LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
M.Sc. DEGREE EXAMINATION - BIOMED.INSTRUMENTATION SC.
SECOND SEMESTER – APRIL 2008
BI 2801 - SEPARATION TECH. OF BIOMOLECULES
Date : 17/04/2008
Time : 1:00 - 4:00
Dept. No.
RS 05
Max. : 100 Marks
SECTION – A
Answer All the Questions
(10 x 2 = 20 Marks)
1. Define ‘Ligands’ and give examples
2. What is molecular sieve?
3. State the significance of two major centrifugation techniques.
4. What is affinity elution?
5. Bring out the significance of HPLC
6. Define the term ‘Retention Time’.
7. Name the types of optical system.
8. Differentiate capillary blotting from electro blotting
9. Explain the term Rf value.
10. Enumerate the application of affinity chromatography
SECTION – B
Answer Any Four Questions
(4 x 10 = 40 Marks)
11. Discuss the principle, operation, and applications of GLC.
12. Explain the principle of Isoelectric Focusing and add its important applications.
13. Discuss the mechanism of gel permeation chromatography and its applications.
14. How will you identify the protein of your interest by using blotting techniques?
15. Discuss the principle, procedure and application of agarose gel electrophoresis.
16. Give the mechanism of ion exchange chromatography.
SECTION – C
Answer Any Two Questions
(2 x 20 = 40 Marks)
17. “Separation of amino acid based on their partition coefficient on the paper
chromatogram” Justify.
18. Describe the principle, procedure and applications SDS-PAGE.
19. Give the principle, methodology, and applications of density gradient centrifugation.
20. Comment on: a) Densitometer b) Immunoelectrophoresis and c) FID
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