SCIENTIARUM PO LONORUM
ACTA
c Copyright by Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu
Acta Sci. Pol., Technol. Aliment. 11(3) 2012, 249-257
pISSN 1644-0730 eISSN 1889-9594 www.food.actapol.net/
ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS FROM ANNONA SQUAMOSA, AND
CATUNAREGAM NILOTICA METHANOLIC EXTRACT
Abdalbasit Adam Mariod1 , Siddig Ibrahim Abdelwahab2, Sara
Elkheir1,
Yousif Mohamed Ahmed1, Putri Narrima Mohd Fauzi3, Cheah Shiau
Chuen3
Food Science and Technology Department, College of Agricultural Studies, Sudan University of Science
and Technology
P.O. Box 71 Khartoum North, Sudan
2UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia
Serdang, 43400, Selangor, Malaysia
3Centre of Natural Products and Drug Discovery (CENAR), Department of Pharmacology, Faculty of
Medicine,
University of Malaya, 50603 Kuala Lumpur, Malaysia
1
ABSTRACT
Background. We extracted phenolic compounds from Annona squamosa (leaves, bark, roots and seedcake),
and Catunaregam nilotica (leaves, bark and seedcake) using methanol and their antioxidant activity was
evaluated employing various established in vitro systems.
Material and methods. Annona squamosa (leaves, bark, roots and seedcake), and Catunaregam nilotica
(leaves, bark and seedcake) were used in the study. Antioxidant activity was estimated using oxygen radical
absorbance capacity, MTT assay and DPPH assays, and polyphenols profi le was determined by HPLC
method.
Results. The total phenolic content was determined by Folin-Ciocalteu method and the highest amounts were
171.5, 170.4, 169.5, and 167.9 g/kg plant extract as GAE for A. squamosa roots, C. nilotica bark, C. nilotica
leaves, and A. squamosa bark, respectively. The leaves extracts of the two trees showed high fl avoniod content.
The results showed that C. nilotica and A. squamosa extracts displayed antioxidant activities, with IC50
values ranging from 7.81 to 62.5 and from 7.81 to 125.0 μg/ml, respectively using 1,1-diphenyl-2-picrylhydrazyl
(DPPH) assay. The different parts extracts from two trees showed good antioxidant activity evaluated
by oxygen radical absorbance capacity and MTT assay systems.
Conclusion. These results suggested that Annona squamosa and Catunaregam nilotica phenolic compounds
could be utilized as a natural antioxidant.
Key words: Annona squamosa, antioxidant activity, Catunaregam nilotica, 1,1-diphenyl-2-picrylhydrazyl
(DPPH), oxygen radical absorbance capacity (ORAC), cytotoxic activity, MTT assay