Academic Scientists at Work
Peptide Database System
Appendix 4-4
The Peptide File system is designed to help you organize and find your reagents over the course
of your lifetime. Both Macintosh and PC versions written with FileMaker Pro version 7.0 are
included. The file was designed in Dr. Boss’ laboratory and is in use. Modifications have been
made to make it more general. The program can be modified and expanded or used as is.
Below is a discussion of different views and the fields:
Peptide File View - This is the data entry and general search view that you should use. The fields
are discussed.
To enter a new record, click on “New Peptide.”
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Peptide Name: Give it a meaningful name. If you are making a series of peptides from
the same protein, names like CYC27-48, meaning cytochrome c amino acids 27 through
48, is very clear. This is better than Joey23 for Joey’s 23 rd peptide.
Creator: This is a Pop-up menu that allows you to make up a list for easy entry and ID.
An edit function allows you to make changes.
Sequence: Enter here.
Gene: Enter gene abbreviation.
N Term and C Term: Enter the positions of the ends of the peptide. This may come in
real handy when you review this material years later.
Region of Protein: Indicate if the peptide is in the N-terminal region, in a DNA-binding
domain, in an SH2 domain, etc…
Gene Reference: Place reference information here that may be useful later.
Molecular Weight: Useful for all calculations later. Often this is supplied with the
peptide and is easily entered when the peptide arrives (before the label wears off or the
sample is transferred).
Purification: Pop-up menu to indicate if the peptide was HPLC purified or purified by
some other method.
Concentration: Enter the concentration that the peptide stock is now in.
Moles/mg: Will be helpful later, but not necessary if you put in the molecular weight
Location: Pop-up menu
List View – Allows you to print all the peptides out for a complete inventory. You can also sort
and find in this category as well.
Print out this sheet from the CD to keep with your hard copies of your peptide
inventory