LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2012
BT 2820 - STRUCTURAL BIOLOGY & BIOINFORMATICS
Date : 24-04-2012
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART-A (20marks)
Answer all the questions
I Choose the correct answer:
(5 x 1 = 5 marks)
1. _____________ results from hindered rotation about single bonds.
a) Stereoisomerism
b) Atropisomerism
c) Rotomerism
d) Transisomerism
2. The position in the _____________ repeat are usually labelled as abcdefg.
a) Mono
b) Penta
c) Tetrad
d) Heptad
3. Fusion protein for treating cryopyrin is ____________ .
a) Rilonacept
b) Saponins
c) Dexamethasone
d) Fusidic acid
4. ____________ database uses SAKURA to accept nucleotide sequences.
a) NCBI
b) EMBL
c) DDBJ
d) NDB
5. A statistical technique for determining the change in gene expression is _____________.
a) OPTICS
b) DBSCAN
c) MUSCLE
d) SAM
II State whether the following statements are True or False; if false give reason:
(5 x 1 = 5 marks)
6.Φ controls the backbone atoms C-N-Cα-C’.
7. Collagen5 maintains the integrity of the tissues.
8. Ia is a weak helix former.
9. Grenoble makes use of mouse models for medical related problems.
10. TAS2R38 protein in humans is responsible for sweet taste.
.
III Complete the following:
(5 x 1 = 5 marks)
11. ______________ RNA adds peptide tags protein to degrade incorrect proteins.
12. Mineral content of bones and hard tissues in mammals is _____________.
13. ______________ disease is caused by mutation of prion protein PRNP.
14. WormBase has gene expression data of ______________ genome.
15. ______________ is a soft ionization technique.
IV Answer the following questions, each in about 50 words:
(5 x 1 = 5 marks)
16. Explain Chargaff’s rule.
17. Define sickle cell anaemia.
18. What are chaperons?
19. What is multiple sequence alignment?
20. Define transcriptomics.
PART-B
Answer the following, each in about 500 words; Draw diagrams wherever
Necessary:
(5 x 8 = 40 marks)
21.(a) Explain double helical Watson-Crick model of DNA.
OR
(b) What is Stereochemistry? Explain the concept of conformation in macromolecules.
22. (a) Explain tertiary and quaternary structures of proteins.
OR
(b) Briefly summarise the molecular pathology of haemoglobin C disease.
23. (a) Define protein stability? Explain the factors influencing them.
OR
(b) Give about the importance on protein engineering.
24. (a) Briefly explain PDB and SCOP.
OR
(b) Explain repeat sequences with a suitable diagram.
25. (a) Write notes on two protein identification programs.
OR
(b) Explain on integrating gene expression data for pathway information.
PART-C
Answer any TWO of the following, each in about 1500 words. Draw diagrams wherever
necessary :
(2 x 20 = 40 marks)
26. Give an account on the different structures of RNA and add a note on supercoiling.
27. Explain in brief the occurrence, structure, properties and disorders of collagen.
28. Explain Chou-Fasman scheme of protein structure prediction.
29. Give a detailed description on Human Genome Project.
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