Aim: How can we do color
adjustment in ImageJ?
Day 3
Why do color adjustment?
• Most pictures are in
color
• Some details are lost
in photographs
• Easier to analyze in
binary
Getting Started
• Open Picture
• Set Scale
• Use Ellipse or other
appropriate tool to
select area of analysis
• Edit
• Clear outside
Adjusting color
• Type
– 8-bit
• Select grayscale, fill
in glare if necessary
• Image
– Adjust threshold
– Slide lower scale as
needed
– Stop when b/w
matches original
• Analyze
– Analyze particles
– Show outlines
Data Analysis
• Copy and paste data
to Excel
• Foumulas
– Autosum (Ʃ)
at bottom of area
column
Assignment
• All Students:
• Pick Two images (of 1, 2, and 4)
• Send Screen Shots of outlines and Excel Files