LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
FIRST SEMESTER – NOVEMBER 2012
BT 1827 - MICROBIOLOGY
Date : 07/11/2012
Time : 1:00 - 4:00
Dept. No.
Max. : 100 Marks
PART- A (20marks)
Answer all questions
I Choose the correct answer:
(5 x 1 = 5marks)
1. Match A) Electron accelerator B) Hot air oven C) Arnold sterilizer
with i) tyndallization
ii) radiation
iii) filtration
a) A-ii, B-iii, C-iv, D-i
b) A-ii, B-i, C-iii, D-iv
c) A-ii, B-iv, C-iii, D-i
d) A-ii, B-iv, C-i, D-iii
D) Asbestos pad
iv) dry heat
2. Statement A: Glycolysis is the most common pathway of glucose metabolism.
Statement B: It is found only in anaerobes.
a) Statement A is correct but Statement B is incorrect
b) Statement B is correct but Statement A is incorrect
c) Both A and B are correct
d) Both A and B are incorrect
3. In which of the following process, bacteriophages are involved:
a) transduction
b) transformation
c) conjugation
d) translocation
4. ______________ antibiotic inhibits viral replication.
a) actinomycin B b) gentamycin
c) actinomycin D
d) streptomycin
5. The bullet shaped virus is
a) Adenovirus
b) Rhabdovirus
d) Papovavirus
c) Retrovirus
II State whether the following statements are True or False; if false, give reason:
(5 x 1 = 5 marks)
6. Archaebacterial cell wall is mainly made up of peptidoglycan.
7. McConkey agar is a differential medium for LFs and NLFs.
8. Vitamin C is riboflavin and is mainly found in apples.
9. Virions are resistant to heat and UV radiation.
10. IRES distinguishes Orthomyxovirus from other RNA viruses.
III Complete the following:
(5 x 1 = 5marks)
11. Complete ___________ of all forms of microbial life is called ___________.
12. The photosynthesis which does not involve the evolution of _____ is called _____
photosynthesis.
13. The main industrial source of penicillin is __________ _________.
14. When the HIV level increases in blood, the ___________ cells decreases.
15. Viral RNA genomes that are complementary to viral mRNA genome are called __________.
IV Answer the following each within 50 words
(5 x 1 = 5 marks)
16. What is credes method?
17. Distinguish between photoorganotrophs and chemolithotrophs.
18. Define the term specialized transduction.
19. Define Prion.
20. What are the symptoms of cytopathic effect?
PART- B
V Answer the following, each within 500 words; Draw diagrams wherever necessary:
(5 x 8 = 40marks)
21. (a) Give an account of components present external to bacterial cell wall.
OR
(b) Give a comparative account of archaebacteria and eubacteria.
22. (a) Explain the growth curve pattern in bacteria.
OR
(b) Discuss the energy production by anaerobic processes.
23. (a) Describe the process of bacterial transformation.
OR
(b) Expand the following with a footnote each:
i) PG ii) SEM iii) BMDB iv) HEPA v) EMB vi) Hfr vii) DMC viii) ETS
24. (a) Write short notes on
i) Helical virus
ii) Polyhedral virus
OR
(b) Explain how viruses are cultivated using cell culture method.
25. (a) Describe the structure and genome organization of HIV.
OR
(b) Give a brief account on the replication of TMV.
PART - C
VI Answer any TWO of the following, each within 1500 words; Draw diagrams wherever
necessary:
(2 x 20 = 40marks)
26. Give an account of control of microbes using:
a) radiation
b) filtration
c) halogens
d) gaseous chemosterilizers
27. What is a culture medium? Write about the different types of culture media.
28. Explain the different stages involved in the infection of virus host cell with respect to
ssRNA and sense strand virus.
29. Write about
a) control of HIV
b) hemagglutination
c) sandwich ELISA
d) ICTV classification
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