LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION – BIOMEDICAL INSTRUMENTATION FIRST SEMESTER – NOV 2006 AG 01 BI 1800 - TOOLS & TECHNIQUES IN CELL BIOLOGY Date & Time : 26-10-2006/1.00-4.00 Dept. No. Max. : 100 Marks SECTION – A Answer all questions : 10 X 2 = 20 1. What is autoanalyser ?Mention its uses . 2. What are the features of cell cycle?. 3. Comment on the genetic material of prokaryotes . 4. Define velocity and buoyant density . 5. What is “fluid mosaicmodel”? 6. What is called microtubular organelles ? 7. What is Autofluorescence ? 8. Define metachromasia ? 9. Compare euromartin with heterochromatin. 10. What is stem cell theropy ? SEC TION– B Answer any 4 questions : 4 X 10 = 40 11. Write an account on HPLC and its biological applications . 12. Explain the special features of FISH technique and give a note on its advantages. 13. Describe the cytochemical characteristics of nucleus in eukaryotes . 14. Describe the structure and types of RNA and add a note on the role of mRNA 15. What are the characteristics of Hybridisation Probes? 16. Give an account of the sygnificance of culture media in animal tissue culture. SEC TION– C Answer any 2 questions: 2 X 20 = 40 1. Write an essay on the characteristics of cancer cells. Comment on the new and developing therepies for treatment of cancer ? 18. Explain PCR and its applications in the identification of genetic diseases . 19. Give an account of physical principles involved phase contrast microscopy and comment on its biological applications . 20. Write an essay on cell fractionation technique and isolation of cellular components. Add a note on ultra centifugation _____________________