 can be used as a vector.
9) Carbanicillin is used for bacterial transformation.
10) The optimum temperature for Taq polymerase is 45 oC.
III. Complete the following:
(5 x 1 = 5 marks)
11) __________ is the first recombinant plasmid vector.
12) YAC stands for ________
13) Late genes are found in __________ vector.
14) cDNA mapping is done by ________________.
15) RFLP stands for _________
IV. Answer the following, each within 50 words only
16) Define cassettes
17) Mention the various kinds of ligases and its origin?
18) Comment on binary vector.
19) Define South- Western hybridization.
20) How do you perform virtual PCR?
PART - B
(5 x 1 = 5 marks)
V. Answer the following each within 350 words only, draw diagrams where required
(8 x 5 = 40 marks)
21) a) Differentiate between linkers and adaptors
OR
b) Distinguish between scorable and selectable markers and give two examples of each
22) a) Compare and contrast transformation and cloning in E.coli and Bacillus subtilis
OR
b) Write an account on pBluescript vector
23) a) Schematically explain the cloning in Baculovirus expression system
OR
b) Differentiate between YEp and YCp vectors
24) a) Give an account on foot-printing
OR
b) Write briefly on in vitro translation
25) a) Explain with suitable illustration the site directed mutagenesis
OR
b) What are all the main areas of consideration for safety aspects in Biotechnology?
PART – C
VI Answer any two of the following, each within 1500 words only
26) Discuss the following
i) Various enzymes that modify the ends of DNA
ii) The desirable properties of vectors and construction of pBR322
(2 x 20 = 40 marks)
(10 marks)
(10 marks)
27) Describe the procedure of heterologous gene expression in Xenopus oocytes
with diagrams
(10 + 10 marks)
28) Describe Southern hybridization schematically
(10+10 marks)
29) Schematically describe how recombinant insulin can be produced
(10 + 10 marks)
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