1
Figure
2
3
Supplementary Fig. 1 Kinetic bioluminescent intensity of ADPWH_recA induced by artificial Cr-
4
contaminated soils. The soils were all treated with ultrasound for 300 s and exposed to the bioreporter
5
as soil-water slurries. Three replicates were conducted for each soil sample and three independent
6
replicates were carried out for the whole experiment. Error bars were standard deviations.
7
8
9
10
11
12
13
14
15
16
17
18
19
1
20
21
22
Supplementary Fig. 2 Cell viability estimation of ADPWH_recA exposed to different concentrations
23
of Cr(VI). The 100 μM, 10 μM and 1 μM samples were artificial Cr(VI)-contaminated soils and S7
24
was the Cr(VI)-contaminated soils taken from Shandong Province. 200 mg of these soils were mixed
25
with 5 ml Milli-Q water and sonicated for 300 s as water-soil slurries. The cell population estimating
26
was done by colony couting on LB agar plate. Bioreporter cells exposed to different concentrations of
27
Cr(VI) were sampled at 0 h, 3 h, 4 h, 5 h and 6 h, and made a series dilution of 101, 102, 103, 104, 105
28
and 106. The 100 μl of each diluted cells were spread on LB agar plate and incubated at 30 °C for
29
colony counting. Three replicates were carried out for each treatment and three independent replicates
30
were carried out for the whole experiment. Error bars were standard deviations.
31
32
33
34
35
36
37
2
38
39
Supplementary Fig. 3 Sample collection from chromium-contaminated sites in China.
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
3
55
Table
56
Supplementary Table 1. Physical and chemical properties of chromium-contaminated soil samples.
No.
Area
Cr concentration
pH
(ppm)
Organic matter
Cation exchange capacity
(%)
(cmol/100 g)
S2
Shenyang
499
9.35
3.1
21
S4
Henan
112
8.36
2.04
28
S6
Henan
388
7
0.94
28
S7
Shandong
7089
10.72
0.96
22
S8
Shandong
7505
12.06
0.88
31
S13
Hubei
390
9.05
2.73
26
S14
Hubei
499
7.75
2.28
44
S15
Wuxi
123
8.4
0.59
36
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
4
72
Supplementary Table 1. Physical and chemical properties of chromium-contaminated soil samples.
No.
Total
Total
Total
Available
Available
Available
nitrogen
phosphate
potassium
nitrogen
phosphate
potassium
(%)
(%)
(%)
(mg/kg)
(mg/kg)
(mg/kg)
S2
0.071
0.046
1.82
17.3
4.35
223
S4
0.102
0.039
1.75
47.5
0.66
130
S6
0.059
0.03
2.22
37.9
0.62
152
S7
0.004
0.056
0.29
6.4
17.8
57
S8
0.006
0.06
0.12
3.9
23.1
120
S13
0.12
0.044
1.27
36
0.22
93
S14
0.055
0.032
1.65
23.8
1.27
63
S15
0.046
0.065
1.9
37.2
1.57
97
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
5
91
Supplementary Table 1. Physical and chemical properties of chromium-contaminated soil samples.
Particle size distribution (%)
No.
Sand
Silt
Clay
>0.05 mm
0.05–0.02 mm
0.02–0.0063 mm
0.0063–0.002 mm
<0.002 mm
S2
46
16
13.3
7.4
17.7
S4
22
19.60
26.60
11.5
20
S6
21
14.2
18.3
11.2
35.4
S7
77
5.8
9.2
4.4
4.1
S8
64
7.2
13.3
9.1
6.3
S13
37
11.8
26.3
3.6
21.1
S14
35
13.7
24.30
7.8
18.9
S15
19
19.4
29
5
27.2
92
6