Microbiology Unknown Lab
BIO 187
0.1%
dextrose
TSI agar
1.0%
sucrose
Triple Sugar Iron Agar
A
(a) Red/red (no sugar
fermentation)
(b) Control
(c) Red/yellow (Glucose
fermented but lactose and
sucrose not fermented)
(d) Yellow/yellow (Glucose
fermented. Lactose and/or
sucrose fermented)
(e) Red/yellow with H2S
B
C
D
E
1.0%
lactose
Penol Red broths
A
B
C
D
E
A (acid with gas)…..B (acid)……C (uninoculated control)……D (no recation)… …E
(alkaline reaction)
O-F tests
Control
E. coli
Fermenter
Pseudomonas
Oxidizer
Alcaligenes
Nonsaccharolytic
Motility Test
Decarboxylation
uninoculated
Positive
Negative
Gelatin Hydrolysis
Simmons Citrate
A
B
C
A: Positive…Enterobacter
B: Negative…E. coli
C: Control-uninoculated
Phenylalanine Deaminase Test
neg
pos
Urea Hydrolysis
Positive
uninoculated
Negative
Methyl Red Test
A
A: Positive (E. coli)
B
B: Negative (Enterobacter aerogenes)
Voges Proskauer Test
A
A: Negative (E. coli)
B
B: Positive (Enterobacter aerogenes)
Indole Test
Negative
-
Positive
+
Catalase Test
+
Positive
Negative
GRAM
STAIN
General Microbiology (BIOL 187) Laboratory Report (Unknown Identification)
Page 1: Cover page
Include your name, the unknown number and you final Genus and species identification
Page 2: Test listing
I want a (+) / (-) listing of each test in a vertical, easily scanned format
Page 3: Discussion section
Detail, in complete sentences, why your results justify your identification and rule out other possibilities.
Page 4: About My Microbe section
Research your microbe and give me a several paragraphs about your microbe.
(Don't copy word-for-word from your textbook---This will be frowned upon!)
Page 5: Dichotomous key
Create a neat dichotomous key, which results in the correct separation of all 8 possible unknowns. This key should be
legible and sized to occupy a complete sheet of paper (nothing microscopic is acceptable here)
Page 6:Lab handout where you recorded your initial results
Include the handout from lab where your recorded your data as you read the test reactions.
Grading:
20 pts.
Correct Identification
10 pts.
Discussion section
7 pts.
"About my microbe section"
8 pts.
Dichotomous key
5 pts.
Neatness, accuracy and completeness of report
50 points total