Proteomics Class, Summer, 2001
M. Lawrence Clevenson
Edward J. Carroll

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1. To expose students to a wellreplicated proteomic (gel) data set
2. To lead students to explore criteria for comparing models for this data set
3. To guide students working in groups to prepare a presentation of their analysis and results

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Hands-On Activity with Computers for
About Two Hours after Lunch
Afternoon Computer Activities Amplified
Morning Lectures
Elementary Statistical and Graphing
Examples—Model Fitting, Residuals
Early Exposure to Proteomic Data

Gas Used vs Heaing Degree Days
12
10
8
6
4
2
0
0 y = 0.189x + 1.0892
R
2
= 0.9906
10
Average Gas (100 ft^3) per day
Predicted Gas Usage from Linear Model
Linear (Average Gas (100 ft^3) per day)
20 30
Average Degree Days
40 50 60

Fuel used (liters/100 km)
25
20
15
10
5
0
0 20 40 60 80
Spe e d in km/hr
100 120 140 160

GE L ID cm Interface-D ye Front cm band 1 cm band 2 cm band 3 cm band 4 cm band 5 cm band 6 cm band 7 cm band 8 cm band 9
3-28-01 # 1
3-28-01 # 2
3-28-01-# 3
4-4-01 # 1
4-4-01 # 2
4-4-01 # 3
4-4-01 # 4
4-4-01 # 5
4-l7-01 # 1
4-17-01 # 2
4-17-01 # 3
4-17-01 # 4
4-18-01 # 1
4-18-01 # 2
4-18-01 # 3
4-18-01 # 4
4-18-01 # 5
4-18-01 # 6
4-18-01 # 7
4-18-01 # 8
6.90
6.90
6.90
7.00
7.00
7.15
7.00
7.15
6.35
6.35
6.35
6.35
6.20
6.20
6.20
6.20
6.20
6.20
6.20
6.20
1.10
0.90
0.85
0.85
0.80
0.90
0.90
0.80
1.10
0.90
1.05
0.70
0.80
0.80
0.80
0.90
1.05
1.05
0.80
0.80
1.85
1.75
1.65
1.65
1.50
1.75
1.85
1.50
1.85
1.70
1.85
1.55
1.60
1.55
1.55
1.70
1.80
1.85
1.55
1.50
2.65
2.50
2.50
2.55
2.45
2.60
2.70
2.40
2.55
2.35
2.50
2.35
2.35
2.35
2.25
2.35
2.45
2.55
2.25
2.15
2.15
2.00
1.95
1.95
1.85
2.05
2.15
1.85
2.10
1.95
2.10
1.85
1.85
1.85
1.85
1.95
2.05
2.10
1.80
1.80
3.35
3.20
3.30
3.30
3.25
3.50
3.60
3.30
3.20
3.05
3.15
3.10
3.05
3.05
2.95
3.00
3.05
3.20
3.00
2.85
4.20
4.05
4.15
4.25
4.20
4.45
4.50
4.20
3.90
3.80
3.80
3.85
3.80
3.80
3.65
3.65
3.75
3.85
3.65
3.55
4.90
4.80
4.90
5.10
5.10
5.45
5.40
5.05
4.50
4.45
4.25
4.55
4.35
4.35
4.25
4.25
4.35
4.40
4.25
4.15
5.30
5.30
5.35
5.60
5.60
6.10
5.90
5.50
4.95
4.90
4.75
5.00
4.75
4.75
4.60
4.60
4.75
4.75
4.65
4.50
5.60
5.60
5.60
5.90
5.95
6.35
6.20
5.75
5.20
5.20
5.05
5.30
5.00
5.00
4.85
4.80
5.05
5.00
4.90
4.75
Protein Bio-Rad Given Mol Wt Log
(daltons) Mol Wt
Myosin 200,000 5.30
beta-Galactosidase 116,250 5.07
Phosphorylase B 97,400 4.99
Serum albumin 66,200 4.82
Ovalbumin 45,000 4.65
Carbonic anhydrase 31,000 4.49
Trypsin inhibitor 21,500 4.33
Lysozyme 14,400 4.15
Aprotinin 6,500 3.81
200000.00
116250.00
97400.00
66200.00
45000.00
31000.00
21500.00
14400.00
6500.00

0.5
0.4
0.3
0.2
0.7
0.6
0.9
0.8
0.1
3.70
3.90
4.10
4.30
4.50
4.70
Log Moledular Weight
4.90
5.10
5.30
3-28-01 #1
3-28-01 #2
3-28-01-#3
4-4-01 #1
4-4-01 #2
4-4-01 #3
4-4-01 #4
4-4-01 #5
4-l7-01 #1
4-17-01 #2

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Specific spreadsheet tasks were demonstrated with detailed instructions
Example if desired
Work Files Evolved During First Two
Weeks in Preparation for Projects
Example if desired

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1. During the week of preparation of final presentations, activity level was high.
2. Presentations were excellent.
3. Students were proud of their accomplishment.