Supplemental data_R1
Table S1. Primers used in this study.
Gene Name (Locus)
Sequence(5’ -3’)
AtOCT1_F(At1g73220)
GCGTGATCCGTGCGATATC
AtOCT1_R(At1g73220)
ACCCAACCGCCTCAACTG
AtLAT1_F (At5g05630)
TCTCGGCGTTTTCTCCATCC
AtLAT1_R (At5g05630)
AGCGAAATACCCGTCAGTCC
AtLAT2_F (At3g13620)
TACTCTTGCAGGGGAAGTGG
AtLAT2_R (At3g13620)
AAACCCGTTTTCCCATCTGC
AtLAT3_F (At1g31820)
TTCGGTGCTGAAGGTAGTGT
AtLAT3_R (At1g31820)
ACACCGCAAAGCCATTTCAT
AtLAT4_F (At1g31830)
CTGCTGCATCAAGACCGTAC
AtLAT4_R (At1g31830)
ATTCCCGAGTCTGCTGTTGA
AtLAT5_F (At3g19553)
TGGCTACGTGGTTTGGATCT
AtLAT5_R (At3g19553)
GGAACACGAGCTGCTACATG
AtCBP20_F (At5g44200)
GGCTCATTGGGAGCTTATCCT
AtCBP20_R (At5g44200)
CCACGCCTTCCATTTCCATA
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Fig. S1. Effect of Put pretreatment on the Arabidopsis growth in Cad-containing MS agar medium. (A)
Growth phenotypes of Put-pretreated Arabidopsis WT (Col-0) plants grown on MS agar medium (upper
image) and 1 mM Cad-containing MS agar medium (lower image). Seven-day-old WT (Col-0) plants
grown on a 1/2 MS agar were pretreated with different doses (0.5, 1 and 2 mM) of Put dissolved in 1/2
MS solution for 12 h, then transferred onto 1/2 MS agar medium or 1 mM Cad-containing 1/2 MS agar
medium, and further incubated for 7 days. White bar corresponds to 1 cm. (B) The relative root growth of
Put-pretreated Arabidopsis plants grown on 1/2 MS agar medium (upper figure) and 1 mM
Cad-containing 1/2 MS agar medium (lower figure) for 7 days. The root length of non-pretreated
seedlings was set as 100% and those of Put-treated seedlings were relatively displayed. Experiments were
repeated three times and the mean values + SD were displayed.
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Fig. S2. The relative transcript levels of LAT family members in rmv1 mutant. Sterilized control Ler seeds
and rmv1 seeds were placed on normal 1/2 MS agar medium or 100 μM Cad-containing 1/2 MS agar
medium and incubated for 2 weeks in a growth chamber. The relative LAT2 to LAT5 transcripts were
analyzed by qRT-PCR. The LAT transcript levels of WT (Ler) and rmv1grown in physiological condition
[(-) Cad] were set as 1.0 and the relative transcript levels were displayed. The mean values + SD were
displayed. Asterisk indicates that there is significant difference at *P<0.05, **P<0.01.
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Fig. S3. The relative transcript levels of LAT1 (A) and OCT1 (B) in control transgenic (VC) plants and
LAT1-overexpressed line (OXC1). The plants were grown either on normal 1/2 MS medium or on 50, 100
and 300 μM Cad-containing 1/2 MS agar media. The respective sterilized seeds were placed on the
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described agar media and incubated for 2 weeks. Total RNAs were prepared as described and used for the
qRT-PCR analysis. The inset in (A) has magnified (x 1,250) scale in ordinate. Experiments were repeated
three times and the mean values + SD were displayed. Asterisk indicates that there is significant
difference at *P<0.05, **P<0.01.
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Fig. S4. Growth responses to various PAs of Arabidopsis WT, spms mutant, two lines of SPMS
overexpressing plants, and pao4-1 mutant. (A) Growth response to the higher doses of Cad. (B) Growth
response to 1,6-diaminohexane. (C) Growth response to Put, Spd, Spm and T-Spm. After sowing the
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seeds on the agar plates, the plates were incubated for two weeks at vertical position. White bar
corresponds to 1 cm.
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