Table S1. Experimental methods and statistical methods
Index
DNA Extraction
PCR
Amplification
Typing
Method
Chelex-100 method
AGCU Expressmarker 20 fluorescence amplification
reagents (AGCU ScienTech Inc., Wuxi, Jiangsu, China) with
the following conditions: initial denaturation at 95°C for 2
min, followed by 10 cycles of 94°C for 30s, 60°C for 1min,
72°C for 1min and 20 cycles of 90°C for 30s, 58°C for 1min,
72°C for 1min, and additional 10 min at 72°C.
ABI PRISM 3130 Genetic Analyzer (Applied Biosystems,
Foster City, CA, USA) and GeneMapper ID 3.2 software
(Applied Biosystems, Foster City, CA, USA) was used.
9947A cell line (Promega, Madison, WI, USA) DNA was
also genotyped as control.
Quality Control
According to the kit control and the ISO 17025 standard
Analysis of Data
Allelic frequencies, forensic statistical parameters and the
exact tests of HWE, the modified powerstate (version1.2)
spreadsheet.
Exact tests of LD between all pairs of STR loci, Genepop
version 4.0.10.
AMOVA, ARLEQUIN software version 3.1.
PCA, MATLAB 2007a (MathWorks).
Reference
Walsh et al (1991)
* HWE, Hardy-Weinberg equilibrium; LD, linkage disequilibrium; AMOVA, analysis of
molecular variance; PCA, principal component analysis
Reference of Table S1

Walsh PS, D.A.Metzger, R.Higuchi (1991) Chelex 100 as a medium for simple extraction of
DNA for PCR-based typing from forensic material. Biotechniques 10: 506-513.