LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – NOVEMBER 2012
BT 1828 - IMMUNOLOGY & IMMUNOTECHNOLOGY
(ONLY FOR 12 BATCH STUDENTS)
Date : 09/11/2012
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART – A
Answer all the questions
1. Choose the correct answer:
(1) Antibody-dependent cellular cytotoxicity is mediated by
(a) eosinophils
(b) natural killer cells
(c) monocytes
(20 marks)
(5 x 1 = 5 marks)
(d) none of the above
(2) Which one of the following hormones suppresses the expression of pro-inflammatory genes?
(a) adrenalin (b) glucocorticoids (c) noradrenalin (d) prolactin
(3) An example of a secondary immunodeficiency is
(a) severe combined immunodeficiency
(b) leucocyte adhesion deficiency
(c) acquired immunodeficiency syndrome
(d) neutropenia
(4) A vaccine prepared by inactivation of the pathogen by formaldehyde is
(a) Sabin polio vaccine
(b) Salk polio vaccine
(c) Bacillus Calmette-Guerin
(d) antitoxin
(5) Antibodies conjugated with gold particles are used in
(a) magnetic cell sorting
(b) immunosensors
(c) western blotting
(d) immunoelectron microscopy
II. State whether the following are true or false; if false give reason:
(5 x 1= 5 marks)
(6) Monocytes are agranulocytes.
(7) An individual can inherit a maximum of 12 different MHC II molecules.
(8) Autoantibodies to intrinsic factor will lead to Goodpasture’s syndrome.
(9) Monoclonal antibodies are antibodies produced by different B-cells for one specific epitope of an
antigen.
(10) In radioimmunoassay, the greater the radioactivity of the test sample, the greater is its concentration.
III. Complete the following:
(5 x 1 = 5 marks)
(11) ________ protein secreted by cytotoxic T cells creates pores in the target cell membrane.
(12) The MHC is present on chromosome ________.
(13) An organ transplant from animals to human is known as a ________.
(14) The swine flu vaccine developed by Pune’s Serum Institute of India is ________ .
(15) A fluorescent label used for immunofluorescence is ________.
IV. Answer the following, each within 50 words only:
(5 x 1 = 5marks)
(16) What are superantigens?
(17) Give any two reasons for the low immune response to Plasmodium falciparum.
(18) Name any two immunosuppressants used for graft rejection.
(19) What is the use of adding aminopterin in the HAT medium used for hybridoma selection?
(20) What is meant by immune panning?
PART – B
Answer the following questions, each in about 500 words only.
Draw diagrams wherever necessary:
(5×8 = 40 marks)
21 (a) Explain the following:
(i) Structure of major histocompatibility molecules.
(ii) Cell surface molecules involved in the activation of T-lymphocytes.
OR
(b) How are lipid antigens processed and presented to T cells?
22 (a) Explain the role of microRNAs in immune regulation.
OR
(b) Describe the immune responses mounted against bacteria and bacterial evasion mechanisms.
23 (a) Write a note on hypersensitivity reactions involved in contact dermatitis.
OR
(b) Explain how the immune system responds to tumours and how tumours evade it.
24 (a) Give an account of the current advances and issues in AIDS vaccine development.
OR
(b) Write a note on the applications of monoclonal antibodies.
25 (a) Describe the technique of immuno-PCR.
OR
(b) Explain how fluorescence activated cell sorting is done.
PART – C
Answer any TWO of the following questions, each in about 1500 words;
Draw diagrams wherever necessary:
(2×20 = 40 marks)
(26) What are the different types of vaccines? Describe the production of a recombinant peptide vaccine
for Foot and Mouth disease virus and an attenuated vaccine for
V. cholerae.
(27) Write notes on:
(i) Mechanism of immunoglobulin by gene rearrangement
(ii) Antibody engineering in E.coli.
(28) Give a detailed account on the different pathways of the complement system.
(29) Explain the types, mechanism of induction and treatment of autoimmunity.
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