LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
THIRD SEMESTER – NOVEMBER 2012
BT 3953 - PHARMACEUTICAL BIOTECHNOLOGY
Date : 10/11/2012
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART – A
Answer all the questions
(20 marks)
1. Choose the correct answer:
(5 x 1 = 5 marks)
(1) Which is the first recombinant vaccine produced in India ?
(a) Hepatitis B
(b) Polio
(c) Flu
(d) Smallpox
(2) Which of the following is treated with erythropoietin?
(a) Cancer
(b) Anemia
(c) Diabetes
(3) Which of the following causes bronchodilation?
(a) Prostaglandin (b) Statin
(c) EPO
(d) Hypertension
(d) insulin
(4) Which one of the following is a complement inhibitor?
(a) CD 90 (b) FK506 (c) Tumour necrosis factor (d) Decay accelerating factor
(5) In drug discovery, the term ‘hits’ refers to drugs that
(a) bind specifically to the target
(b) do not bind to the target
(c) are Illegal
(d) bring good profit
II. State whether the following are true or false; if false give reason (5 x 1= 5 marks)
(6) Ayurveda is 5000 yrs old.
(7) Biosensor is used in glucometer.
(8) Bromometabolites are produced by Fungi.
(9) The Herpes Simplex virus thymidine kinase gene can be used as a suicide gene in gene therapy.
(10) Amino acids can be used as excipients to stabilize recombinant protein products.
III. Complete the following:
(5 x 1 = 5 marks)
(11) Oldest traditional medicine known to mankind is ________
(12) Largest fermentor in the world produces ________
(13) ________ is the active component of Ephedra
(14) Of all the viruses that a pig organ can harbor the recent focus is now on ________ virus after the
finding that it can infect human kidney cell line 293.
(15) Affymetrix microarrays are produced by the technique of ________ synthesis on a silica substrate.
IV. Answer the following, each within 50 words only
(16) Define drug.
(5 x 1 = 5marks)
(17)
(18)
(19)
(20)
What is Rituximab?
What are prostaglandins?
In gene therapy for rheumatoid arthritis which was the target gene used?
What is meant by high throughput screening?
PART – B
(5×8 = 40 marks)
Answer the following questions, each in about 500 words only. Draw diagrams wherever necessary.
21 (a) Write the advantages and disadvantages of prokaryotic expression system.
OR
(b) Explain briefly homeopathic system of medicine.
22 (a) Describe the production of monoclonal antibody with diagrammatic representation
OR
(b) How would you classify drug types?
23 (a) Give an account on antibacterial compounds.
OR
(b) Discuss the use of bioconversion for drug production.
24 (a) Write a note on gene therapy for neurological disorders.
OR
(b) Describe the immunological aspects and clinical applications of xenotransplantation.
25 (a) Discuss the steps in rational drug design for unknown targets.
OR
(b) What are designer drugs? Elaborate giving suitable examples.
PART – C
(2×20 = 40 marks)
Answer any TWO of the following questions, each in about 1500 words; Draw diagrams wherever
necessary.
(26) Discuss the following:
(i) Formulation of biotechnology products (15)
(ii) Combinatorial biosynthesis
(5)
(27) Give an account of the different gene delivery strategies and mechanisms used for gene therapy. Add
a note on gene therapy for cardiovascular and infectious diseases.
(28) Explain the history of Biotechnology over the last 50-60 years.
(29) Describe phytopharmaceuticals with examples.