Aim: How can we do color adjustment in ImageJ? Day 3 Why do color adjustment? • Most pictures are in color • Some details are lost in photographs • Easier to analyze in binary Getting Started • Open Picture • Set Scale • Use Ellipse or other appropriate tool to select area of analysis • Edit • Clear outside Adjusting color • Type – 8-bit • Select grayscale, fill in glare if necessary • Image – Adjust threshold – Slide lower scale as needed – Stop when b/w matches original • Analyze – Analyze particles – Show outlines Data Analysis • Copy and paste data to Excel • Foumulas – Autosum (Ʃ) at bottom of area column Assignment • All Students: • Pick Two images (of 1, 2, and 4) • Send Screen Shots of outlines and Excel Files