ENVIRONMENTAL RISK MANAGEMENT AUTHORITY
DECISION
Amended under s67A on 22 August 2007
26 March 2001
Application code
GMC00001
Application type
To import into containment a genetically modified organism under
section 40(1)(a) of the Hazardous Substances and New Organisms
(HSNO) Act.
Applicant
New Zealand Institute for Crop and Food Research (Crop and Food
Research)
Purpose
Date received
To gain approval to maintain in containment and to import strains of
genetically modified E. coli and Agrobacterium which are used to facilitate
the introduction of foreign DNA into a wide range of crop species.
6 November 2000
Consideration period
13 February 2001 – 22 February 2001
Considered by
GMO (Genetically Modified Organisms) New Organisms Standing
Committee of the Environmental Risk Management Authority (the
Authority).
Decision
The application is approved subject to controls in accordance with sections 45(1)(a) and 45(2)
of the HSNO Act 1996.
The organisms approved are 142 strains of genetically modified Escherichia coli, Agrobacterium
tumefaciens and A. rhizogenes as listed in Annex 1 to this decision.
Application process
The application was formally received on 06 November 2000 and verified to contain sufficient
information to be processed on 17 November 2000. The application was stalled under section 58
of the Act from 5 December 2000-15 January 2001 while the scope of the organisms for
importation was clarified. Public notification of the application was not required under the
Hazardous Substances and New Organisms (HSNO) Act 1996.
The documents available for the evaluation and review of the application by ERMA New
Zealand included the application and appendices listing the organisms.
The application was considered by the Genetically Modified Organisms Standing Committee of
the Authority appointed in accordance with section 19(2)(b) of the HSNO Act. The Committee
comprised the following members: Ms Jill White, Professor Colin Mantell and Dr Oliver
Sutherland.
The application included a request for approval for a modified strain of Agrobacterium rubi. This
strain was later confirmed to be a non-modified strain with an existing approval for import into
physical containment level 2 (PC2) (approval code: NOC001663). The Committee confirmed that
no additional approval of A. rubi is required. The full details of the controls on A. rubi are
included in the decision on application NOC99014.
Relevant legislative criteria
The application was lodged pursuant to section 40(1)(a) of the HSNO Act. The decision was
determined in accordance with section 45, taking into account additional matters to be
considered under sections 37 and 44 and matters relevant to the purpose of the Act, as specified
under Part II of the Act.
Consideration of the application followed the relevant provisions of the Hazardous Substances
and New Organisms (Methodology) Order 1998, but with particular regard to clauses 8 (dealing
with the scale and significance of the risks, costs and benefits) and 26 (dealing with applications
where the risks are negligible).
Reasons for the decision
Purpose
The Committee considers that the purpose of the application is to gain approval under section 40
of the HSNO Act 1996 for existing bacterial collections and for additional A. tumefaciens strains
intended for import into containment for research purposes. In accordance with section
45(1)(a)(i) of the HSNO Act, the Committee determined that this was an appropriate purpose
under section 39(1)(h) of the Act 1996: Such other purposes as the Authority thinks fit.
Inseparable organisms
The Committee has considered the effects of any inseparable organisms, in accordance with
section 45(a)(ii) of the HSNO Act and noted that the organism would be imported in the form of
pure cultures, therefore contamination with other organisms is very unlikely. Should any
inseparable organisms be imported, it is very unlikely they would escape containment under the
specified containment controls imposed in this decision.
Ability to escape containment
In accordance with section 44(b) of the HSNO Act, the Committee considered the ability of
the organisms to escape from containment.
Containment controls to which the approval of this application is subject are considered to
satisfactorily address the matters detailed in the Third Schedule Part I, Containment Controls
for Developing, Importing or Field Testing of Genetically Modified Organisms of the HSNO
Act.
The Committee requires the organisms to be maintained in a facility that is registered and
operated according to the Ministry of Agriculture and Forestry (MAF) Biosecurity
Authority/ERMA New Zealand Standard 154.03.02: Containment Facilities for
Microorganisms and the Australian and New Zealand Standard AS/NZS 2243.3:1995 Safety
in Laboratories: Part 3: (Microbiology), at Physical Containment Level 1 (PC1).
Environmental Risk Management Authority Decision: Application GMC00001
Page 2 of 8
The Committee is satisfied that it is very unlikely that bacterial strains listed in Appendix 1 could
escape containment, taking into account the laboratory procedures proposed by the applicant and
the containment controls imposed in this decision.
The Committee notes that the applicant is required to implement procedures for the retrieval or
destruction of any viable material of the organism that has breached containment, as part of the
facility approval, and the procedures shall be detailed in the facilities containment manuals, which
require MAF approval.
Ability to form self-sustaining populations and ease of eradication
In accordance with sections 37(a) and (b) of the HSNO Act, the Committee considered the
ability of the organisms to establish a self-sustaining population and the ease with which any such
populations could be eradicated.
Escherichia coli
The Committee notes that the E. coli are derivatives of strain K12, which has been demonstrated
to be unable to establish a self-sustaining population outside of laboratory culture (Smith 1975,
Heitkamp et al. 1993) The Committee considers that in the very unlikely event of escape, the E.
coli are very unlikely to establish self-sustaining populations. The Committee considers eradication
of the organisms would be difficult, should escape occur and a population establish. Also, any
eradication method is likely to adversely effect the microorganisms already present in that
environment.
Agrobacterium tumefaciens and Agrobacterium rhizogenes
The Committee notes that despite the widespread use of A. tumefaciens in laboratory research no
information was provided by the applicant or was found by ERMA New Zealand on the risk
assessment of Agrobacterium laboratory strains.
Survival of A. tumefaciens strains on plants that have been genetically modified by the bacteria
have been reported (e.g., Matzk et al. 1996), therefore the Committee considers that at least some
laboratory strains of A. tumefaciens will be able to survive outside of containment.
No information on the ability of A. rhizogenes strains to survive outside of containment was
available for the consideration, but since these strains are also used to infect plants it is
considered likely that they could survive if they escaped.
The Committee notes that A. tumefaciens and A. rhizogenes strains would have to compete with
other Agrobacterium species that naturally inhabit the plant rhizosphere. However, the Committee
considers that there is uncertainty over whether the genetically modified laboratory strains of A.
tumefaciens and A. rhizogenes will be able to establish self-sustaining populations in the very unlikely
event that they should they escape.
Environmental Risk Management Authority Decision: Application GMC00001
Page 3 of 8
Adverse effects
The Committee considered potential adverse effects of the organisms, in accordance with section
45(a)(ii) of the HSNO Act.
Effects on human health
The Committee considers that it is very unlikely that the genetically modified laboratory strains of
E. coli, A. tumefaciens and A. rhizogenes will have adverse health effects.
It is noted that all three species are included in Schedule 2 of HSNO (Low-Risk Genetic
Modification) Regulations as approved host/vector systems. Modification to such organisms are
considered low risk and require PC1 containment, in accordance with the Australian and New
Zealand Standard AS/NZS 2243.3:1995 Safety in Laboratories: Part 3: (Microbiology).
Effects on the environment
Escherichia coli
The Committee notes that E. coli strains derived from strain K-12 are very widely used in New
Zealand as well as overseas laboratories. The Committee are not aware of any adverse effects
associated with their maintenance or their escape from containment. Consequently, in the event
of escape from containment the Committee considers that it is very unlikely that these E. coli
strains will have adverse environmental effects.
Agrobacterium tumefaciens and Agrobacterium rhizogenes
A. tumefaciens is a widespread soil bacterium that can colonise and infect a broad range of
dicotyledonous plants. Virulent strains produce special compounds that induce tumour formation
in host plants. The Committee notes that these virulent determinants are lacking in the laboratory
strains listed in this application so that it is very unlikely that tumours will be formed if these
strains infect plants.
The applicant noted that the plant pathogenic A. tumefaciens strains are all disarmed and non-gall
forming. However, these strains are likely to be still able to colonize plants, and transfer genetic
material to them via their Ti plasmid, so that in the event of an escape from containment and
plant colonisation genetic material may be transferred to some of the cells of the plant(s). The
magnitude of such an effect will depend upon the genetic material transferred, the number and
types of plant cells modified, and the plant species involved.
A. rhizogenes is also a widespread soil bacterium that can colonise the roots of a range of
dicotyledonous plants. Wild type strains of A. rhizogenes can induce rootlet proliferation in plants,
resulting in “hairy root” syndrome. It is uncertain whether the A. rhizogenes strains to be imported
will have the root-inducing capability of the wild strains. They will, however, have the ability to
transfer genetic material to plants.
The Committee notes that during the evaluation of the application ERMA New Zealand
searched for but did not find reports of adverse effects associated with the escape of A.
tumefaciens or A. rhizogenes strains from laboratories. A. tumefaciens has been used for many years in
New Zealand laboratories so that adverse effects associated with escapes from containment
could be expected to have been observed.
Environmental Risk Management Authority Decision: Application GMC00001
Page 4 of 8
In the event of escape from containment the Committee considers that there is the potential for
the A. tumefaciens and A. rhizogenes strains to have adverse environmental effects. This is
dependent upon them colonising a plant and transferring foreign genetic material to the plant.
The magnitude of an effect will depend on the nature of the genetic material transferred and the
number of plants infected. As noted above, there is uncertainty over the ability of the laboratory
strains to establish an infection outside of containment. The Committee notes that the A.
tumefaciens and A. rhizogenes strains are approved Schedule 2 hosts in the HSNO (Low-Risk
Genetic Modification) Regulations and considers that the containment controls imposed by this
decision make it very unlikely that Agrobacterium will escape from containment.
Effects on the relationship of Māori with their taonga
The Committee considers adverse effects on Māori culture are very unlikely given that the
organisms are to be used as laboratory tools in containment.
The application is not for the development of GMOs but for the maintenance of existing
collections, for their reimportation and for the import of additional strains from overseas
sources. The Committee notes that use of the bacteria in modifying plants requires separate
approvals to be gained for development of a GMO in containment.
Negligible risk
Based on the consideration and analysis of adverse effects on the environment and public health,
but particularly taking into account the containment controls imposed in this decision, the
Committee considers that the risks associated with the importation of the genetically modified E.
coli, A. tumefaciens and A. rhizogenes strains, as listed in Appendix 1, into containment are negligible.
The Committee has therefore considered this application in terms of clause 26 of the
Methodology.
Costs and benefits
The Committee considers the primary benefits of this application are associated with the
increased knowledge to be gained through the use the bacteria in various genetic engineering
programmes currently being undertaken by Crop and Food Research.
The Committee is satisfied that costs are unlikely to accrue to parties other than the applicant.
Conclusion
The Committee concludes that, taking account of the ability of the organism to escape from
containment as in section 44(b) of the HSNO Act, the beneficial effects of having the organism
in containment outweigh the likely adverse effects of the organism and any inseparable
organisms, should the organism escape.
Having considered the possible effects of the organism in accordance with sections 45(1)(a)(ii)
and (iii) of the HSNO Act, the Committee is satisfied that the proposed containment regime and
additional controls can adequately contain the organism.
The application to import into containment the new organisms, as listed in Appendix 1, is thus
granted in accordance with section 45(1)(a) of the HSNO Act. As required under section 45(2)
the approval is subject to containment controls, as specified below.
Environmental Risk Management Authority Decision: Application GMC00001
Page 5 of 8
Containment controls
In order to satisfactorily address the matters detailed in the Third Schedule Part I Containment
Controls for Developing, Importing or Field Testing of Genetically Modified Organisms1 of the Act, the
Authority’s approval of this application is subject to the following controls:
1.
To limit the likelihood of any accidental release of any organism or any
viable genetic material2:
1.1
The person responsible for a particular research area and/or the person responsible for the
operation of the containment facility shall inform all personnel involved in the handling of
the organisms of the Authority’s controls.
1.2
The containment facilities shall be approved by MAF in accordance with the MAF
Biosecurity Authority/ERMA New Zealand Standard 154.03.023, and the controls of the
Authority.
1.3
The construction and operation of the containment facilities in which the genetically
modified organisms (as listed in Appendix 1 to this decision) are maintained and reimported into shall be in accordance with the:
a)
MAF/ERMA New Zealand Standard 154.03.023:Containment Facilities for
Microorganisms.
b)
Australian/New Zealand Standard AS/NZS 2243.3:19953 Safety in Laboratories:
Part 3: (Microbiology), at Physical Containment Level 1 (PC1)
2.
To exclude unauthorised people from the facility
2.1
The identification of entrances, numbers of and access to entrances, and security
requirements for the entrances and the facility shall be in compliance with the
requirements of the standards listed in control 1.3.
3.
To exclude other organisms from the facility and to control undesirable
and unwanted organisms within the facility
3.1
The exclusion of other organisms from the facility and the control of undesirable and
unwanted organisms within the facility shall be in compliance with the standards listed
in control 1.3
Bold headings refer to Matters to be Addressed by Containment Controls for Development and Field Testing of Genetically
Modified Organisms, specified in the Third Schedule of the HSNO Act 1996.
1
Viable Genetic Material is biological material that can be resuscitated to grow into tissues or organisms. It can be defined to
mean biological material capable of growth even though resuscitation procedures may be required, eg when organisms or parts
thereof are sublethally damaged by being frozen, dried, heated, or affected by chemical.
2
3
Any reference to this standard in these controls refers to any subsequent version approved or endorsed by ERMA New Zealand
Environmental Risk Management Authority Decision: Application GMC00001
Page 6 of 8
4.
To prevent unintended release of the organism by experimenters working
with the organism
4.1
The prevention of unintended release of the organism by experimenters working with the
organism shall be in compliance with the standards listed in control 1.3.
5.
To control the effects of any accidental release or escape of an organism
Control of the effects of any accidental release or escape of an organism shall be in
compliance with the standards listed in control 1.3.
If a breach of containment occurs, the facility operator must ensure that the MAF Inspector
responsible for supervision of the facility has received notification of the breach within 24
hours.
In the event of any breach of containment the contingency plan for the attempted retrieval or
destruction of any viable material of the organisms that have escaped shall be implemented
immediately. The contingency plan shall be included in the containment manual in
accordance with MAF/ERMA New Zealand Standard 154.03.023: Containment Facilities
for Microorganisms.
6.
Inspection and monitoring requirements for containment facilities
The inspection and monitoring requirements for containment facilities shall be in compliance
with the standards listed in control 1.3.
The containment manuals shall be updated, as necessary, to address the implementation of
the controls imposed by this approval, in accordance with MAF/ERMA New Zealand
Standard 154.03.023: Containment Facilities for Micro-organisms.
7.
Qualifications required of the persons responsible for implementing those
controls
The training of personnel working in the facility shall be in compliance with the standards
listed in control 1.3.
________________________________
Date: 26 March 2001
Ms Jill White, Chair
GMO New Organisms Standing
Committee of the Authority
Environmental Risk Management Authority Decision: Application GMC00001
Page 7 of 8
Amendment: November 2006
Changes to controls:
 Addition of footnotes to the containment facility references and the
Australian/New Zealand containment facility references to “future proof” the
decision
 Standardise the wording of the breach of containment control
 Removal of the control regarding inspection of facilities by the Authority, its
agent or enforcement officers
____________________________
Dr Kieran Elborough
Chair, GMO Standing Committee
Date: 22 August 2007
References
Heitkamp MA, et al. (1993). Fate in sewage of a recombinant Escherichia coli K-12
strain used in the commercial production of bovine somatotropin. J. Ind. Microbiol.
11, 243-252.
Matzk A, Mantell S, Schiemann J (1996). Localization of persisting agrobacteria in
transgenic tobacco plants. Molecular Plant Microbe Interactions 9, 373-381.
Smith HW (1975). Survival of orally administered E. coli K12 in alimentary tract of
man. Nature 255, 500-502.
Environmental Risk Management Authority Decision: Application GMC00001
Page 8 of 8
Decision Apppendix 1: GMC00001 Approved microorganisms
Organisation
Collection name
Risk Group
Crop and Food Research Limited, Lincoln and Palmerston North
E. coli, Agrobacterium tumefaciens and A. rhizogenes
Low-risk category A or B, Risk group 1
Approval
code
Common Name
Description
Taxonomic name
GMC000943
Agrobacterium rhizogenes
(A.r. 24c (R1023))
GMC00001
Agrobacterium rhizogenes
(genetically modified)
GMC000944
Agrobacterium rhizogenes
(A.r. 32) GMC00001
GMC000945
Agrobacterium rhizogenes
(A.r.18 (R1022))
GMC00001
GMC000946
Agrobacterium rhizogenes
(LBA 9402) GMC00001
GMC000947
Agrobacterium tumefaciens
(Agro= strain LBA4404+
CAT (Chloramphenicol
resistance)) GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
codA (5 fluoro-cytosine
susceptibility)) GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
HPT (hygromycin
resistance)) GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
Luc (Luciferase activity))
GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
Spec (Spectinomycin
resistance)) GMC00001
Agrobacterium tumefaciens
(Agrostrain LBA4404+ Lc
(anthocyanin regulatory
sequence leads to red
leaves)) GMC00001
Agrobacterium tumefaciens
(Chry 5 (tumourgenic))
GMC00001
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection to be imported)
CFR ref. no.: 1n
Strain and binary vector: A.r. 24c (R1023)
Source/ Origin (if available): Ex Hort Research International,
Wellesbourne
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection to be imported)
CFR ref. no.: 2n
Strain and binary vector: A.r. 32
Source/ Origin (if available): Ex Hort Research International
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection to be imported)
CFR ref. no.: 3n
Strain and binary vector: A.r.18 (R1022)
Source/ Origin (if available): Ex Hort Research International
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection to be imported)
CFR ref. no.: 1mc
Strain and binary vector: LBA 9402
Source/ Origin (if available): Ex Cornell University
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 3rb Strain and binary
vector: Agro= strain LBA4404+ CAT (Chloramphenicol resistance)
Source/ Origin (if available): From Jonathon Jones, John I
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 13rb Strain and binary
vector: Agro= strain LBA4404+ codA (5 fluoro-cytosine susceptibility)
Source/ Origin (if available): From Jens Stougaard
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 6rb Strain and binary
vector: Agro= strain LBA4404+ HPT (hygromycin resistance) Source/
Origin (if available): From George Coupland, John Innes
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 8rb Strain and binary
vector: Agro= strain LBA4404+ Luc (Luciferase activity) Source/ Origin
(if available): From Saco de Vries, Wageningen Uni
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 12rb Strain and binary
vector: Agro= strain LBA4404+ Spec (Spectinomycin resistance)
Source/ Origin (if available): From Jonathon Jones, John I
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 1rb Strain and binary
vector: Agrostrain LBA4404+ Lc (anthocyanin regulatory sequence
leads to red leaves) Source/ Origin (if available): From
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium collection held in containment) CFR ref. no.: Strain and
binary vector: Chry 5 (tumourgenic) Source/ Origin (if available): Dr M.
Daub, North Carolina, USA
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 11c Strain and binary
vector: LBA4404 (pCambia 3301) Source/ Origin (if available):
Cambia@cambia.org.au from Phillipa. Electroporated into LBA
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 12c Strain and binary
vector: LBA4404(pTOK233) Source/ Origin (if available): Toshihiko
Komari, Japan Tobacco Inc., 700 Higashibara, Toyoda, Iw
Agrobacterium tumefaciens
(genetically modified)
GMC000948
GMC000949
GMC000950
GMC000951
GMC000952
GMC000953
GMC000954
Agrobacterium tumefaciens
(LBA4404 (pCambia
3301)) GMC00001
GMC000955
Agrobacterium tumefaciens
(LBA4404(pTOK233))
GMC00001
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Agrobacterium rhizogenes
(genetically modified)
Agrobacterium rhizogenes
(genetically modified)
Agrobacterium rhizogenes
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Page 1 of 8
GMC000956
Agrobacterium tumefaciens
(MOG301 (pMOG410))
GMC00001
GMC000957
Agrobacterium tumefaciens
(ABI ( pMON 39138) CP4,
etr-1 genes) GMC00001
GMC000958
Agrobacterium tumefaciens
(ABI (confidential
information provided))
GMC00001
GMC000959
Agrobacterium tumefaciens
(ABI (pMON 11063) NptII,
etr1-1) GMC00001
GMC000960
Agrobacterium tumefaciens
(ABI (pMON 15737) NptII,
GUS, CP4) GMC00001
GMC000961
Agrobacterium tumefaciens
(ABI (pMON 26140) CP4)
GMC00001
GMC000962
Agrobacterium tumefaciens
(ABI (pMON 34018) CP4)
GMC00001
GMC000963
Agrobacterium tumefaciens
(ABI (pMON 34027) CP4,
GFP. GUS) GMC00001
GMC000964
Agrobacterium tumefaciens
(ABI (pMON 39141) CP4,
etr-1 genes) GMC00001
GMC000965
Agrobacterium tumefaciens
(ABI (pMON 39154) CP4,
gent) GMC00001
GMC000966
Agrobacterium tumefaciens
(AGL1) GMC00001
GMC000967
Agrobacterium tumefaciens
(Agli (pLN27)) GMC00001
GMC000968
Agrobacterium tumefaciens
(Agli (pTMV-GUS))
GMC00001
Agrobacterium tumefaciens
(AGLO) GMC00001
GMC000969
GMC000970
GMC000971
GMC000972
Agrobacterium tumefaciens
(Agro strains LBA4404,
ASE1, KYRT1, Agli,
GV3101+ Green
Fluorescent Protein)
GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404 +
BAR (Basta resistance))
GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+ Ac
(Maize transposon))
GMC00001
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium collection held in containment) CFR ref. no.: Strain and
binary vector: MOG301 (pMOG410) Source/ Origin (if available): Mogen
Internantional nv, Leiden, The Netherlands
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported)
CFR ref. no.:
Strain and binary vector: ABI ( pMON 39138) CP4, etr-1 genes
Source/ Origin (if available): Scotts Co, New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported)
CFR ref. no.:
Strain and binary vector: ABI (confidential information provided)
Source/ Origin (if available): Scotts Co, New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported) CFR ref. no.: Strain and binary vector:
ABI (pMON 11063) NptII, etr1-1 Source/ Origin (if available): Scotts Co,
New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported) CFR ref. no.: Strain and binary vector:
ABI (pMON 15737) NptII, GUS, CP4 Source/ Origin (if available): Scotts
Co, New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported)
CFR ref. no.:
Strain and binary vector: ABI (pMON 26140) CP4
Source/ Origin (if available): Scotts Co, New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported)
CFR ref. no.:
Strain and binary vector: ABI (pMON 34018) CP4
Source/ Origin (if available): Scotts Co, New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported) CFR ref. no.: Strain and binary vector:
ABI (pMON 34027) CP4, GFP. GUS Source/ Origin (if available): Scotts
Co, New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported)
CFR ref. no.:
Strain and binary vector: ABI (pMON 39141) CP4, etr-1 genes
Source/ Origin (if available): Scotts Co, New York & Ohio.
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported) CFR ref. no.: Strain and binary vector:
ABI (pMON 39154) CP4, gent Source/ Origin (if available): Scotts Co,
New York & Ohio.
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 18rb Strain and binary
vector: AGL1 Source/ Origin (if available):
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 10c Strain and binary
vector: Agli (pLN27) Source/ Origin (if available): From Jan Grant
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 9c Strain and binary vector:
Agli (pTMV-GUS) Source/ Origin (if available): From Jan Grant
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 17rb Strain and binary
vector: AGL0 Source/ Origin (if available):
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 2rb Strain and binary
vector: Agro strains LBA4404, ASE1, KYRT1, Agli, GV3101+ Green
Fluorescent Protein Source/ Origin (if available): From Ji
Agrobacterium tumefaciens
(genetically modified)
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 4rb Strain and binary
vector: Agro= strain LBA4404 + BAR (Basta resistance) Source/ Origin
(if available): From George Coupland, John Innes Ins
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 14rb Strain and binary
vector: Agro= strain LBA4404+ Ac (Maize transposon) Source/ Origin (if
available): From Jonathon Jones, John Innes Insti
Agrobacterium tumefaciens
(genetically modified)
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Page 2 of 8
GMC000973
GMC000974
GMC000975
GMC000976
GMC000977
GMC000978
GMC000979
GMC000980
Agrobacterium tumefaciens
(Agro= strain LBA4404+
Cre/Lox recombinase )
GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
En/Spm (Maize
transposon)) GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
FLP/ FRT recombinase)
GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
NPTII (Kanamycin
resistance)) GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
SPT (Streptomycin
resistance)) GMC00001
Agrobacterium tumefaciens
(Agro= strain LBA4404+
uidA (b glucuronidase
activity)) GMC00001
Agrobacterium tumefaciens
(C58C1, avirulent, no Ti
plasmid) GMC00001
Agrobacterium tumefaciens
(EHA105 (pMOG410))
GMC00001
GMC000981
Agrobacterium tumefaciens
(EHA105) GMC00001
GMC000982
Agrobacterium tumefaciens
(EHAL 05) GMC00001
GMC000983
Agrobacterium tumefaciens
(K1, avirulent, no Ti
plasmid) GMC00001
Agrobacterium tumefaciens
(KYRT1 Disarmed version
of strain Chry 5)
GMC00001
Agrobacterium tumefaciens
(LBA4404 (electro
competent)) GMC00001
Agrobacterium tumefaciens
(LBA4404 (pBINmgfp5ER)) GMC00001
GMC000984
GMC000985
GMC000986
GMC000987
Agrobacterium tumefaciens
(LBA4404 (pMOG410))
GMC00001
GMC000988
Agrobacterium tumefaciens
(MOG101 (pMOG410))
GMC00001
GMC000989
Agrobacterium tumefaciens
(pNE5) GMC00001
GMC000990
Agrobacterium tumefaciens
(pSLJ1111 in LBA 4404)
GMC00001
Agrobacterium tumefaciens
(pSLJ721(EIK) in LBA
4404) GMC00001
GMC000991
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 9rb Strain and binary
vector: Agro= strain LBA4404+ Cre/Lox recombinase Source/ Origin (if
available): From Gary Drews, University of Utah, US
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 15rb Strain and binary
vector: Agro= strain LBA4404+ En/Spm (Maize transposon) Source/
Origin (if available): From George Coupland, John Innes
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 10rb Strain and binary
vector: Agro= strain LBA4404+ FLP/ FRT recombinase Source/ Origin (if
available): From Gary Drews, University of Utah, U
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 5rb Strain and binary
vector: Agro= strain LBA4404+ NPTII (Kanamycin resistance) Source/
Origin (if available):
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 11rb Strain and binary
vector: Agro= strain LBA4404+ SPT (Streptomycin resistance) Source/
Origin (if available): From Jonathon Jones, John Inn
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 7rb Strain and binary
vector: Agro= strain LBA4404+ uidA (b glucuronidase activity) Source/
Origin (if available): From George Coupland, John I
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 103c Strain and binary
vector: C58C1, avirulent, no Ti plasmid Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium collection held in containment) CFR ref. no.: Strain and
binary vector: EHA105 (pMOG410) Source/ Origin (if available): Mogen
Internantional nv, Leiden, The Netherlands
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium collection held in containment) CFR ref. no.: Strain and
binary vector: EHA105 Source/ Origin (if available): Dr H. Hood, Utah,
USA
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 16rb Strain and binary
vector: EHAL 05 Source/ Origin (if available):
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 102c Strain and binary
vector: K1, avirulent, no Ti plasmid Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium to be imported) CFR ref. no.: Strain and binary vector:
KYRT1 Disarmed version ofstrain Chry 5 Source/ Origin (if available):
Dr. GB Collins, University of Kentucky, USA
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 15c Strain and binary
vector: LBA4404 (electro competent) Source/ Origin (if available):
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 17c Strain and binary
vector: LBA4404 (pBINmgfp5-ER) Source/ Origin (if available): Contains
green fluorescent protein. From Jim Haseloff.via R
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium collection held in containment) CFR ref. no.: Strain and
binary vector: LBA4404 (pMOG410) Source/ Origin (if available): Mogen
Internantional nv, Leiden, The Netherlands
Crop & Food Research, Palmerston North (Genetically modified
Agrobacterium collection held in containment) CFR ref. no.: Strain and
binary vector: MOG101 (pMOG410) Source/ Origin (if available): Mogen
Internantional nv, Leiden, The Netherlands
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 40cr Strain and binary
vector: pNE5 Source/ Origin (if available): Ross, CODA next to
transposase gene instead of GUS in pSLJ1111
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 44cr Strain and binary
vector: pSLJ1111 in LBA 4404 Source/ Origin (if available): From Ross)
Crop & Food Research, Lincoln (Genetically modified Agrobacterium
collection held in containment) CFR ref. no.: 31cr Strain and binary
vector: pSLJ721(EIK) in LBA 4404 Source/ Origin (if available): Plasmid
from Ross Bicknell. Promoter-Ds-GUS. Made by Eco
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Agrobacterium tumefaciens
(genetically modified)
Page 3 of 8
GMC000992
Escherichia coli (HB101
(cDNA, rbC in pBR 322)
GMC00001
GMC000993
Escherichia coli (Mc1022
(INCiII gc pJAM 252 in
PUC 18)) GMC00001
Escherichia coli (Mc1022
(INCIII gc pJAM 253 in
PUC 18)) GMC00001
Escherichia coli (pNT103
in DH5 alpha) GMC00001
GMC000994
GMC000995
GMC000996
GMC000997
GMC000998
GMC000999
Escherichia coli
(DH5alpha+ codA (5
fluoro-cytosine
susceptibility)) GMC00001
Escherichia coli ( DH5á+
Green Fluorescent Protein)
GMC00001
Escherichia coli ( DH5á+
Lc (anthocyanin regulatory
sequence leads to red
leaves)) GMC00001
Escherichia coli
((pJIT60AoPR1GUSint))
GMC00001
GMC001000
Escherichia coli (C600 (p
TOM 111 in PAC 153))
GMC00001
GMC001001
Escherichia coli (C600 (p
TOM 114 in PAC 153))
GMC00001
GMC001002
Escherichia coli (C600 (p
TOM 129 in PAC 153))
GMC00001
GMC001003
Escherichia coli (C600 (p
TOM 13 in PAC 153))
GMC00001
GMC001004
Escherichia coli (C600 (p
TOM 137 in PAC 153))
GMC00001
GMC001005
Escherichia coli (C600 (p
TOM 25 in PAC 153))
GMC00001
GMC001006
Escherichia coli (C600 (p
TOM 31 in PAC 153))
GMC00001
GMC001007
Escherichia coli (C600 (p
TOM 38 in PAC 153))
GMC00001
GMC001008
Escherichia coli (C600 (p
TOM 4 in PAC 153))
GMC00001
GMC001009
Escherichia coli (C600 (p
TOM 41 in PAC 153))
GMC00001
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
HB101 (cDNA, rbC in pBR 322 Source/ Origin (if available): Dr W.
Gruissem, Berkeley, California, USA
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (INCiII gc pJAM 252 in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (INCIII gc pJAM 253 in PUC 18) Source/ Origin (if available):
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 9cr Strain and binary vector: pNT103
in DH5 alpha Source/ Origin (if available): Originally plasmid supplied by
Nicole Houba-Herin. (Plant journal 19
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 13rb Strain and binary vector: DH5á+
codA (5 fluoro-cytosine susceptibility) Source/ Origin (if available): From
Jens Stougaard, University of Aarhus
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 2rb Strain and binary vector: DH5á+
Green Fluorescent Protein Source/ Origin (if available): From Jim
Haseloff. Oxford University, UK
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 1rb Strain and binary vector: DH5á+
Lc (anthocyanin regulatory sequence leads to red leaves) Source/ Origin
(if available): From J. Yoder. University
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 6c Strain and binary vector:
(pJIT60AoPR1GUSint) Source/ Origin (if available): From Tony Conner,
originally from Dr J. Draper, University of Leicest
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 111 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 114 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 129 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 13 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 137 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 25 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 31 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 38 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 4 in PAC 153) Source/ Origin (if available): Prof. D
Grierson University of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 41 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Page 4 of 8
GMC001010
Escherichia coli (C600 (p
TOM 5 in PAC 153))
GMC00001
GMC001011
Escherichia coli (C600 (p
TOM 6 in PAC 153))
GMC00001
GMC001012
Escherichia coli (C600 (p
TOM 66 in PAC 153))
GMC00001
GMC001013
Escherichia coli (C600 (p
TOM 75 in PAC 153))
GMC00001
GMC001014
Escherichia coli (C600 (p
TOM 88 in PAC 153))
GMC00001
GMC001015
Escherichia coli (C600 (p
TOM 92 in PAC 153))
GMC00001
GMC001016
Escherichia coli (C600 (p
TOM 94 in PAC 153))
GMC00001
GMC001017
Escherichia coli (C600 (p
TOM 96 in PAC 153))
GMC00001
GMC001018
Escherichia coli (C600 (p
TOM 99 in PAC 153))
GMC00001
GMC001019
Escherichia coli (DCMDAM) GMC00001
GMC001020
Escherichia coli (DH5a
(pEmuGN)) GMC00001
GMC001021
Escherichia coli (DH5á +
En/Spm (Maize
transposon)) GMC00001
GMC001022
Escherichia coli (DH5á +
HPT (hygromycin
resistance)) GMC00001
GMC001023
Escherichia coli
(DH5a(pAct1-D))
GMC00001
GMC001024
Escherichia coli
(DH5a(pAHC25)
GMC00001
GMC001025
Escherichia coli
(DH5a(pART8) In DH5
alpha) GMC00001
GMC001026
Escherichia coli
(DH5a(pDM302))
GMC00001
GMC001027
Escherichia coli
(DH5a(pIGI21-Hm))
GMC00001
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 5 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 6 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 66 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 75 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 88 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 92 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 94 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 96 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
C600 (p TOM 99 in PAC 153) Source/ Origin (if available): Prof. D
Grierson Univ.of Nottingham
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 34cr Strain and binary vector: DCMDAM Source/ Origin (if available): From Houba-Herin
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 5c Strain and binary vector: DH5a
(pEmuGN) Source/ Origin (if available): Professor WJ Peacock CSIRO
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 15rb Strain and binary vector: DH5á +
En/Spm (Maize transposon) Source/ Origin (if available): From Jonathon
Jones, John Innes Institute, Norwich, UK
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 6rb Strain and binary vector: DH5á +
HPT (hygromycin resistance) Source/ Origin (if available): From George
Coupland, John Innes Institute, Norwich,
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 2c Strain and binary vector:
DH5a(pAct1-D) Source/ Origin (if available): Dr Ray Wu, Cornell
University, Ithaca, NY
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 3c Strain and binary vector:
DH5a(pAHC25) Source/ Origin (if available): Professor PH Quail,
University of California, Berkley
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 1c Strain and binary vector:
DH5a(pART8) In DH5 alpha Source/ Origin (if available): Originally
plasmid supplied by Andrew Gleave (Hort Research) (Pl
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 4c Strain and binary vector:
DH5a(pDM302) Source/ Origin (if available): Dr Ray Wu, Cornell
University, Ithaca, NY
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 8c Strain and binary vector:
DH5a(pIGI21-Hm) Source/ Origin (if available): Kenzo Nakamura,
Nagoya University, Chikusa-Ku, Nagoya 464-01 Japan
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Page 5 of 8
GMC001028
Escherichia coli (DH5á+ Ac
(Maize transposon))
GMC00001
GMC001029
Escherichia coli (DH5á+
BAR (Basta resistance))
GMC00001
GMC001030
Escherichia coli (DH5á+
CAT (Chloramphenicol
resistance, an antibiotic
marker gene)) GMC00001
Escherichia coli (DH5á+
Cre/Lox recombinase )
GMC00001
GMC001031
GMC001032
Escherichia coli (DH5á+
FLP/ FRT recombinase)
GMC00001
GMC001033
Escherichia coli (DH5á+
Hieracium cDNA library)
GMC00001
Escherichia coli (DH5á+
Hieracium gDNA library)
GMC00001
Escherichia coli (DH5á+
lettuce cDNA library)
GMC00001
GMC001034
GMC001035
GMC001036
Escherichia coli (DH5á+
lettuce gDNA library)
GMC00001
GMC001037
Escherichia coli (DH5á+
Luc (Luciferase activity))
GMC00001
GMC001038
Escherichia coli
(DH5alpha+ NPTII
(Kanamycin resistance))
GMC00001
Escherichia coli (DH5á+
Spec (Spectinomycin
resistance)) GMC00001
GMC001039
GMC001040
Escherichia coli (DH5á+
SPT (Streptomycin
resistance)) GMC00001
GMC001041
Escherichia coli (DH5á+
uidA(b glucuronidase
activity)) GMC00001
GMC001042
Escherichia coli
(DH5alpha) GMC00001
GMC001043
Escherichia coli (HB101
(cDNA, GS in pUC19)
GMC00001
GMC001044
Escherichia coli (Mc 1000
(PGA 642)) GMC00001
GMC001045
Escherichia coli (Mc 1000
(PGA 580) ) GMC00001
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 14rb Strain and binary vector: DH5á+
Ac (Maize transposon) Source/ Origin (if available): From Jonathon
Jones, John Innes Institute, Norwich, UK.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 4rb Strain and binary vector: DH5á+
BAR (Basta resistance) Source/ Origin (if available): From George
Coupland, John Innes Institute, Norwich, UK.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 3rb Strain and binary vector: DH5á+
CAT (Chloramphenicol resistance, an antibiotic marker gene) Source/
Origin (if available): From Jonathon Jones, J
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 9rb Strain and binary vector: DH5á+
Cre/Lox recombinase Source/ Origin (if available): From Gary Drews,
University of Utah, US.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 10rb Strain and binary vector: DH5á+
FLP/ FRT recombinase Source/ Origin (if available): From Gary Drews,
University of Utah, US.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 16rb Strain and binary vector: DH5á+
Hieracium cDNA library Source/ Origin (if available): Made on site
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 17rb Strain and binary vector: DH5á+
Hieracium gDNA library Source/ Origin (if available): Made on site
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 18rb Strain and binary vector: DH5á+
lettuce cDNA library Source/ Origin (if available): Richard Mitchelmore,
University of California, Davis. US.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 19rb Strain and binary vector: DH5á+
lettuce gDNA library Source/ Origin (if available): Anna Koltunow,
CSIRO, Adelaide, Aus.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 8rb Strain and binary vector: DH5á+
Luc (Luciferase activity) Source/ Origin (if available): From Saco de
Vries, Wageningen Uni.The Netherlands.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 5rb Strain and binary vector: DH5á+
NPTII (Kanamycin resistance) Source/ Origin (if available):
Escherichia coli (genetically
modified)
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 12rb Strain and binary vector: DH5á+
Spec (Spectinomycin resistance) Source/ Origin (if available): From
Jonathon Jones, John Innes Institute, Norwic
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 11rb Strain and binary vector: DH5á+
SPT (Streptomycin resistance) Source/ Origin (if available): From
Jonathon Jones, John Innes Institute, Norwich,
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 7rb Strain and binary vector: DH5á+
uidA (b glucuronidase activity) Source/ Origin (if available): From
George Coupland, John Innes Institute, Norwic
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 4cr Strain and binary vector:
DH5alpha Source/ Origin (if available): From Meeghan
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
HB101 (cDNA, GS in pUC19 Source/ Origin (if available): Dr G. Coruzzi,
Rockefeller University, New York, USA
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc 1000 (PGA 642) Source/ Origin (if available): Dr G. An, Washington
State University, USA
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc 1000 (PGA 580) Source/ Origin (if available): Dr G. An, Washington
State University, USA
Escherichia coli (genetically
modified)
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Page 6 of 8
GMC001046
Escherichia coli (Mc 1000
(PGA 581)) GMC00001
GMC001047
Escherichia coli (Mc 1000
(PGA 582)) GMC00001
GMC001048
Escherichia coli (Mc 1000
(PGA 583)) GMC00001
GMC001049
Escherichia coli (Mc 1000
(PGA 643)) GMC00001
GMC001050
Escherichia coli (Mc1022
(CHI gc in PUC 18))
GMC00001
Escherichia coli (Mc1022
(CHS gc pJAM 265 in PUC
18)) GMC00001
Escherichia coli (Mc1022
(CHS gc pJAM 267 in PUC
18)) GMC00001
Escherichia coli (Mc1022
(DFR cDNA pJAM 212 in
PUC 18)) GMC00001
Escherichia coli (Mc1022
(DFR gc pAJ 18A in PUC
18)) GMC00001
Escherichia coli (Mc1022
(DFR gc pAJ 18F in PUC
18)) GMC00001
Escherichia coli (Mc1022
(F3H cDNA in pJAM 239
inPUC 18)) GMC00001
GMC001051
GMC001052
GMC001053
GMC001054
GMC001055
GMC001056
GMC001057
GMC001058
GMC001059
GMC001060
GMC001061
Escherichia coli (Mc1022
(F3H gc pJAM 262 iin PUC
18)) GMC00001
Escherichia coli (Mc1022
(INCIII cDNA pJAM 221 in
PUC 18)) GMC00001
Escherichia coli (Mc1022
(PAL cDNA in PUC 18))
GMC00001
Escherichia coli
(Mc1022CHI cDNA in PUC
18)) GMC00001
Escherichia coli (PACMUT
in DH5 alpha) GMC00001
GMC001062
Escherichia coli (pAL144)
GMC00001
GMC001063
Escherichia coli (pART7 in
DH5 alpha) GMC00001
GMC001064
Escherichia coli (pART8 in
DH5 alpha) GMC00001
GMC001065
Escherichia coli
(pBINmgfp5-ER in DH5
alpha) GMC00001
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc 1000 (PGA 581) Source/ Origin (if available): Dr G. An, Washington
State University, USA
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc 1000 (PGA 582) Source/ Origin (if available): Dr G. An, Washington
State University, USA
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc 1000 (PGA 583) Source/ Origin (if available): Dr G. An, Washington
State University, USA
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc 1000 (PGA 643) Source/ Origin (if available): Dr G. An, Washington
State University, USA
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (CHI gc in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (CHS gc pJAM 265 in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (CHS gc pJAM 267 in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (DFR cDNA pJAM 212 in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (DFR gc pAJ 18A in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (DFR gc pAJ 18F in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (F3H cDNA in pJAM 239 inPUC 18) Source/ Origin (if
available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (F3H gc pJAM 262 iin PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (INCIII cDNA pJAM 221 in PUC 18) Source/ Origin (if
available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022 (PAL cDNA in PUC 18) Source/ Origin (if available):
Crop & Food Research, Palmerston North (Genetically modified E. coli
collection held in containment) CFR ref. no.: Strain and binary vector:
Mc1022CHI cDNA in PUC 18) Source/ Origin (if available):
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 3cr Strain and binary vector:
PACMUT in DH5 alpha Source/ Origin (if available): Originally plasmid
supplied by Paszkowski,J. (Lebel et al, 1995, The
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 14cr Strain and binary vector: pAL144
Source/ Origin (if available): From J I Yoder. Contains the Lc nondestructive visual reporter gene driven by t
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 6cr Strain and binary vector: pART7
in DH5 alpha Source/ Origin (if available): Originally plasmid supplied by
Andrew Gleave (Hort Research) (Plant M
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 5cr Strain and binary vector: pART8
in DH5 alpha Source/ Origin (if available): Originally plasmid supplied by
Andrew Gleave (Hort Research) (Plant M
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 24cr Strain and binary vector:
pBINmgfp5-ER in DH5 alpha Source/ Origin (if available): Contains
green fluorescent protein. From Jim Haseloff. I elec
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Page 7 of 8
GMC001066
Escherichia coli
(PBluescript in DH5 alpha)
GMC00001
GMC001067
Escherichia coli (PDJD I
DH5 alpha) GMC00001
GMC001069
Escherichia coli (pDs- In
strain DKIP3) GMC00001
GMC001068
Escherichia coli (pDs+ In
strain DKIP3) GMC00001
GMC001070
Escherichia coli
(pEN4.MR2 in DH5α)
GMC00001
GMC001071
Escherichia coli (pHFL1 in
DH5 alpha) GMC00001
GMC001072
Escherichia coli (pIII/IV in
DH5 alpha) GMC00001
GMC001073
Escherichia coli (PKHLlacz
in DH5 alpha) GMC00001
GMC001074
Escherichia coli
(PMDSBAR in DH5 alpha)
GMC00001
GMC001075
Escherichia coli (pMiSV-H
in DH5a) GMC00001
GMC001076
Escherichia coli (pNT103
In DAM-, DCM-)
GMC00001
Escherichia coli (pNT103
In JMC5-41) GMC00001
GMC001077
GMC001078
Escherichia coli (pNT804 in
DH5 alpha) GMC00001
GMC001079
Escherichia coli (pNT806)
GMC00001
GMC001080
Escherichia coli (pSLJ1101
in DH5 alpha) GMC00001
GMC001081
Escherichia coli
(pSLJ2561) GMC00001
GMC001082
Escherichia coli
(pSLJ3621) GMC00001
GMC001083
Escherichia coli
(pVCY1638) GMC00001
GMC001084
Escherichia coli
(pVCY1660) GMC00001
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 8cr Strain and binary vector:
PBluescript in DH5 alpha Source/ Origin (if available): Stratagene
pBluescript 11 SK-. Originally I obtained the plasmi
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 2cr Strain and binary vector: PDJD I
DH5 alpha Source/ Origin (if available): Originally plasmid supplied by
Paszkowski,J. (Lebel et al, 1995, Theo A
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 22cr Strain and binary vector: pDs- In
strain DKIP3 Source/ Origin (if available): Ds inserted into Wheat Dwarf
Virus plasmid. Mutation in cis acting
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 21cr Strain and binary vector: pDs+ In
strain DKIP3 Source/ Origin (if available): Ds inserted into Wheat Dwarf
Virus plasmid. From Uwe Wirtz
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 43cr Strain and binary vector:
pEN4.MR2 in DH5á Source/ Origin (if available): Hirochika'sMaize
streak virus
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 13cr Strain and binary vector: pHFL1
in DH5 alpha Source/ Origin (if available): Hobo transposable element
from Al Handler. I electroporated into DH5
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 23cr Strain and binary vector: pIII/IV
in DH5 alpha Source/ Origin (if available): Wheat Dwarf Virus genes
required in trans for plasmid replication.
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 12cr Strain and binary vector:
PKHLlacz in DH5 alpha Source/ Origin (if available): Hobo transposase
gene. From Al Handler. I electroporated into DH5
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 1cr Strain and binary vector:
PMDSBAR in DH5 alpha Source/ Origin (if available): Originally plasmid
supplied by Paszkowski,J. (Lebel et al, 1995, Th
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 42cr Strain and binary vector: pMiSVH in DH5á Source/ Origin (if available): Hirochika's Maize streak virus
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 30cr Strain and binary vector:
pNT103 In DAM-, DCM- Source/ Origin (if available): From Houba-Herin
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 32cr Strain and binary vector:
pNT103 In JMC5-41 Source/ Origin (if available): From HoubaHerinJMC5-41 from Jack Heinemann
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 10cr Strain and binary vector:
pNT804 in DH5 alpha Source/ Origin (if available): Originally plasmid
supplied by Nicole Houba-Herin. (Plant journal 1
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 45cr Strain and binary vector:
pNT806 Source/ Origin (if available): From Houba-Herin. In DH5á
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 7cr Strain and binary vector:
pSLJ1101 in DH5 alpha Source/ Origin (if available): Originally plasmid
supplied by Steve Scofield. I electroporated
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 48cr Strain and binary vector:
pSLJ2561 Source/ Origin (if available): From Ross Bicknell
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 49cr Strain and binary vector:
pSLJ3621 Source/ Origin (if available): From Ross Bicknell
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 15cr Strain and binary vector:
pVCY1638 Source/ Origin (if available): From J I Yoder. Contains the Ac
transposase cDNA gene driven by the 35S promot
Crop & Food Research, Lincoln (Genetically modified E. coli collection
held in containment) CFR ref. no.: 16cr Strain and binary vector:
pVCY1660 Source/ Origin (if available): From J I Yoder. Contains the Lc
non-destructive visual reporter gene separated
Environmental Risk Management Authority Decision GMC00001 - Apppendix 1
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Escherichia coli (genetically
modified)
Page 8 of 8