Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Student ID#:128180 Student Name Team Name: Super Awsomes Lab Assignment #: 5 Lab Title: Muscle Fatigue Date: 2014-04-01 Purpose / Objective(s): Take measurements in the lab of effects to heart rate and respiratory rate before and after lifting three different weights in an attempt to achieve muscle fatigue Hypothesis (ese): Assumption that there will eventually be a point where my body will be unable to continue performing a specific function, i.e., doing a bicep curl, after a specific amount of time, weight, or reps After doing a large number of reps, I assume my heart rate will increase Respiratory rate should increase theoretically after a high number of reps with a large weight Men theoretically will be able to lift heavier weights, do more reps, and have higher heart rates and respiratory rates Materials / Subjects / Specimens: Materials used included free weights at different weights, such as 3 pounds, 5 pounds, 7 pounds Data was recorded for 5 human subjects Methods / Tools / Instrumentation / Procedures: Exercise repetitions or reps were counted during a 60 second period using the wall clock or an iPhone timer Heart rate (BPM or beats per minute) was measured at resting/before muscle fatigue test, and after repetitions, and at 3 minute, 6 minute and 10 minute intervals, for three different weights Respiratory Rate (RR: amount of breaths in and out per minute) was measured at resting/before muscle fatigue test, and after repetitions, and at 3 minute, 6 minute and 10 minute intervals, for three different weights Page 1 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Results: Tables Table #1 summarizes: Heart Rate for myself (Cathy Odom) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Table #2 summarizes: Respiratory Rate (RR or breaths in and out in one minute) for myself before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Table #3 summarizes: Heart Rate for my four classmates Nancy, Christina, Krystin, and Kaycee before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Table #4 summarizes: Respiratory Rate for my four classmates Nancy, Christina, Krystin, and Kaycee before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Table #5 summarizes: Average of 5 people for Heart Rates (BPM or beats per minute) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Table #6 summarizes: Average of 5 people for Respiratory Rates (RR or breaths in and out in one minute) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Results: Graphs Figure #1 shows a graph of correlation between work and heart rate at peak performance for myself. Figure #2 shows a graph of heart rates at resting, peak, and 10 minutes post peak. Figure #3 shows a graph of maximum number of repetitions at low weight for 5 students. Figure #4 shows a graph of respiratory rates pre-stress, peak, and 6 minutes post stress. Figure #5 shows a graph of correlation between number of repetitions and resistance (weights in pounds). Figure #6 shows a graph of respiratory rates pre-stress, peak, and 6 minutes post stress. Page 2 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Analysis: Tables Table #1 summarizes heart rate for myself (Cathy Odom) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes. With a low weight of 2 pounds, I was able to do a high number of repetitions at 44, but at a 7 pound weight, I was only able to do 29 reps. My heart rate was actually lower at 10 minutes post workout at 60 than before starting at rest at 76 beats per minute. Table #2 summarizes respiratory rate (RR or breaths in and out in one minute) for myself before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes. My respiratory rate started at 15 pre-stress, and had a peak at 23, and at 10 minutes post peak it was at 17. Table #3 summarizes heart rate for my four classmates Nancy, Christina, Krystin, and Kaycee before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes. Heart rates averaged per person widely varied from 79 to 98 pre-stress. Heart rate at peak average varied from 78-84. Heart rates at 10 minutes post peak average per person varied from 74-76, a much closer statistic. Table #4 summarizes respiratory rate for my four classmates Nancy, Christina, Krystin, and Kaycee before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes. All four women each used weights here of 3 pounds, 7 pounds, and 15 pounds. Respiratory rates pre stress were either 19 of 20 for all four women. Respiratory rates at peak interval average varied from 22-28 breaths per minute. Respiratory rates at 6 minutes post cardiac stress varied from 18-25 breaths per minute. Table #5 summarizes an average of 5 people for Heart Rates (BPM or beats per minute) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes. Weight reps for the low weight averaged a high number at 47, and for the medium weight at 41, and then 26 at the highest weight. So as the weight increased, the number of repetitions decreased. Table #6 summarizes the average of 5 people for Respiratory Rates (RR or breaths in and out in one minute) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes. Respiratory rates at peak fatigue varied from 26-39 breaths per minute. Respiratory rates at 3 minutes post peak were very close together, and averaging at 21.3 breaths per minute. Respiratory rates at 10 minutes post peak varied from 18-21 breaths per minute with an average of 20.3. So, respiratory rates followed a pattern of being higher at peak and lower post peak. Analysis: Graphs Figure #1 shows a graph of a correlation between work and heart rate at peak performance for myself. This shows a correlation, that as your heart rate goes up, your work rate also goes up as well. Figure #2 shows a graph of heart rates at resting, peak, and 10 minutes post peak. At resting, heart rates were low, and at peak, heart rates were high, and then decreasing by 10 minutes post peak for most students. Figure #3 shows a graph of maximum number of repetitions at low weight for 5 students. These varied closely from 44 to 51. At a lower weight, we were able to do more repetitions per minute. At a higher weight, we were able to do less repetitions, due to muscle fatigue. Page 3 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Figure #4 shows a graph of respiratory rates pre-stress, peak, and 6 minutes post stress for 5 students. Most students, like myself, showed a pattern of a low number pre-stress, a high number at peak, and a medium number at the 6 minute post stress level. Christina showed the same respiratory rate for all three levels of pre-stress, peak, and 6 minutes post stress. I’m not sure if this means there was an error in recordkeeping or if this is a different type of heart/cardiac activity, that her heart was not stressed during this activity. Figure #5 shows a graph of the theoretical correlation between number of repetitions and resistance (weights in pounds). Theoretically, at a higher number of weight resistance, there should be less number of repetitions. This scatter plot did not quite come in at a perfect linear line, so I’m not sure if I did this one incorrectly or if the data is supposed to plot in this manner. Figure #6 shows a graph of the correlation between work and peak respiratory rates. Theoretically, as work increases, so should respiratory rates. The scatter plot and linear line seemed to show a better correlation in this graph than in Figure #5. Page 4 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL ATTACHMENTS Summary / Formal / Conclusive Results / Tables, Charts, Illustrations Table #1 shows Heart Rate for myself (Cathy Odom) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Self/Cathy Weight (LBS) Reps for 1 Minute or Fatigue Work Ht Rate Pre Stress Ht Rate at Peak / Fatigue Ht Rate Post Stress 3 Minutes Ht Rate Post Stress 6 Minutes Ht Rate Post Stress 10 Minutes Low Medium High 2.0 3.0 7.0 44 41 29 88.0 123.0 203.0 76 80 78 100 100 100 64 72 70 56 68 64 60 64 60 Average 4.0 38.0 152.0 100.0 78.0 68.7 62.7 61.3 Table #2 shows Respiratory Rate (RR or breaths in and out in one minute) for myself before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Self/Cathy Resp Rate Pre Stress Resp Rate at Peak / Fatigue Resp Rate Post Stress 3 Minutes Resp Rate Post Stress 6 Minutes Resp Rate Post Stress 10 Minutes Low Medium High 15 15 15 20 22 23 19 22 21 18 20 21 17 26 20 Average 15.0 21.7 20.7 19.7 21.0 Page 5 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Table #3 shows Heart Rate for my four classmates Nancy, Christina, Krystin, and Kaycee before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Reps for 1 Ht Rate at Ht Rate Post Ht Rate Post Ht Rate Post Nancy Weight Ht Rate Pre Minute or Work Peak / Stress Stress 6 Stress Weight LB Stress Fatigue Fatigue 3 Minutes Minutes 10 Minutes Low 3.0 51 153.0 88 80 78 76 76 Medium 7.0 44 308.0 88 80 76 76 74 High 15.0 24 360.0 88 88 78 78 78 Average 8.3 39.7 330.6 88.0 84.7 84.7 76.7 76.0 Christina Weight LB Low Medium High Average 3.0 7.0 15.0 8.3 Krystin Weight LB Low Medium High Average 3 7 15 8.3 Kaycee Weight LB Low Medium High Average 3.0 7.0 15.0 8.3 Reps for 1 Minute or Fatigue 51 44 24 39.7 Reps For 1 Minute or Fatigue 47 36 25 36.0 Reps for 1 Minute or Fatigue 44 41 30 38.3 Work Ht Rate Pre Stress 153.0 308.0 360.0 330.8 90 98 98 98.0 Ht Rate at Peak / Fatigue 60 80 108 84.7 Ht Rate Post Stress 3 Minutes 78 86 90 84.7 Ht Rate Post Stress 6 Minutes 80 86 86 84.0 Ht Rate Post Stress 10 Minutes 80 84 84 82.7 Work Heart Rate Pre Stress Heart Rate at Peak 88 90 88 88.7 Ht Rate Post Stress 3 Minutes 80 74 84 79.3 Ht Rate Post Stress 6 Minutes 76 73 72 73.7 Ht Rate Post Stress 10 Minutes 78 75 74 75.7 141.0 287.0 450.0 319.4 80 78 80 79.3 Work Ht Rate Pre Stress Ht Rate at Peak / Fatigue 74 75 86 78.3 Ht Rate Post Stress 3 Minutes 70 73 82 75.0 Ht Rate Post Stress 6 Minutes 70 73 80 74.3 Ht Rate Post Stress 10 Minutes 72 72 80 74.7 132.0 287.0 450.0 319.4 80 78 80 79.3 Page 6 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Table #4 shows Respiratory Rate for my four classmates Nancy, Christina, Krystin, and Kaycee before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Resp Rate Post Weight Resp Rate Resp Rate at Resp Rate Post Resp Rate Post Stress Nancy Stress LB Pre Stress Peak / Fatigue Stress 6 Minutes 10 Minutes 3 Minutes Low 3.0 20 22 20 18 18 Medium 7.0 20 28 26 28 26 High 15.0 20 36 32 30 28 Average 8.3 20.0 28.7 26.0 25.3 24.0 Christina Weight LB Resp Rate Pre Stress Resp Rate at Peak / Fatigue Low Medium High Average 3.0 7.0 15.0 8.3 20 20 20 20.0 20 22 26 22.7 Krystin Weight LB Resp Rate Pre Stress Resp Rate at Peak / Fatigue Low Medium High Average 3 7 15 8.3 20 19 18 19.0 Kaycee Weight LB Low Medium High Average 3.0 7.0 15.0 8.3 Resp Rate Post Stress 3 Minutes 22 24 22 22.7 Resp Rate Post Stress 6 Minutes Resp Rate Post Stress 10 Minutes 20 20 20 20.0 18 20 20 19.3 Resp Rate Post Stress 3 Minutes Resp Rate Post Stress 6 Minutes Resp Rate Post Stress 10 Minutes 26 26 25 25.7 18 20 25 21.0 16 18 22 18.7 17 17 20 18.0 Resp Rate Pre Stress Resp Rate at Peak / Fatigue Resp Rate Post Stress 3 Minutes Resp Rate Post Stress 6 Minutes Resp Rate Post Stress 10 Minutes 18 19 20 19.0 30 24 30 28.0 22 22 20 21.3 18 20 20 19.3 20 18 19 19.0 Page 7 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Table #5 shows an average of 5 people for Heart Rates (BPM or beats per minute) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Work Ht Rate Pre Stress Ht Rate at Peak / Fatigue 47.4 41.2 26.4 132.7 287.0 450.0 87.6 88.4 89.2 75.6 81.0 89.6 Ht Rate Post Stress 3 Minutes 74.0 76.2 80.8 38.3 319.4 88.9 82.9 78.5 Weight LBS Reps for 1 Minute or Fatigue Low Medium High 2.8 6.2 13.4 Average 7.5 Weight / Resistance Level Ht Rate Post Stress 6 Minutes Ht Rate Post Stress 10 Minutes 71.6 75.2 76.0 73.2 73.8 75.2 74.3 74.1 Table #6 shows an average of 5 people for Respiratory Rates (RR or breaths in and out in one minute) before bicep curls and at peak, and at intervals after of 3 minutes, 6 minutes, and 10 minutes Weight / Resistance Level Weight LBS Resp Rate Pre Stress Resp Rate at Peak / Fatigue Resp Rate Post Stress 3 Minutes Resp Rate Post Stress 6 Minutes Resp Rate Post Stress 10 Minutes Low 2.8 12.6 30.1 22 18 18 Medium 6.2 7.9 39.0 22 21.2 21.4 High 13.4 13.4 26.4 20 22.6 21.4 Average 8.3 11.3 31.8 21.3 20.6 20.3 Page 8 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Figure #1: Graph of Correlation between Work and Heart Rate at Peak Performance (self) Page 9 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Figure #2: Graph of Heart Rates at Resting, Peak, and 10 Minutes Post Peak for 5 Students Page 10 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Figure #3: Graph of Maximum Number of Repetitions at Low Weight for 5 Students Page 11 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Figure #4: Graph of Respiratory Rates Pre-Stress, Peak, and 6 Minutes Post Stress Page 12 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Figure #5: Graph of Correlation Between Number of Repetitions and Resistance (Weights in Pounds) Page 13 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL Figure #6: Graph of Correlation Between Work and Peak Respiratory Rates Page 14 of 15 401288112 Biology 105 – Human Biology Spring 2014 55244 4 Units UVC1 St. Helena F 9:00 AM – 3:50 PM RIDDELL References 1. How to use correlations in Excel, website reference http://www.gifted.uconn.edu/siegle/research/correlation/excel.htm 2. How to use correlations in Excel, YouTube video reference https://www.youtube.com/watch?v=WgQmC_VfwO8 Observations/Conclusions / Further Considerations: 1. At the highest weight, it was getting harder and harder for me to do repetitions on the last weight, until I got to a point where I could no longer lift the weight in a bicep curl. For the 7 pound weight, I was only able to do 29 repetitions in 1 minute and my arm was quite fatigued to the point of no longer being able to move. At heavier weights for all students, we all did less repetitions, as our muscle was getting fatigued. 2. Theoretically, I believe men would lift heavier weights and do more reps. However, this lab and the previous has also shown, that it’s very hard to get data from male lab partners in a consistent manner. We asked for data via email and on the Facebook website. When we saw a lone wolf male in our group study meeting, alas, he brought his computer, but not his notebook, where his data was. Ah, the male lab data is quite elusive this spring season. 3. As you are working out, your heart rate, respiratory rate and work will increase to a peak level, and then drop off at different time levels until your body is back at rest. This usually follows a pattern for most students, but not all. This anomaly could be record keeping errors, such as transposed numbers, or perhaps different levels of cardiac abilities per person which will differ. 4. For students with good cardiac state and student athletes, they should be able to recover quickly, lift heavier weights and do more repetitions that those of us that are “couch potatoes”. On future labs, we can also collect data on students workouts/week and body fat percentages to muscle to compare and contrast student data. 5. As you increase the weight used in the bicep curl, the repetition totals will slow, but the heart rate will actually increase, as it is working harder to lift the weight. This makes sense as the heart is pumping faster. Page 15 of 15 401288112