Supplemental Figure 1. Gene levels of LDP miR-103b and its target, SFRP4, in an individual patient
(A and B) qRT-PCR analysis showed that platelet-derived miR-103b negatively regulated SFRP4
expression in pre-diabetes patients. Pre-diabetic patients (n=19) vs. healthy control (n=19). qRT-PCR
data were analyzed using the 2−ΔΔCt method. Validation of sequence-specific miR-103b and SFRP4
expression was carried out as described previously. Values are presented as the mean ± SEM. *
p<0.05 vs. control.
Supplemental Table S1 Baseline characteristics of the study population
Data are shown as the mean ± standard deviation. Healthy controls, diabetes-free subjects; pre-DM,
patients with pre-diabetic mellitus; NCDM, patients with non-complicated diabetic; DM-CHD,
patients with diabetes mellitus type 2-coronary heart disease; BMI, body mass index; TC, total
cholesterol; TG, triglyceride; HDL-C, high-density lipoprotein cholesterol; LDL-C, low-density
lipoprotein cholesterol; HbA1c, hemoglobin A1c; GLU, Fasting blood glucose; BP, blood pressure;
WC, waist circumference.
Supplemental Table S2 Sequences of primers used in the study