Principle of
Physiology
(NRS 237)
Practical
T. Souad Bouna
 Bachelor in Lab Medical Technology, 7/2013
Taibah University, College of Applied Medical Science.
 s.boune@mu.edu.sa
 Schedule (Day/Time):
Thurs / 10:00 – 11:50
Wed / 12:30 – 2:20
 Room:
Physiology Lab
 Grading :
20 marks
10 marks / final
5 marks /attendance
5 marks /activity
What is Physiology ?
Study of the functioning of living organisms or their
constituent tissues or cells.
An understanding of biochemistry is fundamental to
physiology.
Physiological processes are dynamic; cells change their
function in response to changes in the composition of their
local environment, and the organism responds to
alterations in both its internal and external environment.
Many physiological reactions are aimed at preserving a
constant physical and chemical internal environment
(homeostasis)
Physiology Lab:
 Determination of blood pressure in human
( how to use the stethoscope and sphygmanometer ).
 Observation of the effect of the position and exercise on
cardiac activity ( blood pressure & pulse rate ).
 Determination of bleeding time.
 Determination of clotting time.
 Estimation of hemoglobin (Hb) concentration in gm/
100ml of blood.
 Find out your own blood group.
 Counting of RBCs by using HEMOCYTOMETER.
 Counting of WBCs by using HEMOCYTOMETER.
Lab Safety Rules:

Do NOT bring food or drink into the lab. Chewing gum, applying
cosmetics, putting pens or pencils into your mouth

Always wear shoes in the lab.

Use proper safety protection --- lab coat , goggles, gloves.

Keep the laboratory as clean and neat as possible at all times. Put extra
books, purses, coats, etc. well away from the work area.

Clean spills from the outside IN. Apply paper towels over the spill, then,
carefully starting from the outside, wipe in.

Replace chairs at lab stations as you leave.

Properly dispose of trash.

Pay attention to special instructions regarding clean-up of equipment or
disposal of supplies.
 Properly dispose of trash.
 Pay attention to special instructions regarding clean-up
of equipment or disposal of supplies.
 Report any defects in your microscope or other
equipment immediately to your instructor.
 Read all instructions before beginning the lab.
 Learn the location of the fire-extinguisher and first-aid
kit.
Report any accident, injury, or unusual event to the
instructor, no matter how insignificant it may seem.
 Keep electrical equipment far from water, keep areas
around electrical equipment dry.