Fall, 2015
Houston Community College
Microbiology
(Biol. 2420)
Lecturer: Dr. Ooi
CRN: 70047
M: Rm. 306
W: Rm. 307
8:30-11:30 am
Textbook: Robert W. Bauman. 2014. Microbiology with Diseases by Taxonomy (4th
edition). Benjamin Cummings.
Lab Manual: Wan H. Ooi, 2010. A Laboratory Manual: Microbiology (9th edition).
Royal Publishing.
8-24-2015 (M)
Chapter
1
A Brief History of Microbiology
8-26 (W)
____________
8-31 (M)
3
Cell Structure and Function
4
6
Microscopy, Staining, and Classification
Microbial Nutrition and Growth
9-2(W)
Lab. Exercise 1: Compound Light Microscope
Lab. Exercise 2: Measurement of Microorganisms
____________
9-7 (M)
9-9 (W)
____________
9-14 (M)
9-16 (W)
____________
9-21 (M)
9-23 (W)
____________
9-28 (M)
9-30 (W)
____________
Labor Day
Lab. Exercise 3: A Simple Staining Method: Methylene Blue
Staining
Lab. Exercise 4: Negative Staining Method
First Lecture Exam (Chapters 1, 3, 4, 6)
7
Microbial Genetics
Lab. Exercise 5: Gram's Staining Method
Lab. Exercise 11: Isolation of Pure Cultures
7
8
Microbial Genetics (continued)
Recombinant DNA Technology
9
Controlling Microbial Growth in the Environment
Second Lecture Exam (Chapters 7, 8, 9)
Lab. Exercise 6: Acid-fast Staining
Lab. Exercise 7: Staining of Bacterial Endospores
10-5 (M)
10
10-7 (W)
Lab. Exercise 8: Staining of Bacterial Capsules
Lab. Exercise 9: Staining of Bacterial Flagella
Lab. Exercise 10: Motility Testing
____________
10-12 (M)
10-14(W)
____________
10-19 (M)
11
Controlling Microbial Growth in the Body: Antimicrobial
Drugs
Characterizing and Classifying Prokaryotes
Lab. Exercise 12: Selective and Differential Media
Lab. Exercise 13: Oxygen Requirements of Bacteria
12
Characterizing and Classifying Eukaryotes
10-21 (W)
____________
10-26 (M)
Lab. Exercise 23: Genetic Engineering: Gene Splicing Technique
10-28 (W)
____________
11-2 (M)
First Lab. Exam
11-4 (W)
Lab. Exercise 14: Standard Plate Count (SPC) of Milk
Lab. Exercise 24: Agarose Gel Electrophoresis
____________
11-9 (M)
11-11 (W)
____________
11-16 (M)
11-18 (W)
____________
11-23 (M)
11-25 (W)
____________
Third Lecture Exam (10, 11, 12)
13
14
Characterizing and Classifying Viruses, Viroids, and Prions
Infection, Infectious Diseases and Epidemiology
Lab. Exercise 15: Thermal Death Time (TDT) of Bacteria
Lab. Exercise 16: Determination of Minimal Inhibitory
Concentration (MIC) of a Chemical by Tube
Dilution Method
15
16
Innate Immunity
Adaptive Immunity
Lab. Exercise 17: Fermentation of Carbohydrates
Lab. Exercise 18: Triple Sugar Iron (TSI) Agar Fermentation
Lab. Exercise 26: DNA Fingerprinting
Fourth Lecture Exam (13, 14, 15)
Lab. Exercise 19: The IMViC Tests
Lab. Exercise 20: Quantitation of Bacteriophages
Lab. Exercise 21: Differential Count of White Blood Cells
11-30 (M)
17
18
12-2 (W)
Lab. Exercise 22: Antibiotic Sensitivity Testing: Kirby-Bauer
Method
Lab. Exercise 27: Slide Cultivation of Fungi
____________
12-7 (M)
Immunization and Immune Testing
Immune Disorders
Lecture Final Exam (16, 17, 18)
12-9 (W)
Lab Final Exam
________________________________________________________________________
Lecture (70%)
Lab (30%)
Exam 1----------------------20%
Exam 2----------------------20%
Exam 3----------------------20%
Exam 4 ---------------------20%
Final Exam(90%)---------20%
Dept. Final (10%)
Lab reports and performance---------40%
First Lab Exam-------------------------30%
Lab Final Exam------------------------30%
A: 85-100
D: 50-59
B: 75-84
C: 60-74
F: less than 50