SPANISH TYPE CULTURE COLLECTION (CECT) Parc Científic Universitat de València Catedrático Agustín Escardino, 9 46980 Paterna (Valencia) Tel: +34 96 354 4612 www.cect.org ACCESSION FORM FOR SAFE DEPOSIT OF STRAINS Type of microorganism Bacteria Archaea Fungi Yeast To be completed by the CECT CECT accession number: Reception date: Strain designation *Scientific name (or proposed): *Strain designation used by the depositor: Other remarks about the strain: Origin of the strain *Isolated by (person and date): SAFE DEPOSIT *Source of isolation: Information relating to the Convention on Biological Diversity (CBD – http://www.biodiv.org/) *Country of origin and/or geographical location: No organism can be accepted without this information When applicable: Sampling agreement (Prior Informed Consent or PIC) Name and address of the person or organization who issued the PIC Please attach documents Cultivation of the strain Culture media (no. CECT or composition): *Incubation temperature: *Incubation time: *Metabolism: aerobic microaerophilic facultative anaerobe obligate anaerobe *Special requirements (light, gas phases, resistance, etc) Does this microorganism survive freezing? YES Additional data Does the microorganism harbour a natural plasmid? (If so, fill in annex 1A) YES NO Is the microorganism genetically manipulated? (If so, fill in annex 1B) YES NO Fields marked with an asterisk (*) are compulsory Ed_06_20140724 NO RISK ASSESSMENT In agreement to Spanish regulations (RD 664/1997) depositors are requested to provide information about risk assessment of the strain. The CECT accepts strains until risk group 3(*). Risk group: Pathogenic for: □ □ group 1 animals □ □ group 2 □ plants specify: group 3(*) IMPORTANT: 1. The CECT will send the invoice corresponding to the deposit and annual maintenance. Depositor has 3 months to make effective the payment. After this time, the CECT reserves the right to destroy the deposited strain if the original invoice has not been paid. 2. CECT is not responsible for the authenticity and purity of the deposited strain if the confirmation form is not duly completed and returned after three months. SAFE DEPOSIT 3. According to the CBD (Convention on Biological Diversity), is the depositor responsibility to ensure that the samples from which the strains were isolated, were collected according to the legislation of the country of origin and that the deposit of the strains in an open collection does not infringe any national regulation. NOTE: The undersigned certifies that the information provided on this form is true. This strain will not be included in the public catalog of CECT. The distribution of the strain is restricted to third parties and will not be delivered without the permission of the depositor. Billing information Organization’s name: Address: VAT or TAX ID number: Depositor *Name: *Institution: Postal address: *Email: Telephone: Fax: *Date: *Signature: Fields marked with an asterisk (*) are compulsory Ed_06_20140724 ANNEX I A. Fill only if the microorganism harbors a natural plasmid Plasmid designation: Natural host: Molecular weight of plasmid: Plasmid isolated by (references): Is the plasmid self-transmissible? YES NO Genetic properties of the plasmid (R: resistances, etc): Unique restriction sites (please include map or reference): B. Fill only if the microorganism if genetically manipulated Please, attach reprints or complete as far as possible In agreement to Spanish regulations (RD 15/1994) concerning genetic engineering, depositors are requested to provide information about: Data concerning the host organism: SAFE DEPOSIT Risk group: Pathogenic for: □ □ group 1 animals □ □ group 2 □ plants specify: group 3(*) Sensitivities Resistances Auxotrophies Special properties (restriction/modification system, genetic recombination, etc): Data concerning the donor organism: Risk group: Pathogenic for: □ □ group 1 animals □ □ group 2 □ plants specify: group 3(*) Description of the cloned DNA fragment (please, attach reprints or complete as far as possible): Size of the cloned DNA (in bp): □ complete genome □ subgenomic Fields marked with an asterisk (*) are compulsory □ subgenic Ed_06_20140724