Table 3 Kinetics of the anti-OVA antibodies production before (day 0) and 4, 9, 14 and 17 days after ovalbumin injection of fattening lambs
assessed by an indirect ELISA. The results are expressed as an index of optical density (I.O.D.), obtained after dividing the mean optical density
(O.D.) of each sample, by the mean O.D. of the positive control from each plate
Treatment
Day
CTRL
FS
s.e.d.
QCT
FS-QCT
0
w
w
w
w
4
w
w
w
0.081
wx
9
x
x
0.150ab
x
0.176ab
x
14
y
y
0.271a
y
0.352b
y
17
y
z
0.360a
z
0.423b
y
0.072
0.078
0.219b
0.385b
0.413b
0.078
0.078
0.073
0.072
P-value
FS×QCT
DAY
FS
QCT
FS×QCT
DAY
0.0239
0.0173
0.012
0.586
0.905
<0.001
0.075
0.133a
0.300a
0.326a
CTRL = control group (no antioxidants, 34 g palm oil kg-1 TMR); FS = flaxseed group (85 g flaxseed kg-1 TMR); QCT = quercetin group (CTRL
plus 2 g quercetin kg-1 TMR); FS-QCT = flaxseed plus quercetin group (85 g flaxseed plus 2 g quercetin kg-1 TMR); s.e.d. = standard error of the
difference; a,b Different superscripts in the same row indicate statistical differences (P<0.05) between treatments; w,x,y,zDifferent superscripts in
the same column indicate statistical differences (P<0.05) between days; P-value = probability of significant effects of flaxseed (FS), quercetin
(QCT), their interaction (FS×QCT) and day (DAY).