15th Iranian Seminar of Analytical Chemistry
Feb, 27 – March, 1, 2007
Chemistry Department
Shiraz University
Evaluation Procedures for Liquid-Liquid Microextraction and Spectrophotometric Analysis of
Three Carcinogenic Aromatic Amines Using Mean Centering of Ratio Spectra and Partial Least
Squares
Methods
Tahereh Heidaria , Hakimeh Assadia and Zarrin Es’haghib*
a
b
Department of Chemistry, Faculty of sciences, Ferdowsi University, Mashhad, Iran
Department of Chemistry, Faculty of sciences, Payame Noor University, Iran.
Despite of their toxic and carcinogenic properties, aniline and their derivatives are important because of
wide use in many industrial processes. Therefore monitoring of their levels in environmental waters is
important. These compounds have been included in the US environmental protection agency (EPA) list
of priority pollutants. Many of these amines are known to be highly mutagenic and carcinogenic and to
from adducts with proteins and DNA [1]. These amines are present in the environment at low parts per
billion or less. Therefore a certain clean – up procedure for the sample matrix is necessary. Thus
extraction and clean – up of the sample has been performed using a number of different purification
techniques such as Solid Phase Microextraction (SPME) [2] and Liquid Phase Microextraction
(LPME) [3-6]. In the present research liquid – liquid microextraction method was illustrated for the
extraction of a mixture of 3 aniline derivatives; N-ethyl aniline, 2-nitroaniline and 2-bromoaniline from
water, using spectrophotometry to determine the concentrations of extracted components, but the
mixture showed overlapping spectra. Thus, for resolving mixtures of these components we have used
of two simple and efficient methods. Mean Centering of Ratio Spectra and Partial Least Squares [7,8].
Finally, in order to evaluate the practical application of the method, recovery were determined
and extraction precision, linearity and limits of detection (LOD’s) for selected analytes were
evaluated.The results obtained in this work indicate the development and application of a novel method
approach to extracting and determining aniline derivatives from water samples.
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