SUPPORTING INFORMATION
Fig S1. The sense and antisense strand sequences of SlMAPK7 RNAi construct. a The sense strand sequence is shadowed for
construction of RNAi vector in gray, and the primer pairs are underlined. b The antisense strand sequence is shadowed, and the
primer pairs are underlined.
Fig S2. The cDNA sequence of SlMAPK7. Underlined oligonucleotides indicate the PCR primers. The initial codon ATG and the
termination codon TGA are boldfaced.
Fig S3. PCR amplification results of expression vector in GV3101. Lane M indicates DNA ladder. a Lane 1, 2, 3: Sense fragment of
p35S::SlMAPK7-RNAi, lane 4, 5, 6: Antisense fragment of p35S::SlMAPK7-RNAi. b Lane 1, 2, 3: Over-expression fragment of
p35S:: SlMAPK7.
Fig S4. Generation of transgenic tomato plants. a Seeding for 6 d. b Pre-culture. c Co-culture. d Explants during differentiation. e
Subculture. f Rooting of shoots. g Regenerated plant before translating. h Regenerated tomato plant.
Fig S5. Detection of transgenic tomato plants by PCR amplification. M indicates DNA maker; lane 1-9 indicate the transgenic
tomato plants with pCAMBIA1301; lane 10 indicates negative control; lane 11 indicates positive control.
Fig S6. Detection of RNAi and overexpression transgenic tomato plants by PCR amplification. M indicates DNA maker. a Lane 1-2
indicate the transgenic tomato plants with p35S::SlMAPK7-RNAi, lane 3 indicates negative control, lane 4 indicates positive control.
b Lane 1-12 indicate the transgenic tomato plants with p35S:: SlMAPK7, lane 13 indicates negative control, lane 14 indicates
positive control.
Fig S7. GUS expression (blue color) in the transgenic tomato by staining method of histochemistry.
a-c The root, leaf and flower
of wild tomato plants, respectively. d-f The root, leaf and flower of transgenic tomato plants, respectively.
Fig S8. Growth of CK-3, 35S-11 and OE -8 transgenic plants. Bars=2cm.
Fig S9. The expression levels of group B MAPK genes in the stamens of RNAi transgenic and control tomato lines using QRT-PCR.
RNAi-11 was selected for further research. Ubi3 was used as an internal control. Standard errors for three independent experiments
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are also shown.
Fig S10. Comparison of the amino acid sequences of SlMAPK7 with AtMAPK4 (a) and AtMAPK11 (b). Sequence alignment shows
that they share high sequence similarity (79% and 78.5%, respectively).
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