ST 524 Multiple Comparisons Procedures NCSU - Fall 2008 Cherry Spraying Example Nine sprays were tested for their ability to hold fruit on the tree, the fruit crop being cherries. The experimental design was a randomized complete block design with 9 blocks. Plots were single trees. The number of trees in dour one-pound random samples of the crop from each of the 81 trees was obtained. An average count was obtained for the four samples from each tree. The data are reported below. TRT T1 T2 T3 T4 T5 T6 T7 T8 T9 REP1 REP2 REP3 REP4 REP5 REP6 REP7 REP8 REP9 126.50 117.75 145.00 109.50 124.25 128.50 117.00 113.75 123.50 111.00 116.00 179.50 119.50 120.75 121.00 128.75 112.75 126.75 113.00 104.25 159.50 121.25 118.50 131.50 123.75 111.25 126.50 113.25 110.75 125.75 109.25 125.00 134.75 119.00 114.25 117.25 117.00 114.75 149.00 104.25 123.25 129.00 115.50 109.00 117.50 106.75 107.00 139.75 114.25 132.75 124.00 110.50 119.75 107.50 115.00 117.00 145.75 120.50 127.25 106.75 117.50 117.00 115.50 98.75 126.50 142.75 103.50 114.25 113.00 118.75 104.50 121.50 113.75 113.50 179.50 107.25 128.75 127.75 101.50 106.25 121.00 Mean and variance of Number of fruit (count/lb) per tree Cherry Spraying var_ range_ Obs trt mn_count count count 1 2 3 4 5 6 7 8 9 10 T1 T2 T3 T4 T5 T6 T7 T8 T9 120.827 112.778 114.167 151.833 112.139 123.861 124.028 116.917 112.056 119.667 212.037 56.069 42.313 323.781 48.455 30.877 82.335 59.453 24.075 36.578 80.75 27.75 22.25 53.75 17.75 18.50 28.00 27.25 15.25 19.25 Decision was taken to drop treatment 3 from analysis. This spray was found to destroy the flesh of the cherry, leaving only the stone. Analysis of variance and Multiple Comparison Procedures to analyze treatment effects. ThursdayOctober 16, 2008 Multiple Comparisons Procedures 1 ST 524 Multiple Comparisons Procedures NCSU - Fall 2008 Number of fruit (count/lb) cherries per tree Cherry Spraying - Multiple Comparison Procedures 27 The GLM Procedure Class Level Information Class Levels rep trt Values 9 8 1 2 3 4 5 6 7 8 9 T1 T2 T4 T5 T6 T7 T8 T9 Number of Observations Read Number of Observations Used 72 72 Dependent Variable: count Source DF Sum of Squares Mean Square F Value Pr > F Model 15 1973.204861 131.546991 2.76 0.0030 Error 56 2665.500000 47.598214 Corrected Total 71 4638.704861 Ho: All spray treatments have same effect R-Square Coeff Var Root MSE count Mean 0.425378 5.899157 6.899146 116.9514 Source rep trt Source rep trt Ho: i 0 DF Type I SS Mean Square F Value Pr > F 8 7 375.736111 1597.468750 46.967014 228.209821 0.99 4.79 0.4562 0.0003 DF Type III SS Mean Square F Value Pr > F 8 7 375.736111 1597.468750 46.967014 228.209821 0.99 4.79 0.4562 0.0003 , i 1, t Tests (LSD) for count NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 56 Error Mean Square 47.59821 Critical Value of t 2.00324 Least Significant Difference 6.5151 t(0.975, 56) lsd0.05,56df 2.00324 47.59821 2 9 Means with the same letter are not significantly different. t Grouping Mean N trt A A A A A 124.028 9 T6 123.861 9 T5 119.667 9 T9 C C C C C C C C C 116.917 9 T7 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 B B B B B ThursdayOctober 16, 2008 Multiple Comparisons Procedures 2 9 ST 524 Multiple Comparisons Procedures NCSU - Fall 2008 Waller-Duncan test The GLM Procedure Waller-Duncan K-ratio t Test for count Note: This test minimizes the Bayes risk under additive loss and certain other assumptions. Kratio 100 Error Degrees of Freedom 56 Error Mean Square 47.59821 F Value 4.79 Critical Value of t 2.01945 Minimum Significant Difference 6.5678 Means with the same letter are not significantly different. Waller Grouping Mean N trt 124.028 9 T6 123.861 9 T5 A 119.667 9 T9 B C 116.917 9 T7 B C B C 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 A A A A B B C C C C C C ThursdayOctober 16, 2008 Multiple Comparisons Procedures 3 ST 524 Multiple Comparisons Procedures NCSU - Fall 2008 Duncan's Multiple Range Test for count NOTE: This test controls the Type I comparisonwise error rate, not the experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 56 Error Mean Square 47.59821 Number of Means Critical Range 2 6.515 3 6.853 4 7.076 5 7.238 6 7.363 7 7.462 8 7.544 Means with the same letter are not significantly different. Duncan Grouping Mean N trt A A A A A 124.028 9 T6 123.861 9 T5 119.667 9 T9 C C C C C C C C C 116.917 9 T7 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 B B B B B B B > 124.028 - 123.861 0.167 compared to > 124.028 - 116.917 7.111 compared to > 119.667 - 112.139 7.528 compared to = 0.167 6.515 = 7.111 7.076 = 7.528 7.238 Tukey's Studentized Range (HSD) Test for count NOTE: This test controls the Type I experimentwise error rate, but it generally has a higher Type II error rate than REGWQ. Alpha 0.05 Error Degrees of Freedom 56 Error Mean Square 47.59821 Critical Value of Studentized Range 4.45236 Minimum Significant Difference 10.239 > qtukey, 0.05, 8 trt, w q p, f e s y 56 df Means with the same letter are not significantly different. Tukey Grouping Mean N trt A A A A A A A A A 124.028 9 T6 123.861 9 T5 119.667 9 T9 116.917 9 T7 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 B B B B B B B B B B B ThursdayOctober 16, 2008 Multiple Comparisons Procedures 4 ST 524 Multiple Comparisons Procedures NCSU - Fall 2008 Student-Newman-Keuls Test for count NOTE: This test controls the Type I experimentwise error rate under the complete null hypothesis but not under partial null hypotheses. Alpha 0.05 Error Degrees of Freedom 56 Error Mean Square 47.59821 Number of Means Critical Range 2 6.5152872 3 7.8301065 4 8.6118701 5 9.1670319 6 9.5964097 7 9.9455437 8 10.239151 Means with the same letter are not significantly different. SNK Grouping Mean N trt A A A A A A A 124.028 9 T6 123.861 9 T5 119.667 9 T9 116.917 9 T7 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 B B B B B B B B B B B Number of fruit (count/lb) cherries per tree Cherry Spraying - Multiple Comparison Procedures 34 Bonferroni (Dunn) t Tests for count NOTE: This test controls the Type I experimentwise error rate, but it generally has a higher Type II error rate than REGWQ. Alpha 0.05 Error Degrees of Freedom 56 Error Mean Square 47.59821 Critical Value of t 3.28057 Minimum Significant Difference 10.669 2 k k 1 0.001785 t10.001785/ 2,56 df 3.28057 Means with the same letter are not significantly different. Bon Grouping Mean N trt A A A A A A A A A 124.028 9 T6 123.861 9 T5 119.667 9 T9 116.917 9 T7 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 Sidak: 1 1 2 k k 1 0.001830 t10.001830/ 2,56 df 3.27227 B B B B B B B B B B B ThursdayOctober 16, 2008 Multiple Comparisons Procedures 5 ST 524 Multiple Comparisons Procedures NCSU - Fall 2008 Scheffe's Test for count NOTE: This test controls the Type I experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 56 Error Mean Square 47.59821 Critical Value of F 2.17816 Minimum Significant Difference 12.699 Means with the same letter are not significantly different. Scheffe Grouping Mean N trt A A A A A A A A A A A A A A A 124.028 9 T6 123.861 9 T5 119.667 9 T9 116.917 9 T7 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 Ryan-Einot-Gabriel-Welsch Multiple Range Test for count NOTE: This test controls the Type I experimentwise error rate. Alpha 0.05 Error Degrees of Freedom 56 Error Mean Square 47.59821 Number of Means Critical Range 2 8.3699169 3 9.0960312 4 9.4906375 5 9.7573197 6 9.9553297 7 9.9553297 8 10.239151 Means with the same letter are not significantly different. REGWQ Grouping Mean N trt A A A A A A A 124.028 9 T6 123.861 9 T5 119.667 9 T9 116.917 9 T7 114.167 9 T2 112.778 9 T1 112.139 9 T4 112.056 9 T8 B B B B B B B B B B B ThursdayOctober 16, 2008 Multiple Comparisons Procedures 6 ST 524 Multiple Comparisons Procedures ThursdayOctober 16, 2008 Multiple Comparisons Procedures NCSU - Fall 2008 7