Important notes:
Do NOT write outside the grey boxes. Any text or images outside the boxes will be deleted.
Do NOT alter the structure of this form. Simply enter your information into the boxes. The form will be
automatically processed – if you alter its structure your submission will not be processed correctly.
Do not include keywords – you can add them when you submit the abstract online.
Title:
Fluorescence characterization of drug carrier systems formed by drug-silver nanoparticles
complexes loaded on nanostructured PBS polymer
Authors & affiliations:
Paz Sevilla1,2, Elisa Corda2, Margarita Hernandez2, Michela Soccio2, José V. García-Ramos2,
Concepción Domingo2, Mari Cruz García-Gutierrez2
1
Departamento de Química Física II, Facultad de Farmacia, Universidad Complutense de Madrid, 28040
Madrid, Spain
2
Instituto Estructura de la Materia, IEM-CSIC, Serrano 121, 28006 Madrid, Spain
Abstract: (Your abstract must use Normal style and must fit in this box. Your abstract should be no longer than 300 words. The
box will ‘expand’ over 2 pages as you add text/diagrams into it.)
A wide variety of modern drug delivery systems developed nowadays is based on nanotechnology. The
success of these designs implies minimum of drug loss in the way to disease cells, good recognition of
such cells and maximum efficacy in therapeutic effects. In most cases they consist of biocompatible and
biodegradable nanostructured materials, like mesoporous silica, capable of include drugs and recognition
molecules. One problem associated derives from the use of external molecules acting as linkers when
molecules cannot be immobilized in the interior of nanostructures. PBS (polybutylene succinate) is an
aliphatic polyester characterized by good mechanical properties and thermal stability but, more
importantly, it is biodegradable and biocompatible. Silver nanoparticles present two principal advantages,
firstly they act as linkers for the drug to be immobilized in the interior of the nanostructured polymer and
secondly they permit an enhancement of the spectroscopic signal by the localized surface plasmon
resonances (LSPR) such nanoparticles support.
We have fabricated high aspect ratio PBS nanofibers with nanospaces between them at the upper layer.
Two different fabrication methods and two diameters of the nanofibers have been tested. The
nanostructured fibers have been subsequently loaded with drug molecules. We have used two different
molecules, emodin, an anti-tumoral drug, and ketorolac, a non-steroidal anti-inflammatory drug (NSAID).
Prior to load, these molecules have been adsorbed on colloidal silver nanoparticles. Fluorescence
characterization of the carrier systems has been achieved by recording Metal Enhanced Fluorescence
(MEF) spectra of cross-sections of layers taken with 1 μm spatial resolution. These spectra have been
used for mapping the penetration of the drug molecules in the layer. Our results show that the drugnanoparticle complexes remain included in the porous polymer layers, but their penetration is not
uniform. These results constitute preliminary studies necessary to design more sophisticated drug delivery
systems that are now in progress in our group.