LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIO TECHNOLOGY
SECOND SEMESTER – APRIL 2012
BT 2817 - RECOMBINANT DNA & GENE CLONING
Date : 17-04-2012
Time : 9:00 - 12:00
Dept. No.
Max. : 100 Marks
PART – A
Answer All The Questions:
(20 marks)
I. Choose the correct answer:
(5 x 1 = 5 marks)
1) Competent cells are made using
a) Calcium Chloride b) NaCl c) EDTA d) Calcium hydroxide
2) Replicative form of DNA is found in
a) pUC 18
b) M13 c) pSC101 d) EMBL3
3) YEp copy number per cell is
a) 1-20
b) 25-200
c) 1-2
d) 10-15
4) Western blotting is between
a) protein & protein b) protein & DNA
c) DNA & DNA d) RNA & DNA
5) Sanger is associated with
a) PCR b) DNA sequencing c) Endonucleases d) Ligases
II. State whether the following are True or False, if false give reason
6) Exo III is used for gene cloning.
7) EMBL4 is a replacement vector.
8) YRp is a lambda vector.
9) DNase I is used for gel retardation assay.
10) Magnesium chloride is used in PCR.
III. Complete the following each in about 50 words only
11) __________ is the first recombinant plasmid vector.
12) pBR 322 has ________ marker.
13) Late genes are found in __________ vector.
14) cDNA mapping is done by ________________.
15) RAPD stands for _________ .
IV. Answer the following each in about 50 words only
16) Define selectable markers with an example.
17) What are lambda replacement vectors?
18) Comment on co-integrate vector.
19) Define Northern hybridization.
20) What is virtual PCR?
(5 x 1 = 5 marks)
( 5 x 1 = 5 marks )
( 5 x 1 = 5 marks )
PART - B
Answer any five questions each in about 500 words only; Draw diagrams wherever necessary
(5 x 8 = 40 marks)
21a) Distinguish between linkers and adaptors.
OR
b) Enlist the various modifying enzymes. Give brief notes on endo and exonucleases.
22a) Write a note on lambda ZAP vector.
OR
b) Explain the life cycle of M13 vector.
23a) Discuss the properties of YCp and YEp fungal vectors .
OR
b) Schematically explain the cloning in Baculovirus expression system.
24 a) Give an account on foot-printing.
OR
b) Discuss the screening of library by colony blot hybridization.
25a) What is PCR? List out any five variables of PCR.
OR
b) Explain with suitable illustration the site directed mutagenesis.
PART - C
Answer any TWO of the following, each in about 1500 words only; Draw diagrams wherever
necessary
(2 x 20 = 40 marks)
26) Discuss the following
i) The properties of various types of restriction enzymes.
ii) The cloning in Bacillus subtilis .
27) Describe the procedure of heterologous gene expression in Xenopus Oocytes.
28) Explain the construction of genomic and cDNA libraries.
29) Write an essay on the production of recombinant insulin.
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