ENVIRONMENTAL RISK MANAGEMENT AUTHORITY
DECISION
Amended under s67A on 1 October 2006
21 December 2000
Application codes
Application type
Consideration period
Considered by
GMC99008
Refer to Appendix 1 for applicant details.
Import into Containment any New Organism under section
40(1)(a) of the Hazardous Substances and New Organisms
(HSNO) Act 1996, made in accordance with section 259
for the approval of existing new microorganisms.
18 – 30 October 2000
Special Committee of the Authority appointed under
section 19(2)(b) of the HSNO Act 1996.
Decision
The application is Approved with Controls.
The organisms approved for the application are the microorganisms listed in Appendix 2 to
this decision.
Application Process
Refer to Appendix 1 for details of the application processes.
The application was not required under the Act to be publicly notified.
The documents available for the evaluation and review of the application by ERMA New
Zealand included the application and information from discussions held with the Department
of Conservation and the Ministry of Agriculture and Forestry.
As the group of applications originally considered involved both genetically modified and
non-modified organisms a special committee was established by the Authority to combine
members from both the GMO and Non-GMO Standing Committees. The Special Committee
of the Authority was appointed in accordance with section 19(2)(b) of the Hazardous
Substances and New Organisms (HSNO) Act 1996. The committee comprised members of
the Authority: Mrs Helen Hughes (Chair), Dr Lindie Nelson and Professor Barry Scott.
Relevant Legislative Criteria
The applications sought approval in accordance with section 259 of the HSNO Act 1996 to
maintain in containment, and to re-import into containment, microorganisms held as
reference specimens for reference and national and international research programmes. These
organisms were lawfully imported into New Zealand before July 1998, and the application
was made under Part V of the HSNO Act 1996, to bring previously lawful practice into line
with HSNO requirements. Section 259 applies to existing microorganisms, where no permit
was held that could be deemed to be an approval under section 241(1) or (3) of the
Transitional Provisions (Part XVI) of the HSNO Act.
The applications were lodged pursuant to section 40(1)(a) of the HSNO Act. The decisions
were determined in accordance with section 45, after taking into account the additional
matters under sections 37 and 44 of the HSNO Act, and those relevant matters under Part II
of the HSNO Act.
Consideration of the applications followed the relevant provisions of the Hazardous
Substances and New Organisms (Methodology) Order 1998, but with particular regard to
clauses 8 (dealing with the scale and significance of the risks, costs and benefits) and 26
(dealing with applications where the risks are negligible).
Reasons for the Decision
Purpose
The Committee considered that the purpose of the applications (refer to Appendix 1) was to
gain approval for existing collections of microorganisms for reference, and research
programmes. In accordance with section 45(1)(a)(i) of the Act, the Committee determined
that this was an appropriate purpose under section 39(1)(h).
Ability to Escape Containment
In accordance with section 44(b) the Committee considered the ability of the organisms to
escape from containment.
Currently the microorganisms are held in containment at the applicants’ research facilities
throughout New Zealand. The locations are either approved under MAF’s Transitional
Facilities Standard and MAF/ERMA New Zealand Standard 154.03.02: Containment
Facilities for Micro-organisms, or are in the process of gaining approval under Standard
154.03.02. The facilities will all require approval at physical containment level 1 or 2,
depending on the organisms in the collections. All the containment facilities require approval
by MAF under the MAF/ERMA Standard 154.03.02: Containment Facilities for
Microorganisms.
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In some facilities, for logistical simplicity, all the microorganisms (of both Risk Group 1 and
21 as defined in the Australian/New Zealand Standard 2243.3:20024, Safety in Laboratories:
Part 3: (Microbiology) are held at the higher level PC2 containment.
The Committee notes that these collections have been maintained for many years without
reported instances of unintended or accidental release from containment. The collections are
of pure cultures, which are used in laboratories, and are handled by researchers with
experience in microbiology. The Authority understands that most of the collections are held
ex situ on artificial media or under extremely low temperatures (e.g. minus 80°C) and cannot
simply escape or be accidentally released.
The Committee concludes that, taking into account the laboratory procedures proposed by the
applicants and the containment controls imposed in this decision, it is very unlikely that the
microorganisms could escape from the containment facility.
Establishment of self-sustaining populations and ease of eradication
In accordance with sections 37(a) and (b) of the HSNO Act the Committee considered the
ability of the organisms to establish undesirable self-sustaining populations, which might
cause adverse effects and the ease with which any such populations could be eradicated in the
event of any escape from containment.
Many of the microorganisms and GMOs are laboratory strains, that require specialised
culture regimes and/or growth factors to survive, and so have a low likelihood of survival
outside of the laboratory. Some of the organisms held in the collections may already occur
within the environment, so that any escape from containment is unlikely to have a significant
effect. Some of the microorganisms do not have their hosts in New Zealand, or their hosts are
either locally distributed or uncommon so that in the event of an escape, access of propagules
to the host and therefore establishment will both be unlikely.
Many other variables, including climate and the life stage of the organism will also determine
the ability for an escaped pathogen to establish an infection.
If microorganisms did escape and establish self-sustaining populations it is likely that only
disease causing pathogenic organisms would be detected because of their adverse effects on
host plants or animals. In this situation there would be potential for control of the pathogens
with pesticides. However, in the event of soil microorganisms establishing self-sustaining
populations it is unlikely that they would be detected and therefore little opportunity is
available to control the spread or attempted eradication. Also any control mechanism for
escaped soil organisms, which are detected, could have adverse impacts on the natural soil
microbiota.
1
Risk group 1: (low individual and community risk) Microorganisms that are unlikely to cause human, plant or animal disease (as defined in the
Australia New Zealand Standard - Safety in laboratories Part 3: Microbiology 2243:3 1995).
Risk Group 2: (moderate individual risk, limited community risk) A pathogen that can cause human, plant or animal disease, but is unlikely to be
a serious hazard to laboratory workers, the community, livestock, or the environment; laboratory exposures may cause infection, but effective
treatment and preventative measures are available, and the risk of spread is limited (as defined in the Australia New Zealand Standard - Safety in
laboratories Part 3: Microbiology 2243:3 1995).
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The Committee concludes that in the unlikely event of any self-sustaining population of
microorganisms, particularly soil microorganisms becoming established, the organisms
would be difficult to eradicate, in most cases.
The Committee notes that the applicants are required, as part of the facility registration to
maintain an internal Containment Manual, which must include contingency plans detailing
the procedures to be implemented to deal with the spillage of any microorganisms within and
outside the facility and for fire or other emergencies that may compromise containment.
Potential adverse effects
The Committee considered the potential adverse effects of the microorganisms on the
environment and public health. Any effects can only be realised should the organisms escape,
survive and establish self sustaining populations, or should laboratory staff come in contact
with harmful microorganisms.
Environment
In identifying and assessing the risks associated with these applications the applicants have
relied on the fact that these organisms are classified as Risk Group 1 and/or Risk Group 2
organisms, and therefore are unlikely to cause human, plant or animal disease (Risk Group 1)
or are unlikely to be serious hazards to laboratory workers, the community, livestock or the
environment (Risk Group 2).
Many of the organisms in these collections have been held in containment for many years
and, to the best of the applicants’ knowledge, have not had an adverse effect on the
environment or human health.
Some of the microorganisms may be able to infect native or exotic flora or fauna, and some
may occur on genera that are found in New Zealand. Consequently, in the event of an escape
from containment there is the potential for the microorganisms to establish and have an
adverse effect on native or valued exotic flora.
Public health
The Committee notes that many of the organisms in these collections have already been held
in containment for many years and, to the best of the applicants’ knowledge, have not had
adverse effects on human health. Good microbiological practice will minimise the potential
for any human pathogens included in this approval to infect laboratory workers.
Ethical Considerations
The Committee acknowledges that some of the genetically modified organisms held in these
collections contain genetic material sourced from humans. As far as could be ascertained no
human genetic material is sourced from Māori.
The applications are not for the development of GMOs but for the maintenance of existing
collections of GMOs developed either internationally or in New Zealand. The Committee
considered that any ethical approvals required for the future use and further development or
re-importation of the GMOs would be covered by the importing institutions’ ethical
guidelines and Institutional Biological Safety Committee (IBSC) delegation requirements.
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Inseparable Organisms
In accordance with section 45(1)(a)(ii) the Committee considered the effects of any
inseparable organisms. As noted above the applicants explained that all the collections are of
pure cultures.
Some applicants have noted that the possibility of inseparable organisms being present in
imported cultures could not be excluded. The Committee notes that since the organisms are
imported or held as “pure” cultures, contaminating organisms would be readily noticed in
many cases. The Committee considers that, in this application, any inseparable organisms are
very unlikely to pose risks, given that the organisms are very unlikely to escape containment
under the specified containment controls.
Negligible Risk
Based on the consideration and analysis of adverse effects to the environment and public
health, and taking into account the requirements for containment and that specialist research
staff handle the organisms, the Committee considers that the risks associated with the
maintenance and importation into containment of the microorganisms listed in Appendix 2
are negligible.
Benefits
The Committee has identified the primary benefit as being the increase in scientific
knowledge as through using the microorganisms for national and international research and
for reference purposes. The Committee is satisfied that costs are unlikely to accrue to parties
other than the applicants.
Conclusion
The microorganisms are required to be contained in facilities that are registered, operated and
managed in accordance with MAF/ERMA New Zealand Standard 154.03.02: Containment
Facilities for Microorganisms, at physical containment level PC1 for Risk Group 1 organisms
or PC2 for Risk Group 2 organisms, and the additional controls imposed in this decision.
Having considered the possible effects of the organism in accordance with sections
45(1)(a)(ii) and (iii) of the HSNO Act, the Committee is satisfied that the proposed
containment regime and additional controls can adequately contain the microorganisms.
The Committee concludes that the risks arising from maintaining and importing the
microorganisms into containment for reference and research purposes are negligible.
Therefore, the Committee has considered this application in terms of clause 26 of the
Methodology.
Taking into account the containment controls, the Committee considers that the beneficial
effects of having the organisms in containment outweigh the likely adverse effects of the
organisms and any inseparable organisms, should they escape. In this case, the fact that the
applications have been made indicates that the benefits to the applicant outweigh the costs.
The Committee is satisfied that costs are unlikely to accrue to parties other than the applicant.
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The application is approved subject the following controls in accordance with sections
45(1)(a) and 45(2) of the HSNO Act.
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Controls
1. To limit the likelihood of any accidental release of any organism or any
viable genetic material2:
1.1 The construction and operation of the various containment facilities (“the facility”)
in which the genetically modified and non-genetically modified microorganisms (as
listed in Appendix 2) are maintained and re-imported into shall be in accordance
with the:
a) Ministry of Agriculture and Forestry (MAF) Biosecurity Authority/ERMA New
Zealand Standard 154.03.023: Containment Facilities for Microorganisms.
b) Australian/New Zealand Standard AS/NZS 2243.3:20024 Safety in
Laboratories: Part 3: (Microbiological aspects and containment facilities), at
Physical Containment Level 1 (PC1) for organisms in Risk Group 15.
c) Australian/New Zealand Standard AS/NZS 2243.3:20024 Safety in
Laboratories: Part 3: (Microbiological aspects and containment facilities), at
Physical Containment Level 2 (PC2) for organisms in Risk Group 25.
1.2 The containment facilities shall be approved by MAF in accordance with the MAF
Biosecurity Authority/ERMA New Zealand Standard 154.03.023, and the controls
of the Authority.
1.3 The minimum requirement for packaging for transportation of the microorganisms
by all modes (ie. air, land and sea) from overseas and for transfers between
facilities the organism shall be according to Packaging Instruction No. 650 of the
International Air Transport Association (IATA) Dangerous Goods Regulations
(refer to MAF/ERMA New Zealand Standard 154.03.023). All containers must be
clearly labelled with the name, address and phone number of both the sender and
the recipient.
1.4 All practical and feasible measures shall be taken to ensure viable fungal material,
including spores, are contained and do not leave the containment facility through
any outlets including exhaust system, air filters and water discharge. This control
may be met by ensuring that all work with any sporulating or potentially
sporulating fungi occurs in a biological safety cabinet of Class II, in which any
water used is sterilised before disposal. Other methods may also be available to
achieve this control.
Viable Genetic Material is biological material that can be resuscitated to grow into tissues or organisms. It can be defined to
mean biological material capable of growth even though resuscitation procedures may be required, eg when organisms or parts
thereof are sublethally damaged by being frozen, dried, heated, or affected by chemical.
2
3
Or any updated Standard approved by ERMA New Zealand
4
Or any updated version endorsed by ERMA New Zealand
5
As identified in organism descriptions of Appendix 2 to this decision
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2. To exclude unauthorised people from the facility
2.1 Construction and operation of the containment facility shall comply with the
requirements contained in the standards listed in control 1.1 relating to identification
of entrances, numbers of and access to entrances, and security requirements for the
entrances and the facility.
3. To exclude other organisms from the facility and to control undesirable
and unwanted organisms within the facility
3.1 Construction and operation of the containment facility shall comply with the
standards listed in control 1.1 relating to exclusion of other organisms from the
facility and the control of undesirable and unwanted organisms within the facility.
4. To prevent unintended release of the organism by experimenters working
with the organism
4.1 Construction and operation of the containment facility shall comply with the
requirements of the standards listed in control 1.1 relating to the prevention of
unintended release of the organism by experimenters working with the organism.
5.
To control the effects of any accidental release or escape of an organism
5.1 Construction and operation of the containment facility shall comply with the
requirements contained in the standards listed in control 1.1 relating to controlling the
effects of any accidental release or escape of an organism.
5.2 If a breach of containment occurs the Facility Operator must ensure that the MAF
Inspector6 responsible for supervision of the facility has received notification of the
breach within 24 hours.
5.3 In the event of any breach of containment the contingency plan for the attempted
retrieval or destruction of any viable material of the organisms that have escaped shall
be implemented immediately. The contingency plan shall be included in the containment
manual in accordance with MAF/ERMA New Zealand Standard 154.03.023:
Containment Facilities for Microorganisms.
6.
Inspection and monitoring requirements for containment facilities
6.1 The operation of the containment facility shall comply with the requirements contained
in the standards listed in control 1.1 relating to the inspection and monitoring
requirements for containment facilities.
6.2 The Authority, or its authorised agent or properly authorised enforcement officers, may
inspect the facilities at any reasonable time.
6.3 The containment manuals shall be updated, as necessary, to address the implementation
of the controls imposed by this approval, in accordance with MAF/ERMA New Zealand
Standard 154.03.023: Containment Facilities for Microorganisms.
6
An inspector appointed under the Biosecurity Act
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7.
Qualifications required of the persons responsible for implementing those
controls
7.1 The operation of the containment facility shall comply with the requirements contained
in the standards listed in control 1.1 relating to the training of personnel working in the
facility.
7.2 The person in charge of a particular research area and/or the person in charge of the
operation of the containment facility shall inform all personnel involved in the handling
of the organisms of the Authority’s controls.
___________________
Date: 21 December 2000
Mrs Helen Hughes, Chair
Special Committee of the Authority
Amendments:
August 2006;
 to split the 19 applications from one decision to enable section 67A amendments to be
made to individual decisions
 update of the containment facility references in the controls to the most recent
MAF/ERMA technical standards and correct the Australian/New Zealand
containment facility references to “future proof” the decisions
Dr Kieran Elborough
Date: 1 October 2006
Acting Chair, New Organisms Standing Committee
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Decision Appendix 1 – Application details
1.
Application Code
Applicant
Purpose
Date Received
Date Verified
GMC99008
Fletcher Challenge Forests
Limited
To gain approval in accordance with section 259 of
the HSNO Act 1996 for the microorganisms,
Escherichia coli and Agrobacterium tumefaciens,
including modified strains, for use in vector
construction, optimisation of transformation
protocols and molecular analyses.
First received 27/07/1999
Resubmitted 07/01/2000
Resubmitted 23/06/2000
07/07/2000
Contacts:
Chris Jones, Rebecca Jones
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Decision Appendix 2 –List of Approved Organisms
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Application:
GMC99008
Approval
Code
GMC000202
Application
Code
GMC99008
A. rhizogenes A4T
Agrobacterium
tumefaciens
GMC000203
GMC99008
A. rhizogenes pB162
Agrobacterium
rhizogenes
GMC000204
GMC99008
A. rhizogenes pBI 121
Agrobacterium
rhizogenes
GMC000205
GMC99008
A. rhizogenes
pBINmgfp5ER
Agrobacterium
rhizogenes
GMC000206
GMC99008
A. rhizogenes pLM9
Agrobacterium
rhizogenes
GMC000207
GMC99008
A. tumefaciens 35SGUSINT
Agrobacterium
tumefaciens
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Common Name
Taxonomic Name
Organism Description
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium rhizogenes (Risk Group 1)
Code: Agro 3
Strain: A4T
Description:
DNA:
Promoters:
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 13
Strain: A4T
Description: Virulence construct used to test the virulence of
Agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression (Otten L, Salomone JY, Helfer A, Schmidt J,
Hammann P, De Ruffray P Sequence and functional analysis of
the left-hand part of the T-region from the nopaline-type Ti
plasmid, pTiC58. Plant Mol Biol 1999 41, 765-76.)
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Chloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 14
Strain: A4T
Description: Purchased from Clonetech, a standard binary vector
containing restriction sites
DNA: Gus, Npt II
Promoters: Nos, CaMV-35S
Selection: Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 15
Strain: A4T
Description: Contains Green Fluorescent Protein (GFP) gene, as
an alternative reporter gene (Haseloff et al., 1997)
DNA: GFP, Npt II
Promoters: Nos, CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 12
Strain: A4T
Description: Contains matrix attachment regions, which has
shown to stabilises the integration of foreign DNA into the plant
genome
DNA: Gus, Npt II, Nos and matrix attachment regions
Promoters: CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 23
Strain: A281
Description: b-glucuronidase gene with plant specific intron, the
gene would only be expressed by plant cells. Also contains an
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000208
GMC99008
A. tumefaciens 6025
Agrobacterium
tumefaciens
GMC000209
GMC99008
A. tumefaciens A281
Agrobacterium
tumefaciens
GMC000210
GMC99008
A. tumefaciens A281/pB162 Agrobacterium
tumefaciens
GMC000211
GMC99008
A. tumefaciens
A281/pBINmgfp5ER
GMC000212
GMC99008
A. tumefaciens A281/pKIWI Agrobacterium
105
tumefaciens
GMC000213
GMC99008
A. tumefaciens A281/pKIWI Agrobacterium
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Agrobacterium
tumefaciens
Organism Description
gene resistant to a group of Aminoglycoside antibiotics.
DNA: Gus, Npt II
Promoters: Nos, CaMV-35S
Selection: Kanamycin
Backbone of the construct: pBIN19 (Vancanneyt et al., 1990)
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 9
Strain: 6025
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 1
Strain: A281
Description: Hood E.E., Helmer G.L., Fraley R.T., Chilton M.-D.
(1986) The hypervirulence of Agrobacterium tumefaciens strain
A281 is encoded in a region of pTiBo542 outside of the T-DNA. J.
Bacteriol. 168:1291-1301
DNA:
Promoters:
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 28
Strain: A281
Description: Virulence construct used to test the virulence of
Agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Uid A, Npt II
Promoters: Nos
Selection: Rifampicin Tetracycline Chloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 29
Strain: A281
Description: Contains Green Fluorescent Protein (GFP) gene, as
an alternative reporter gene
DNA: GFP, Npt II
Promoters: Nos, CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 31
Strain: A281
Description: Basic reporter genes to confirm transformation event
DNA: Uid A, Npt II
Promoters: CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1/06/98
Fletcher Challenge Forests, Te Teko Collection of genetically
Approval
Code
Application
Code
Common Name
Taxonomic Name
1052
tumefaciens
GMC000214
GMC99008
A. tumefaciens A6
Agrobacterium
tumefaciens
GMC000215
GMC99008
A. tumefaciens A722
Agrobacterium
tumefaciens
GMC000216
GMC99008
A. tumefaciens AGL1
Agrobacterium
tumefaciens
GMC000217
GMC99008
A. tumefaciens AGL1/pB162 Agrobacterium
tumefaciens
GMC000218
GMC99008
A. tumefaciens
AGL1/pBINmgfp5ER
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Agrobacterium
tumefaciens
Organism Description
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 30
Strain: A281
Description: Same construct as pKIWI 105, containing basic
reporter genes except a double CaMV 35S promoter drives Gus
reporter gene
DNA: Uid A, Npt II
Promoters: Double CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1/06/98
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 8
Strain: A6
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 7
Strain: A722
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 10
Strain: AGL1
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 24
Strain: AGL1
Description: Virulence construct used to test the virulence of
Agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Chloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 26
Strain: AGL1
Description: Contains Green Fluorescent Protein (GFP) gene, as
an alternative report gene
DNA: GFP, Npt II
Promoters: Nos, CaMV-35S
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000219
GMC99008
A. tumefaciens AGL1/pKIWI Agrobacterium
105
tumefaciens
GMC000220
GMC99008
A. tumefaciens AGL1/pKIWI Agrobacterium
1052
tumefaciens
GMC000221
GMC99008
A. tumefaciens B6
Agrobacterium
tumefaciens
GMC000222
GMC99008
A. tumefaciens B653
Agrobacterium
tumefaciens
GMC000223
GMC99008
A. tumefaciens C58
Agrobacterium
tumefaciens
GMC000224
GMC99008
A. tumefaciens C58/pB162 Agrobacterium
tumefaciens
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Organism Description
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 27
Strain: AGL1
Description: Basic reporter genes to confirm transformation event
(Janssen et al., 1989)
DNA: Uid A, Npt II
Promoters: CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1/06/98
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 25
Strain: AGL1
Description: Same construct as pKIWI 105, containing basic
reporter genes except a double CaMV 35S promoter drives Gus
reporter gene
DNA: Uid A, Npt II
Promoters: Double CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1/06/98
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 5
Strain: B6
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 6
Strain: B653
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 4
Strain: C58
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 18
Strain: C58
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000225
GMC99008
A. tumefaciens
C58/pBINmgfp5ER
Agrobacterium
tumefaciens
GMC000226
GMC99008
A. tumefaciens C58/pKIWI
1052
Agrobacterium
tumefaciens
GMC000227
GMC99008
A. tumefaciens EHA 105
Agrobacterium
tumefaciens
GMC000228
GMC99008
A. tumefaciens EHA
105/pKIWI 1052
Agrobacterium
tumefaciens
GMC000229
GMC99008
A. tumefaciens EHA101
Agrobacterium
tumefaciens
GMC000230
GMC99008
A. tumefaciens
EHA101/pB162
Agrobacterium
tumefaciens
D:\612939123.doc
Organism Description
Description: Virulence construct used to test the virulence of
Agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Chloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 19
Strain: C58
Description: Contains Green Fluorescent Protein (GFP) gene, as
an alternative reporter gene
DNA: GFP, Npt II
Promoters: Nos, CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1) Code: Agro
17 Strain: C58 Description: Same construct as pKIWI 105,
containing basic reporter genes except a double CaMV 35S
promoter drives Gus reporter gene DNA: Uid A, Npt II Promoters:
Double CaMV-35S, Nos Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector Location: -70C freezer, PC2 Supplier: Genesis; Dr. Barry
Flinn Received: 1/06/98
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 38
Strain: EHA 105
Description:
DNA:
Promoters:
Selection:
Backbone of the construct:
Location: -70C freezer- isolated box 4, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 39
Strain: EHA 105
Description: Same construct as pKIWI 105, containing basic
reporter genes except a double CaMV 35S promoter drives Gus
reporter gene
DNA: Uid A, Npt II
Promoters: Double CaMV-35S, Nos
Selection: Kanamycin
Backbone of the construct: Binary vector, pBIN
Location: -70C freezer- isolated box 4, PC2
Supplier: Hort+Research; Kim Richardson; Genesis; Dr. Barry
Flinn
Received: 29/01/2000; 1/06/1998
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 2
Strain: EHA101
Description: Hood E.E., Helmer G.L., Fraley R.T., Chilton M.-D.
(1986) The hypervirulence of Agrobacterium tumefaciens strain
A281 is encoded in a region of pTiBo542 outside of the T-DNA. J.
Bacteriol. 168:1291-1301
DNA:
Promoters:
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000231
GMC99008
A. tumefaciens GV3101
Agrobacterium
tumefaciens
GMC000232
GMC99008
A. tumefaciens LBA
4301/pB162
Agrobacterium
tumefaciens
GMC000233
GMC99008
A. tumefaciens LBA
4404/pB162
Agrobacterium
tumefaciens
GMC000234
GMC99008
A. tumefaciens pAX4cL
Agrobacterium
tumefaciens
GMC000235
GMC99008
A. tumefaciens pAX6
Agrobacterium
tumefaciens
D:\612939123.doc
Organism Description
Code: Agro 22
Strain: EHA101
Description: Virulence construct used to test the virulence of
Agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Chloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 11
Strain: GV3101
Description:
DNA:
Promoters:
Selection
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 20
Strain: LBA 4301
Description: Virulence construct used to test the virulence of
Agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Chloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 21
Strain: LBA 4404
Description: Virulence construct used to test the virulence of
Agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Chloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 32
Strain: AGL1
Description: UDPG-pase gene isolated from Acetobacter xylinum,
driven by a promoter from parsley in the binary vector pBI121
DNA: Uid A, Npt II
Promoters: 4cl, Nos
Selection: Kanamycin
Backbone of the construct: Binary vector pBI120
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 33
Strain: AGL1
Description: UDPG-pase gene isolated from Acetobacter xylinum,
driven by a CaMV35S promoter in the binary vector pBI121
DNA: UDPG-pase, Npt II
Promoters: CaMV-35S, Nos
Selection: Kanamycin
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000236
GMC99008
A. tumefaciens pLM9
Agrobacterium
tumefaciens
GMC000237
GMC99008
E. coli pCW2000
Escherichia coli
GMC000238
GMC99008
E. coli pRK 2013
Escherichia coli
GMC000239
GMC99008
E. coli Bluescript DH5a/IISK+
Escherichia coli
GMC000240
GMC99008
E. coli Bluescript XL1
Blue/II-SK+
Escherichia coli
GMC000241
GMC99008
E. coli DH5a/pB162
Escherichia coli
GMC000242
GMC99008
E. coli DH5a/pBI-121
Escherichia coli
D:\612939123.doc
Organism Description
Backbone of the construct: Binary vector pBI121
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Agrobacterium tumefaciens (Risk Group 1)
Code: Agro 16
Strain: C58
Description: Contains matrix attachment regions, which have
shown to stabilise the integration of foreign DNA into the plant
genome (Nap et al., 1997)
DNA: Gus, Npt II, Nos and matrix attachment regions
Promoters: CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 20
Strain: DH5a
Description: Contains Green Fluorescent Protein (GFP) gene
driven by a maize ubiquitin promoter
DNA: GFP, amp
Promoters: Uid A
Selection: Ampicillin
Backbone of the construct: pJIT 166
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 9
Strain: HB101
Description:
DNA:
Promoters:
Selection: Kanamycin
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 4
Strain: DH5a
Description: Multi purpose construct for cloning
DNA: LacZ, amp, MCS
Promoters: NA
Selection: Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 5
Strain: XL1 Blue
Description: Multi purpose construct for cloning
DNA: LacZ, amp, MCS
Promoters: NA
Selection: Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 25
Strain: DH5a
Description: Virulence construct used to test the virulence of
agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Cloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000243
GMC99008
E. coli DH5a/pBINmgfp5ER Escherichia coli
GMC000244
GMC99008
E. coli DH5a/pBinSyn-gus
Escherichia coli
GMC000245
GMC99008
E. coli DH5a/pCW122
Escherichia coli
GMC000246
GMC99008
E. coli DH5a/pKIWI 105
Escherichia coli
GMC000247
GMC99008
E. coli DH5a/pKIWI 1052
Escherichia coli
GMC000248
GMC99008
E. coli DH5a/pLM9
Escherichia coli
D:\612939123.doc
Organism Description
Code: EC 27
Strain: DH5a
Description: Purchased from Clonetech, a standard binary vector
containing enzyme restriction sites
DNA: Uid A, Npt II
Promoters: Nos
Selection: Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 12
Strain: DH5a
Description: Contains Green Fluorescent Protein (GFP) gene, as
an alternative reporter gene
DNA: GFP, Npt II
Promoters: Nos, CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 46
Strain: DH5a
Description: Binary vector containing a Kanamycin resistant gene
driven by a nos promoter, with a b-glucuronidase gene driven by
35S-AMV promoter
DNA: Npt II, Uid A
Promoters: CaMV-35S, Nos, AMV
Selection: Kanamycin
Backbone of the construct: Binary Vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 66
Strain: DH5a
Description: 2 x 35S - uid A; 35S - npt II
DNA: Uid A; Npt II
Promoters: CaMV 35S, double CaMV 35S
Selection:
Backbone of the construct: k=kozak sequence, pUC-derived
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 28
Strain: DH5a
Description: Basic reporter genes to confirm transformation event
DNA: Uid A, Npt II
Promoters: CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 36
Strain: DH5a
Description: Same construct as pKIWI 105, containing basic
reporter genes except a double CaMV 35S promoter drives Gus
reporter gene
DNA: Uid A, Npt II
Promoters: Double CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1/06/98
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 13
Strain: DH5a
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000249
GMC99008
E. coli DH5alpha
Escherichia coli
GMC000250
GMC99008
E. coli HB101
Escherichia coli
GMC000251
GMC99008
E. coli HB101/pB162
Escherichia coli
GMC000252
GMC99008
E. coli HB101/pBI-121
Escherichia coli
GMC000253
GMC99008
E. coli
HB101/pBINmgfp5ER
Escherichia coli
GMC000254
GMC99008
E. coli HB101/pBinSyn-g
Escherichia coli
D:\612939123.doc
Organism Description
Description: Contains matrix attachment regions, which have
shown to stabilise the integration of foreign DNA into the plant
genome
DNA: Uid A, Npt II, Nos and matrix attachment regions
Promoters: CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 1
Strain: DH5alpha
Description: Multi purpose construct for cloning in vectors using
alpha-complementation for blue/white screening.
DNA: LacZ, amp, MCS
Promoters: NA
Selection: Ampicillin
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 2
Strain: HB101
Description: Multi purpose construct for cloning in vectors that do
not require alpha-complementation for blue/white screening.
DNA: LacZ, amp, MCS
Promoters: NA
Selection: Ampicillin
Backbone of the construct:
Location: -70C freezer, PC2
Supplier:
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 8
Strain: HB101
Description: Virulence construct used to test the virulence of
agrobacterium strains on plant. Onc region is from pTiC58 for
plant expression
DNA: Npt II, Nos, Onc
Promoters: Nos
Selection: Rifampicin Tetracycline Cloramphenicol
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 26
Strain: HB101
Description: Purchased from Clonetech, a standard binary vector
containing enzyme restriction sites
DNA: Uid A, Npt II
Promoters: Nos
Selection: Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 11
Strain: HB101
Description: Contains Green Fluorescent Protein (GFP) gene, as
an alternative reporter gene
DNA: GFP, Npt II
Promoters: Nos, CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000255
GMC99008
E. coli HB101/pKIWI 105
Escherichia coli
GMC000256
GMC99008
E. coli HB101/pKIWI 1052
Escherichia coli
GMC000257
GMC99008
E. coli HB101/pLM9
Escherichia coli
GMC000258
GMC99008
E. coli p BI 101
Escherichia coli
GMC000259
GMC99008
E. coli p KDK
Escherichia coli
GMC000260
GMC99008
E. coli p KEGN
Escherichia coli
D:\612939123.doc
Organism Description
Code: EC 45
Strain: HB101
Description: Binary vector containing a Kanamycin resistant gene
driven by a nos promoter, with a b-glucuronidase gene driven by
35S-AMV promoter
DNA: Npt II, Uid A
Promoters: CaMV-35S, Nos, AMV
Selection: Kanamycin
Backbone of the construct: Binary Vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 29
Strain: HB101
Description: Basic reporter genes to confirm transformation event
DNA: Uid A, Npt II
Promoters: CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 37
Strain: HB101
Description: Same construct as pKIWI 105, containing basic
reporter genes except a double CaMV 35S promoter drives Gus
reporter gene
DNA: Uid A, Npt II
Promoters: Double CaMV-35S, Nos
Selection: Rifampicin Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 1/06/98
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 10
Strain: HB101
Description: Contains matrix attachment regions, which have
shown to stabilise the integration of foreign DNA into the plant
genome
DNA: Uid A, Npt II, Nos and matrix attachment regions
Promoters: CaMV-35S
Selection: Rifampicin Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 58
Strain: DH5a
Description: Purchased from Clonetech, a standard binary vector
containing enzyme restriction sites
DNA: Uid A, Npt II
Promoters: Nos
Selection: Kanamycin
Backbone of the construct: Binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 56
Strain: DH5a
Description: b-glucuronidase and Pinus radiata gene that only
expresses in the male and female strobili
DNA: Uid A, Npt II, PrAG1-DK
Promoters: CaMV 35S, double CaMV 35S, Nos, AMV
Selection: Kanamycin, Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000261
GMC99008
E. coli p2707
Escherichia coli
GMC000262
GMC99008
E. coli p35s-Gus-int
Escherichia coli
GMC000263
GMC99008
E. coli pAGE 1
Escherichia coli
GMC000264
GMC99008
E. coli pAGE 2
Escherichia coli
GMC000265
GMC99008
E. coli pASC6
Escherichia coli
GMC000266
GMC99008
E. coli pBI-asc6s
Escherichia coli
D:\612939123.doc
Organism Description
Code: EC 55
Strain: DH5a
Description: b-glucuronidase gene
DNA: Uid A, Npt II, amp
Promoters: double CaMV 35S, AMV
Selection: Kanamycin, Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 41
Strain: DH5a
Description:
DNA:
Promoters:
Selection: Ampicillin
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 48
Strain: DH5a
Description: b-glucuronidase gene with plant specific intron, the
gene would only be expressed by plant cells. Also contains an
gene resistant to a group of Aminoglycoside antibiotics.
DNA: Uid A, Npt II
Promoters: CaMV 35S
Selection: Kanamycin
Backbone of the construct: pBIN19
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 105
Strain: DH5a
Description: Eucalyptus Agamous homologue
DNA: Amp
Promoters: T7 promoter
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 106
Strain: DH5a
Description: Eucalyptus Agamous homologue (antisense)
DNA: Amp
Promoters: T7 promoter
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 38
Strain: DH5a
Description: The aspen ferulic acid 5-hydroxylase gene was
isolated from an aspen xylem cDNA library and cloned into
Bluescript.
DNA: asc6, LacZ, MCS, amp,
Promoters: NA
Selection: Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 40
Strain: TOP 10F
Description: The aspen ferulic acid 5-hydroxylase gene was then
cloned into binary vector pBI121 in the sense orientation
DNA: Npt II, asc6
Promoters: CaMV-35S, Nos
Selection: Kanamycin
Backbone of the construct: pBI121, Binary Vector
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000267
GMC99008
E. coli pBK-CMV (Actin)
Escherichia coli
GMC000268
GMC99008
E. coli pcPI -7Z
Escherichia coli
GMC000269
GMC99008
E. coli pCW122
Escherichia coli
GMC000270
GMC99008
E. coli pCW2000
Escherichia coli
GMC000271
GMC99008
E. coli pCW2000 D GFP
Escherichia coli
GMC000272
GMC99008
E. coli pCW5
Escherichia coli
GMC000273
GMC99008
E. coli pDW143:1
Escherichia coli
D:\612939123.doc
Organism Description
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 14
Strain: XL1 Blue
Description: Actin homologue from Pinus radiata from early cDNA
library
DNA: PraActin, Npt II
Promoters: NA
Selection: Kanamycin
Backbone of the construct: pLNB293
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Leonard Bloksberg
Received: 1997
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 7
Strain: DH5a
Description: Leafy/Agamous homologue clone from a cDNA
library into Promega cloning vector pGEM-7Zf
DNA: Leafy genomic
Promoters: NA
Selection: Ampicillin
Backbone of the construct: pGEM-7Zf
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 19
Strain: DH5a
Description: b-glucuronidase gene with a Kozak sequence
between the double CaMV35S promoter,
DNA: Uid A, Npt II, amp
Promoters: CaMV 35S, double CaMV 35S
Selection: Kanamycin
Backbone of the construct: pJIT 166
Location: -70C freezer, PC2
Supplier: Forest Research Institute, Dr Christian Walter (Geenz)
Received: 199
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 86
Strain: DH5a
Description: GFP
DNA: GFP
Promoters:
Selection:
Backbone of the construct: pUC-derived
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 21
Strain: DH5a
Description: pCW2000 with the GFP gene deleted from the
construct
DNA: amp
Promoters: Uid A
Selection: Ampicillin
Backbone of the construct: pJIT 167
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 71
Strain: DH5a
Description: 35S - uid A
DNA: Uid A
Promoters: CaMV 35S
Selection:
Backbone of the construct: pUC-derived
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000274
GMC99008
E. coli pELF2A
Escherichia coli
GMC000275
GMC99008
E. coli pEND4K
Escherichia coli
GMC000276
GMC99008
E. coli pF730
Escherichia coli
GMC000277
GMC99008
E. coli pFCF001
Escherichia coli
GMC000278
GMC99008
E. coli pFCF002
Escherichia coli
GMC000279
GMC99008
E. coli pFCF003 (Bluescript Escherichia coli
D HindIII)
D:\612939123.doc
Organism Description
Code: EC 6
Strain: DH5a
Description: Leafy homologue PCR from Gingko biloba and
cloned into Bluescript
DNA: LacZ, amp, MCS,Leafy genomic
Promoters: NA
Selection: Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 109
Strain: DH5a
Description: elf antisense
DNA: Amp
Promoters: T7 promoter
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 47
Strain: DH5a
Description: Binary vector containing a Kanamycin resistant gene
driven by a nos promoter, with a few restriction enzyme sites
DNA: Npt II
Promoters: Nos
Selection: Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 3
Strain: DH5a
Description: Leafy homologue (AP3) from Arabidopsis and cloned
into pGem3Z vector
DNA: AP3
Promoters: NA
Selection: Ampicillin
Backbone of the construct: pGEM3Z
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 30
Strain: DH5a
Description: Green Fluorescent Protein gene in Bluescript
DNA: Amp, MCS
Promoters: NA
Selection: Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 31
Strain: DH5a
Description: Green Fluorescent Protein gene in Bluescript
DNA: Amp, MCS
Promoters: NA
Selection: Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 32
Strain: DH5a
Description: Standard off the shelf Bluescript plasmid with the
restrictive enzyme site Hind III deleted from the multi-cloning site
DNA: LacZ, MCS, amp,
Promoters: NA
Selection: Ampicillin
Backbone of the construct: pBluescript
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000280
GMC99008
E. coli pFCF004 (Bluescript Escherichia coli
D EcoR1)
GMC000281
GMC99008
E. coli pFCF005
Escherichia coli
GMC000282
GMC99008
E. coli pFCF006
Escherichia coli
GMC000283
GMC99008
E. coli pFOMT 1
Escherichia coli
GMC000284
GMC99008
E. coli pGus4cL
Escherichia coli
GMC000285
GMC99008
E. coli pHAGPR
Escherichia coli
D:\612939123.doc
Organism Description
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 33
Strain: DH5a
Description: Standard off the shelf Bluescript plasmid with the
restrictive enzyme site EcoRI deleted from the multi-cloning site
DNA: LacZ, MCS, amp,
Promoters: NA
Selection: Ampicillin
Backbone of the construct: pBluescript
Location: -70C freezer, PC2
Supplier: Fletcher Challenge Forest; Dr. Kumar Sivakumaran
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 34
Strain: DH5a
Description: Removal of all genes between the left and right
border of pEND4K (the back bone with left and right borders)
DNA: Npt I
Promoters: Nos
Selection: Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Supplier: Fletcher Challenge Forest; Dr. Kumar Sivakumaran
Received:
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 35
Strain: DH5a
Description: A multi purpose binary cloning vector with a multiple
cloning site.
DNA: Npt I, MCS
Promoters: Nos
Selection: Kanamycin
Backbone of the construct: pEND4K (Klee et al., 1985) Binary
vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 44
Strain: DH5a
Description: The Aspen OMT gene was cloned from xylem
specific library into Binary vector for transformation
DNA: Npt II, Aspen OMT
Promoters: CaMV-35S, Nos, AMV
Selection: Kanamycin
Backbone of the construct: Binary Vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 24
Strain: DH5a
Description: b-glucuronidase gene with promoter from parsley in a
PUC vector
DNA: Uid A, Npt II
Promoters: 4cl, Nos
Selection: Kanamycin
Backbone of the construct: PUC 18
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 51
Strain: DH5a
Description: RNase cytotoxic gene fused to Pinus radiata
promoter that only expresses in the male and female flowers
DNA: HPT, RNS2
Promoters: PrAG-Pro, CaMV 35S
Selection: Kanamycin
Backbone of the construct: pGEM-T easy vector
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000286
GMC99008
E. coli pID 11
Escherichia coli
GMC000287
GMC99008
E. coli pKDC
Escherichia coli
GMC000288
GMC99008
E. coli pKEH
Escherichia coli
GMC000289
GMC99008
E. coli pPMB 706
Escherichia coli
GMC000290
GMC99008
E. coli pPMB 7088
Escherichia coli
GMC000291
GMC99008
E. coli pPMB 765
Escherichia coli
GMC000292
GMC99008
E. coli pPMB 768
Escherichia coli
D:\612939123.doc
Organism Description
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 42
Strain: DH5a
Description:
DNA:
Promoters:
Selection: Ampicillin
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 54
Strain: DH5a
Description: b-glucuronidase and Pinus radiata gene that only
expresses in the male and female strobili
DNA: Uid A, Npt II, PrAG1-DK, amp
Promoters: CaMV 35S, double CaMV 35S, Nos, AMV
Selection: Kanamycin, Ampicillin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 53
Strain: DH5a
Description: Hygromycin resistant gene fused to CaMV 35S gene
DNA: HPT
Promoters: CaMV 35S
Selection: Ampicillin Hygromycin
Backbone of the construct: Bluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 16
Strain: DH5a
Description: Metalloregulatory element fused with CaMV 35s
promoter
DNA: MRE
Promoters: CaMV-35S
Selection: Ampicillin
Backbone of the construct: PUC 120
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 18
Strain: DH5a
Description: Binary vector containing metalloregulatory element
fused with CaMV 35s promoter, gives expression of the induced
gene in all plant organs under copper inducing conditions
DNA: ace1, Npt II
Promoters: CaMV-35S, Nos
Selection: Kanamycin
Backbone of the construct: Binary vector pART (Gleave et
al.,1992)
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 17
Strain: DH5a
Description: Binary vector containing ace1 gene fused with CaMV
35s promoter, gives expression of the induced gene in all plant
organs under copper inducing conditions
DNA: ace1, Npt II
Promoters: CaMV-35S, Nos
Selection: Kanamycin
Backbone of the construct: Binary vector pART (Gleave et
al.,1992)
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000293
GMC99008
E. coli pPtomT/SK
Escherichia coli
GMC000294
GMC99008
E. coli pRAD 0446f
Escherichia coli
GMC000295
GMC99008
E. coli pRAD 0819b
Escherichia coli
GMC000296
GMC99008
E. coli pRAGPR
Escherichia coli
GMC000297
GMC99008
E. coli pRC101
Escherichia coli
GMC000298
GMC99008
E. coli pSP 72 CADpro
1B+2B
Escherichia coli
D:\612939123.doc
Organism Description
Code: EC 15
Strain: DH5a
Description: Metalloregulatory element fused with CaMV 35s
promoter
DNA: MRE
Promoters: CaMV-35S
Selection: Ampicillin
Backbone of the construct: PUC 119
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 43
Strain: DH5a
Description: The full length Aspen OMT gene was cloned from
xylem specific library into Bluescript
DNA: LacZ, MCS, amp, Aspen OMT
Promoters: NA
Selection: Ampicillin
Backbone of the construct: pBluescript
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 101
Strain: XL-1 Blue
Description: Pine 4CL antisense
DNA: 4CL, npt11
Promoters: 35S
Selection:
Backbone of the construct: pART27
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 21/10/99
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 103
Strain: XL-1 Blue
Description: Pine UDPG-Pase sense
DNA: npt11
Promoters: 35S
Selection:
Backbone of the construct: pART27
Location: -70C freezer, PC2
Supplier: Genesis; Dr. Barry Flinn
Received: 21/10/99
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 52
Strain: DH5a
Description: RNase cytotoxic gene fused to Pinus radiata
promoter that only expresses in the male and female flowers
DNA: HPT, RNS3
Promoters: PrAG-Pro, CaMV 35S
Selection: Kanamycin
Backbone of the construct: Binary vector, pRD420
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 79
Strain: DH5a
Description: p40CS - uid A; 35S npt II
DNA: uid A, Npt II
Promoters: p40CS
Selection:
Backbone of the construct: binary vector
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 39
Strain: TOP 10
Description: The CAD promoter from Pinus radiata was isolated
and cloned into clonetech vector pSP72
DNA: CAD2B + CAD1B (CAD promoter), amp
Approval
Code
Application
Code
Common Name
Taxonomic Name
GMC000299
GMC99008
E. coli pSQE 1
Escherichia coli
GMC000300
GMC99008
E. coli pSQE 2
Escherichia coli
GMC000301
GMC99008
E. coli pTAG
Escherichia coli
GMC000302
GMC99008
E. coli pTDC
Escherichia coli
GMC000303
GMC99008
E. coli pTDK
Escherichia coli
D:\612939123.doc
Organism Description
Promoters: CAD
Selection: Ampicillin
Backbone of the construct: pSP72
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 107
Strain: DH5a
Description:
DNA: nptII
Promoters: 35S
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 108
Strain: DH5a
Description:
DNA: nptII
Promoters: 35S
Selection:
Backbone of the construct:
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 57
Strain: DH5a
Description: b-glucuronidase and Pinus radiata gene that only
expresses in the male and female strobili
DNA: Uid A, Npt II, PrAG1-DK
Promoters: CaMV 35S, double CaMV 35S, Nos, AMV
Selection: Kanamycin, Ampicillin
Backbone of the construct: Binary Vector BIN
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 49
Strain: DH5a
Description: b-glucuronidase and Pinus radiata gene that only
expresses in the male and female strobili
DNA: Uid A, Npt II, PrAG1-DC
Promoters: CaMV 35S, double CaMV 35S, Nos
Selection: Kanamycin
Backbone of the construct: Binary vector, pBIN
Location: -70C freezer, PC2
Fletcher Challenge Forests, Te Teko Collection of genetically
modified Escherichia coli (Risk Group 1)
Code: EC 50
Strain: DH5a
Description: b-glucuronidase and Pinus radiata gene that only
expresses in the male and female strobili
DNA: Uid A, Npt II, PrAG1-DK
Promoters: CaMV 35S, double CaMV 35S, Nos
Selection: Kanamycin
Backbone of the construct: Binary vector, pBIN
Location: -70C freezer, PC2