Electronic Supplementary Material
Copper nanoclusters as an on-off-on fluorescent probe for ascorbic acid
Hanbing Rao a*, Hongwei Ge a, Zhiwei Lu a,Wei Liu a, Ziqi Chen a, Zhaoyi Zhanga, Xianxiang
Wang a, Ping Zoua, Yanying Wang a*, Hua Heb, Xianying Zengc
a
College of Science, Sichuan Agricultural University, Ya’an 625014, P.R. China
b
Animal Genetics and Breeding Institute of Sichuan Agricultural University, Sichuan Ya’An
625014, P.R. China
c
College of Life Science, Sichuan Agricultural University, Ya’an 625014, P.R. China
The authors wish it to be known that, in their opinions, Hanbing Rao and Hongwei Ge should
be regarded as joint First Authors.
*Corresponding author, Hanbing Rao, E-mail: rhb@sicau.edu.cn or rhbscu@gmail.com,
Yanying Wang, E-mail: 43936939@qq.com
Fig. S1 Fluorescence decays of TA-Cu NCs and TA-CuNCs/Fe(III).
1
Fig. S2 (a) Kinetic of reactions between TA-CuNCs and Fe(III) (black), TA-CuNC/Fe(III)
and AA(red). (b) Dependence of ΔF/F0 of the prepared TA-Cu NCs and TA-Cu NCs/Fe(III)
system on the dosage of TA-CuNCs. (c) Relative fluorescence intensities ΔF/F0 of solutions
of TA-CuNCs in the presence of AA at different concentration of Fe(III) from 20-80μM. (d)
Effects of solution pH on the fluorescence responses of TA-CuNCs in different systems:
TA-CuNCs and Fe(III) (black); TA-CuNC/Fe(III) and AA(red).
2
Fig. S3 Fluorescence response of TA-CuNCs in the presence of variety of ions and molecules.
Table S1. Nanosecond time-resolved luminescence transients of TA-CuNCs in different
systems. The luminescence of TA-CuNCs(maximum wavelength, λmax=430 nm) was detected
with a 355 nm excitation laser.
system
τ1
R2
(ns)
τ
(ns)
TA-CuNCs
1.8369
1.091
1.84
TA-CuNCs + Fe(III)
1.8947
1.008
1.89
3
Table S2 The comparison of the determination of AA
Methods
Fluorescence method with
graphene quantum dots
Fluorescence method with
Au nanoclusters
Fluorescence method with
CdTe quantum dots
Fluorescent carbon dot
nanosensor
HPLC
MnFe2O4@CNT-N
electrochemical
nanosensor
Fluorescence method with
TA-Cu NCs/Fe(III)
Linear detection
range
Detection limit
0.3– 10μM
94
5-100μM
5 μM
[2]
0.3-10μM
74 nM
[3]
Reference
[1]
0.03-0.1mM
[4]
0.2
[5]
2.0 × 10−6 -1.0 × 10−
4
M
1.8 × 10−6 M
[6]
0.5-10μM
0.11 μM
This
work
References
[1] Liu J-J, Chen Z-T, Tang D-S, Wang Y-B, Kang L-T, Yao J-N (2015) Graphene quantum dots-based
fluorescent probe for turn-on sensing of ascorbic acid. Sens. Actuators, B 212:214-219
[2] Hu L, Deng L, Alsaiari S, Zhang D, Khashab N M (2014) “Light-on” Sensing of Antioxidants
Using Gold Nanoclusters. Anal. Chem. 86(10):4989-4994
[3] Chen Y J, Yan X P (2009) Chemical redox modulation of the surface chemistry of CdTe quantum
dots for probing ascorbic acid in biological fluids. Small 5(17):2012-2018
[4] Zheng M, Xie Z, Qu D, Li D, Du P, Jing X, Sun Z (2013) On-off-on fluorescent carbon dot
nanosensor for recognition of chromium(VI) and ascorbic acid based on the inner filter effect. ACS
Appl Mater Interfaces 5(24):13242-13247
[5] Stan M, Soran M L, Marutoiu C (2014) Extraction and HPLC determination of the ascorbic acid
content of three indigenous spice plants. J. Anal. Chem. 69(10):998-1002
[6] Fernandes D M, Silva N, Pereira C, Moura C, Magalhães J M C S, Bachiller-Baeza B,
Rodríguez-Ramos I, Guerrero-Ruiz A, Delerue-Matos C, Freire C (2015) MnFe2O4@CNT-N as novel
electrochemical nanosensor for determination of caffeine, acetaminophen and ascorbic acid. Sens.
Actuators, B 218:128-136
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